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Characterization and regeneration of wheat (Triticum aestivum L.) embryogenic cell suspension cultures

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Summary

Stable cell suspension cultures were established from two types of calli (one compact, nodular and embryogenic, the other friable and embryogenic) derived from cultured immature embryos of wheat (cv FLA302). Only aged calli, which had been subcultured for at least 5–8 months, formed suspensions comprised mainly of groups of small, round, densely cytoplasmic, starch-containing cells. Only the embryogenic suspension derived from the aged, compact and nodular callus formed distinct somatic embryos when plated on regeneration media containing IAA and zeatin. Upon subsequent transfer to fresh regeneration medium more than 200 green rooted plants were obtained.

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Abbreviations

6-BA:

6-benzylaminopurine

CH:

casein hydrolysate

2,4-D:

2,4-dichlorophenoxyacetic acid

GA3 :

gibberellic acid

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

MS:

Murashige and Skoog (1962) basal medium

NAA:

naphthaleneacetic acid

PCV:

packed cell volume

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Authors and Affiliations

  1. Laboratory of Plant Cell and Molecular Biology, Department of Vegetable Crops, University of Florida, 32611-0514, Gainesville, FL, USA

    Flona A. Redway, Vimla Vasil & Indra K. Vasil

Authors
  1. Flona A. Redway
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  2. Vimla Vasil
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  3. Indra K. Vasil
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Communicated by E. D. Earle

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Redway, F.A., Vasil, V. & Vasil, I.K. Characterization and regeneration of wheat (Triticum aestivum L.) embryogenic cell suspension cultures. Plant Cell Reports 8, 714–717 (1990). https://doi.org/10.1007/BF00272101

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  • DOI: https://doi.org/10.1007/BF00272101

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