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Raman Micro-spectral Imaging of Cells and Intracellular Drug Delivery Using Nanocarrier Systems

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Confocal Raman Microscopy

Part of the book series: Springer Series in Surface Sciences ((SSSUR,volume 66))

Abstract

Raman microscopic imaging of eukaryotic cells has become a well-established technique to address various biochemical questions related to cell biology. The combination of Raman spectroscopy with optical microscopy offers the possibility to study the composition of cells and alterations associated with metabolistic activity induced by internal or external factors. Because of the associated spectral information obtained, Raman microscopy can be utilized without the need for fluorescent dyes or laborious sample preparation. In order to contrast the cellular compartments within the cytosol, imaging algorithms based on multivariate data analysis are commonly employed. Apart from imaging the intracellular organelles and structures of eukaryotes based on their intrinsic biochemical composition, it is possible to follow the uptake of various substances. Externally administered molecules can be labeled with stable isotopes, such as deuterium. The unique spectroscopic features of isotopically labeled compounds provide high specificity and sensitivity without changing the chemical properties of the molecules of interest. The concept of isotopic labeling is illustrated for the uptake of lipids into macrophages , as well as for liposomal drug delivery systems.

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References

  1. A. García-Sáez, P. Schwille, Appl. Microbiol. Biotechnol. 76(2), 257 (2007)

    Article  Google Scholar 

  2. C. Lim, B. Cho, BMB. Rep. 46(4), 188 (2013)

    Article  Google Scholar 

  3. C. Grienberger, A. Konnerth, Neuron 73(5), 862 (2012)

    Article  Google Scholar 

  4. C. Hsieh, S. Chen, Y. Lee, Y. Yang, C. Sun, Opt. Express 16(15), 11574 (2008)

    Google Scholar 

  5. F. Festy, S. Ameer-Beg, T. Ng, K. Suhling, Mol. Biosyst. 3(6), 381 (2007)

    Article  Google Scholar 

  6. N. Sijtsema, A. Tibbe, I. Segers-Nolten, A. Verhoeven, R. Weening, J. Greve, C. Otto, Biophys. J. 78(5), 2606 (2000). http://www.biophysj.org/cgi/content/abstract/78/5/2606

  7. C. Otto, N. Sijtsema, J. Greve, Eur. Biophys. J. 27(6), 582 (1998)

    Article  Google Scholar 

  8. H.J. van Manen, Y. Kraan, D. Roos, C. Otto, Proc. Natl. Acad. Sci. USA 102(29), 10159 (2005). https://doi.org/10.1073/pnas.0502746102. http://www.pnas.org/cgi/content/abstract/102/29/10159

  9. B. Wood, L. Hammer, L. Davis, D. McNaughton, J. Biomed. Opt. 10(1), 014005 (2005). http://link.aip.org/link/?JBO/10/014005/1

  10. S. Verrier, I. Notingher, J. Polak, H.L.L., Biopolymers 74(1–2), 157 (2004). https://doi.org/10.1002/bip.20063

  11. K. Nithipatikom, M.J. McCoy, S. Hawi, K. Nakamoto, F. Adar, W. Campbell, Anal. Biochem. 322, 198 (2003). http://www.ingentaconnect.com/content/els/00032697/2003/00000322/00000002/art00521. https://doi.org/10.1016/.ab.2003.07.020

  12. N. Uzunbajakava, A. Lenferink, Y. Kraan, E. Volokhina, G. Vrensen, J. Greve, C. Otto, Biophys. J. 84(6), 3968 (2003). http://www.biophysj.org/cgi/content/abstract/84/6/3968

  13. N. Uzunbajakava, C. Otto, Opt. Lett. 28(21), 2073 (2003)

    Article  ADS  Google Scholar 

  14. C. Matthäus, S. Boydston-White, M. Miljkovićć, M. Romeo, M. Diem, Appl. Spectrosc. 60(1), 1 (2006)

    Article  ADS  Google Scholar 

  15. N.M. Sijtsema, C. Otto, G. Segers-Nolten, A. Verhoeven, G.J., Biophys. J. 74(6), 3250 (1998). http://www.biophysj.org/cgi/content/abstract/74/6/3250

