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Directed Evolution of Unspecific Peroxygenase

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Directed Enzyme Evolution: Advances and Applications

Abstract

Unspecific peroxygenase (UPO) is a heme-thiolate peroxidase with mono(per)oxygenase activity for the selective oxyfunctionalization of C-H bonds. Fueled by catalytic concentrations of H2O2, which acts as both oxygen donor and as final electron acceptor, this stable, soluble, and extracellular enzyme is a potential biocatalyst for dozens of transformations that are of considerable interest in organic synthesis. In this chapter we describe the main attributes of this versatile enzyme while reflecting on the directed evolution campaigns recently followed in our laboratory that set out to enhance the functional expression of UPO in yeast and improve the activity, as well as approximating its properties to the required industrial standards.

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Acknowledgments

We acknowledge the funding and financial support obtained from the European Commission projects FP7-KBBE-2013-7-613549-INDOX, H2020-BBI-PPP-2015-2-720297-ENZOX2, the COST-Action CM1303, and the Spanish Government projects BIO2013-43407-R-DEWRY and BIO2016-79106-R-LIGNOLUTION.

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Authors and Affiliations

  1. Department of Biocatalysis, Institute of Catalysis, Consejo Superior de Investigaciones Científicas (CSIC), 28049, Madrid, Spain

    Patricia Molina-Espeja, Patricia Gómez de Santos & Miguel Alcalde

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  1. Patricia Molina-Espeja
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  2. Patricia Gómez de Santos
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  3. Miguel Alcalde
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Correspondence to Miguel Alcalde .

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  1. Department of Biocatalysis, Institute of Catalysis and Petrochemistry, CSIC, Madrid, Spain

    Miguel Alcalde

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Molina-Espeja, P., de Santos, P.G., Alcalde, M. (2017). Directed Evolution of Unspecific Peroxygenase. In: Alcalde, M. (eds) Directed Enzyme Evolution: Advances and Applications. Springer, Cham. https://doi.org/10.1007/978-3-319-50413-1_5

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