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Strain Development by Whole-Cell Directed Evolution

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Directed Enzyme Evolution: Advances and Applications

Abstract

Due to limited knowledge of complicated cellular networks, directed evolution has played critical roles in strain improvement, especially for complex traits with hundreds of genetic determinants and for organisms with few genetic tools. Directed evolution mimics natural evolution in the laboratory via iterative cycles of diversity generation and functional selection or screening to isolate evolved mutants with desirable phenotypes. In this chapter, we summarize recent technological advances and applications of directed evolution in strain development, focusing on the efforts for accelerating evolution workflows, expanding the range of target phenotypes, and facilitating mechanistic understanding of evolved mutations.

$Tong Si and Jiazhang Lian contributed equally to this work

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    Authors and Affiliations

    1. Carl R Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 1206 W Gregory Dr, Urbana, IL, 61801, USA

      Tong Si, Jiazhang Lian & Huimin Zhao

    2. Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 600 South Mathews Ave, Urbana, IL, 61801, USA

      Jiazhang Lian & Huimin Zhao

    3. Departments of Chemistry, Biochemistry, and Bioengineering, University of Illinois at Urbana-Champaign, 600 South Mathews Ave, Urbana, IL, 61801, USA

      Huimin Zhao

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    1. Department of Biocatalysis, Institute of Catalysis and Petrochemistry, CSIC, Madrid, Spain

      Miguel Alcalde

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    Si, T., Lian, J., Zhao, H. (2017). Strain Development by Whole-Cell Directed Evolution. In: Alcalde, M. (eds) Directed Enzyme Evolution: Advances and Applications. Springer, Cham. https://doi.org/10.1007/978-3-319-50413-1_7

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