  16. B. Wood, D. McNaughton, Expert. Rev. Proteomics. 3, 525 (2006). http://www.ingentaconnect.com/content/ftd/epr/2006/00000003/00000005/art00008. https://doi.org/10.1586/14789450.3.5.525

  17. A. Feofanov, A. Grinchine, L. Shitova, T. Karmakova, R. Yakubovskaya, M. Egret-Charlier, P. Vigny, Biophys. J. 78(1), 499 (2000)

    Article  Google Scholar 

  18. S. Arzhantsev, A. Chikishev, N. Koroteev, J. Greeve, C. Otto, N. Sijtsema, J. Raman Spectrosc. 30, 205 (1999)

    Article  ADS  Google Scholar 

  19. C. Krafft, T. Knetschke, R. Funk, R. Salzer, Anal. Chem. 78(13), 4424 (2006). http://pubs3.acs.org/acs/journals/doilookup?in_doi=10.1021/ac060205b

  20. C. Matthäus, T. Chernenko, J. Newmark, C. Warner, M. Diem, Biophys. J. 93(2), 668 (2007). https://doi.org/10.1529/biophysj.106.102061. http://www.biophysj.org/cgi/content/abstract/biophysj.106.102061v1

  21. N. Keshava, Lincoln. Lab. J. 14(1), 55 (2003)

    Google Scholar 

  22. J. Nascimento, J. Bioucas Dias, IEEE Trans. Geosci. Remote. Sens. 43(4), 898 (2005). 0196–2892

    Google Scholar 

  23. M. Miljković, T. Chernenko, M. Romeo, B. Bird, C. Matthäus, M. Diem, Analyst 135(8), 2002 (2010)

    Google Scholar 

  24. M. Hedegaard, C. Matthäus, S. Hassing, C. Krafft, M. Diem, J. Popp, Theor. Chem. Acc. 130, 1249 (2011)

    Article  Google Scholar 

  25. B. Kann, H. Offerhaus, M. Windbergs, C. Otto, Adv. Drug. Deliv. Rev. 15(89), 71 (2015)

    Article  Google Scholar 

  26. V. Torchilin, Annu. Rev. Biomed. Eng. 8, 343 (2006)

    Article  Google Scholar 

  27. C. Krafft, M. Kirsch, C. Beleites, G. Schackert, R. Salzer, Anal. Bioanal. Chem. 389(4), 1133 (2007)

    Article  Google Scholar 

  28. B. de Jong, T. Schut, K. Maquelin, T. van der Kwast, C. Bangma, D. Kok, G. Puppels, Anal. Chem. 78(22), 7761 (2006)

    Article  Google Scholar 

  29. C. Krishna, G. Sockalingum, R. Bhat, L. Venteo, P. Kushtagi, M. Pluot, M. Manfait, Anal. Bioanal. Chem. 387(5), 1649 (2007)

    Article  Google Scholar 

  30. C. Chang, IEEE Trans. Geosci. Remote Sens. 43(3), 502 (2005)

    Article  ADS  Google Scholar 

  31. M. Winter, Proc. SPIE 3753, 266 (1999)

    Article  ADS  Google Scholar 

  32. M. Schnoor, P. Cullen, J. Lorkowski, K. Stolle, H. Robenek, D. Troyer, J. Rauterberg, S. Lorkowski, J. Immunol. 180, 5707 (2008)

    Article  Google Scholar 

  33. M. Schnoor, I. Buers, A. Sietmann, M. Brodde, O. Hofnagel, H. Robenek, S. Lorkowski, J. Immunol. Methods 344, 190 (2009)

    Article  Google Scholar 

  34. S. Dipali, S. Kulkarni, G. Betageri, J. Pharm. Pharmacol. 48(11), 1112 (1996)

    Article  Google Scholar 

  35. W. Liang, T. Levchenko, V. Torchilin, J. Microencapsul. 21, 151 (2004)

    Article  Google Scholar 

  36. V. Torchilin, T. Levchenko, A. Lukyanov, B. Kwah, A. Klibanov, R. Rammohan, G. Samokhin, K. Whiteman, Biochim. Biophys. Acta. 1511, 397 (2001)

