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Analysis of heat shock promoters inHansenula polymorpha: TheTPS1 promoter, a novel element for heterologous gene expression

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Abstract

The strength and regulatory characteristics of the heat-inducibleHSA1, HSA2 andTPS1 promoters were compared with those of the well-established, carbon source-regulatedFMD promoter in aHansenula polymorpha-based host systemin vivo. In addition, theSaccharomyces cerevisiae-derivedADH1 promoter was analysed. WhileADH1 promoter showed to be of poor activity in the foreign host, the strength of the heat shockTPS1 promoter was found to exceed that of theFMD promoter, which at present is considered to be the strongest promoter for driving heterologous gene expression inH. polymorpha.

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Authors and Affiliations

  1. Institut für Mikrobiologie, Heinrich-Heine-Universität, Universitätsstr. 1, 40225, Düsseldorf, Germany

    Carsten Amuel & Cornelis P. Hollenberg

  2. Rhein Biotech GmbH, Eichsfelder Str. 11, 40595, Düsseldorf, Germany

    Gerd Gellissen & Manfred Suckow

Authors
  1. Carsten Amuel
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  2. Gerd Gellissen
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  3. Cornelis P. Hollenberg
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  4. Manfred Suckow
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Correspondence to Gerd Gellissen.

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Amuel, C., Gellissen, G., Hollenberg, C.P. et al. Analysis of heat shock promoters inHansenula polymorpha: TheTPS1 promoter, a novel element for heterologous gene expression. Biotechnol. Bioprocess Eng. 5, 247–252 (2000). https://doi.org/10.1007/BF02942181

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  • DOI: https://doi.org/10.1007/BF02942181

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