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Embryogenic callus, cell suspension and protoplast cultures of cereals

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Plant Tissue Culture Manual

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Abstract

Cereals constitute the most important source of calories and protein for man since more than 52% of our food is derived from grains such as wheat, rice, maize, barley, millets, etc. Cereals, therefore, are an obvious and important target for genetic manipulation by modern biotechnological methods which require efficient regeneration of plants from cultured tissues and cells, and genetic transformation, two important and interacting components of plant biotechnology. In addition, owing to the serious problems still being faced in Agrobacterium-mediated transformation of cereals, the development of a reliable protoplast regeneration system for direct DNA delivery is also a necessity.

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Author information

Authors and Affiliations

  1. Laboratory of Plant Cell and Molecular Biology, Department of Vegetable Crops, University of Florida, Gainesville, FL, 32611-0514, USA

    Indra K. Vasil & Vimla Vasil

Authors
  1. Indra K. Vasil
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  2. Vimla Vasil
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Editor information

Editors and Affiliations

  1. Department of Biological Sciences, University of Durham, Leicester, UK

    K. Lindsey

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© 1991 Springer Science+Business Media New York

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Vasil, I.K., Vasil, V. (1991). Embryogenic callus, cell suspension and protoplast cultures of cereals. In: Lindsey, K. (eds) Plant Tissue Culture Manual. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0103-2_12

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  • DOI: https://doi.org/10.1007/978-94-009-0103-2_12

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-011-7658-3

  • Online ISBN: 978-94-009-0103-2

  • eBook Packages: Springer Book Archive

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