    Article  Google Scholar 

  37. D. Shenoy, M. Amiji, Int. J. Pharm. 293(1–2), 261 (2005)

    Article  Google Scholar 

  38. D. Naumann, Appl. Spectrosc. Rev. 36(2–3), 239 (2001)

    Article  ADS  Google Scholar 

  39. T.G. Spiro, Biological Applications of Raman Spectroscopy, vol. 1 (Wiley, 1987)

    Google Scholar 

  40. C. Yu, E. Gestl, K. Eckert, D. Allara, J. Irudayaraj, Cancer. Detect. Prev. 30(6), 515 (2006). http://www.sciencedirect.com/science/article/B6X28-4MCW9N6-4/1/f1be1eb8a2bd6bcbd84c3bc8a20ed470

  41. K. Short, S. Carpenter, J. Freyer, J. Mourant, Biophys. J. 88(6), 4274 (2005). https://doi.org/10.1529/biophysj.103.038604. http://www.biophysj.org/cgi/content/abstract/88/6/4274

  42. T. Collins, M. Bootman, J. Exp. Biol. 206(12), 1993 (2003). https://doi.org/10.1242/jeb.00244. http://jeb.biologists.org/cgi/content/abstract/206/12/1993

  43. C. Matthäus, T. Chernenko, L. Quintero, L. Milan, A. Kale, M. Amiji, V. Torchilin, M. Diem, in Biophotonics: Photonic Solutions for Better Health Care, vol. 6991 (SPIE, 2008), vol. 6991, pp. 699,106–108. http://link.aip.org/link/?PSI/6991/699106/1

  44. J. van Blerkom, Reproduction 128, 269 (2004)

    Article  Google Scholar 

  45. C. Warner, J. Newmark, M. Comiskey, S. De Fazio, D. O’Malley, M. Rajadhyaksha, D. Townsend, S. McKnight, B. Roysam, P. Dwyer, C. DiMarzio, Reprod. Fertil. Dev. 16, 729 (2004)

    Article  Google Scholar 

  46. C. Stiebing, C. Matthäus, C. Krafft, A. Keller, K. Weber, S. Lorkowski, J. Popp, Anal. Bioanal. Chem. 406(27), 7037 (2014)

    Article  Google Scholar 

  47. C. Matthäus, C. Krafft, B. Dietzek, B. Brehm, S. Lorkowski, J. Popp, Anal. Chem. 84(20), 8549 (2012)

    Article  Google Scholar 

  48. V. Torchilin, Eur. J. Pharm. Sci. 11(Supplement 2), S81 (2000). http://www.sciencedirect.com/science/article/B6T25-41KPF70-9/1/5e8f6c99b36e1d549dcab7bb408d2213

  49. V. Torchilin, Adv. Drug. Deliv. Rev. 57(1), 95 (2005)

    Article  Google Scholar 

  50. C. Plank, W. Zauner, E. Wagner, Adv. Drug. Deliv. Rev. 34(1), 21 (1998)

    Article  Google Scholar 

  51. G. Kaul, M. Amiji, Pharm. Res. 22(6), 951 (2005)

    Article  Google Scholar 

  52. J. Panyam, V. Labhasetwar, Adv. Drug. Deliv. Rev. 55(3), 329 (2003)

    Article  Google Scholar 

  53. J. Chawla, M. Amiji, Int. J. Pharm. 249(1–2), 127 (2002)

    Article  Google Scholar 

  54. V. Torchilin, Nat. Rev. Drug. Discov. 4, 145 (2005)

    Article  Google Scholar 

  55. L. Serpe, M. Guido, R. Canaparo, E. Muntoni, R. Cavalli, P. Panzanelli, C. Della Pepal, A. Bargoni, A. Mauro, M. Gasco, M. Eandi, G. Zara, J. Nanosci. Nanotechnol. 6(9–10), 3062 (2006)

