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Production of Protein Complexes in Non-methylotrophic and Methylotrophic Yeasts

Nonmethylotrophic and Methylotrophic Yeasts

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Advanced Technologies for Protein Complex Production and Characterization

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 896))

Abstract

Protein complexes can be produced in multimilligram quantities using nonmethylotrophic and methylotrophic yeasts such as Saccharomyces cerevisiae and Komagataella (Pichia) pastoris. Yeasts have distinct advantages as hosts for recombinant protein production owing to their cost efficiency, ease of cultivation and genetic manipulation, fast growth rates, capacity to introduce post-translational modifications, and high protein productivity (yield) of correctly folded protein products. Despite those advantages, yeasts have surprisingly lagged behind other eukaryotic hosts in their use for the production of multisubunit complexes. As our knowledge of the metabolic and genomic bottlenecks that yeast microorganisms face when overexpressing foreign proteins expands, new possibilities emerge for successfully engineering yeasts as superb expression hosts. In this chapter, we describe the current state of the art and discuss future possibilities for the development of yeast-based systems for the production of protein complexes.

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Acknowledgments

This work was funded by the Spanish Ministry of Economy and Competitiveness grant CTQ2015-66206-C2-2-R and the European Commission (Framework Programme 7) (FP7) project ComplexINC No. 279039 to M.C.V.

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  1. Center for Biological Research, Spanish National Research Council (CIB-CSIC), Ramiro de Maeztu 9, 28040, Madrid, Spain

    Francisco J. Fernández, Miguel López-Estepa, Javier Querol-García & M. Cristina Vega

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    M. Cristina Vega

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Fernández, F.J., López-Estepa, M., Querol-García, J., Vega, M.C. (2016). Production of Protein Complexes in Non-methylotrophic and Methylotrophic Yeasts. In: Vega, M. (eds) Advanced Technologies for Protein Complex Production and Characterization. Advances in Experimental Medicine and Biology, vol 896. Springer, Cham. https://doi.org/10.1007/978-3-319-27216-0_9

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