    Article  Google Scholar 

  56. N. Rao, V. Gopal, Biosci. Rep. 26(4), 301 (2006)

    Article  Google Scholar 

  57. U. Huth, R. Schubert, R. Peschka-Süss, J. Control Release 110(3), 490 (2006)

    Article  Google Scholar 

  58. J. Wadia, R. Stan, S. Dowdy, Nat. Med. 10(3), 310 (2004)

    Article  Google Scholar 

  59. C. Matthäus, A. Kale, T. Chernenko, V. Torchilin, M. Diem, Mol. Pharm. 5(2), 287 (2008)

    Article  Google Scholar 

  60. J. Kristl, B. Volk, P. Ahlin, K. Gombac, M. Sentjurc, Int. J. Pharm. 256, 133 (2003)

    Article  Google Scholar 

  61. J. Wojewodzka, G. Pazdzior, M. Langner, Chem. Phys. Lipids 135(2), 181 (2005)

    Article  Google Scholar 

  62. C. Palmerini, C. Cametti, S. Sennato, D. Gaudino, E. Carlini, F. Bordi, G. Arienti, J. Membr. Biol. 211, 185 (2006)

    Article  Google Scholar 

  63. E. Kang, J. Robinson, K. Park, J. Cheng, J. Control Release 122(3), 261 (2007)

    Article  Google Scholar 

  64. A. Belu, C. Mahoney, K. Wormuth, J. Control Release 126(2), 111 (2008)

    Article  Google Scholar 

  65. D. Edwards, J. Hanes, G. Caponetti, J. Hrkach, A. Ben-Jebria, M. Eskew, J. Mintzes, D. Deaver, N. Lotan, R. Langer, Science 276(5320), 1868 (1997)

    Article  Google Scholar 

  66. L. Siskind, J. Bioenerg. Biomembr. 37(3), 143 (2005)

    Article  Google Scholar 

  67. A. Struckhoff, R. Bittman, M. Burow, S. Clejan, S. Elliott, T. Hammond, Y. Tang, B. Beckman, J. Pharmacol. Exp. Ther. 309(2), 523 (2004)

    Article  Google Scholar 

  68. E. Gulbins, H. Grassmé, Biochim. Biophys. Acta. 1585(2–3), 139 (2002)

    Article  Google Scholar 

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Acknowledgements

We would like to thank Judy Newmark and Carol Warner for their inspirations and help with the oocyte images. Partial support for this research was provided by the NIH (Grant No. CA 090346 to M.D.). Funding from the IGERT nanomedicine education program (to T.C. and L.M.) is gratefully acknowledged. We gratefully acknowledge the financial support by the Carl Zeiss Stiftung and the “Jenaer Biochip Initative 2.0” (JBCI 2.0). The project “JBCI 2.0” (03IPT513Y) within the framework “InnoProfile-Transfer -Unternehmen Region” is supported by the Federal Ministry of Education and Research (BMBF), Germany.

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Authors and Affiliations

  1. Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA, 02115, USA

    Christian Matthäus, Tatyana Chernenko, Miloš Miljković & Max Diem

  2. College of Engineering, University of Puerto Rico, Mayagües, PR, 00680, USA

    Luis Quintero

  3. Department of Pharmaceutical Sciences and Center for Pharmaceutical Biotechnology and Nanomedicine, Northeastern University, Boston, MA, 02115, USA

    Lara Milane, Amit Kale, Mansoor Amiji & Vladimir Torchilin

  4. Leibniz Institute of Photonic Technology (Leibniz-IPHT), Albert -Einstein -Straße 9, 07745, Jena, Germany

    Christian Matthäus, Clara Stiebing & Jürgen Popp

  5. Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany

    Christian Matthäus, Clara Stiebing & Jürgen Popp

  6. Institute of Nutrition and Abbe Center of Photonics, Friedrich Schiller University Jena, Dornburger Straße 25, 07743, Jena, Germany

    Stefan Lorkowski

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  1. Christian Matthäus
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  2. Tatyana Chernenko
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Correspondence to Christian Matthäus .

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Editors and Affiliations

  1. Wissenschaftliche Instrumente und Technologie GmbH, Ulm, Germany

    Jan Toporski

  2. Ulm, Germany

    Thomas Dieing

  3. Wissenschaftliche Instrumente und Technologie GmbH, Ulm, Germany

    Olaf Hollricher

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Matthäus, C. et al. (2018). Raman Micro-spectral Imaging of Cells and Intracellular Drug Delivery Using Nanocarrier Systems. In: Toporski, J., Dieing, T., Hollricher, O. (eds) Confocal Raman Microscopy. Springer Series in Surface Sciences, vol 66. Springer, Cham. https://doi.org/10.1007/978-3-319-75380-5_13

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