Skip to main content
SpringerLink
Log in

Degradation of Anthroquinone Dyes Stimulated by Fungi

  • Chapter
  • First Online:
Microbial Degradation of Synthetic Dyes in Wastewaters

Part of the book series: Environmental Science and Engineering ((ENVSCIENCE))

Abstract

Synthetic dyes are widely used in textile dyeing, paper printing, color photography, pharmaceuticals, food, leather industries, and cosmetics (Couto, Biotechnol Adv 27:227–235, 2009). Annual production of synthetic dyes has already exceeded over 7 × 105 metric tons (Chen et al., J Biotechnol 101:57–68, 2003). Different industries, such as paper and pulp mills, textiles and dyestuff industries, distilleries and tanneries, discharge an enormous amount of highly colored waste water (Raghukumar, Mycol Res 104:1222–1226, 2000). Among the industries, the textile industry generates maximum liquid effluent, as a large quantity of water is used in the dyeing processes (Kalyani et al., J Hazard Mater 163:735–742, 2009).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
eBook
USD 16.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Abadulla E, Tzanov T, Costa S, Robra KH, Cavaco-Paulo A, Gubitz GM (2000) Decolorization and detoxification of textile dyes with a laccase from Trametes hirsuta. Appl Environ Microbiol 66(8):3357–3362

    Article  Google Scholar 

  • Andleeb S, Atiq N, Robson GD, Ahmed S (2012) An investigation of anthraquinone dye biodegradation by immobilized Aspergillus flavus in fluidized bed bioreactor. Environ Sci Pollut Res 19:1728–1737

    Article  Google Scholar 

  • Annuar MSM, Adnan S, Vikineswary S, Chisti Y (2009) Kinetics and energitics of azo dye decolorization by Pycnoporus sanguineus. Water Air Soil Pollut 202:179–188

    Article  Google Scholar 

  • Aust SD (1995) Mechanisms of degradation by white rot fungi. Environ Health Pers 103:59–61

    Article  Google Scholar 

  • Baldrian P (2006) Fungal laccases—occurrence and properties. FEMS Microbiol Rev 30:215–242

    Article  Google Scholar 

  • Banat IM, Nigam P, Singh D, Marchant R (1996) Microbial decolorization of textile-dye-containing effluents. Bioresour Technol 58:217–227

    Article  Google Scholar 

  • Banci L (1997) Structural properties of peroxidases. J Biotechnol 53(2–3):253–263

    Article  Google Scholar 

  • BenYounes S, Mechichi T, Sayadi S (2007) Purification and characterization of the laccase secreted by the white rot fungus Perenniporia tephropora and its role in the decolourization of synthetic dyes. J Appl Microbiol 102(4):1033–1042

    Google Scholar 

  • Cai D, Tien M (1992) Kinetic studies on the formation and decomposition of compound II and III. Reactions of lignin peroxidase with H2O2. J Biol Chem 267:11149–11155

    Google Scholar 

  • Cai D, Tien M (1993) Lignin-degrading peroxidases of Phanerochaete chrysosporium. J Biotechnol 30:79–90

    Article  Google Scholar 

  • Camarero S, Ibarra D, Martınez MJ, Martınez AT (2005) Lignin-derived compounds as efficient laccase mediators for decolorization of different types of recalcitrant dyes. Appl Environ Microbiol 71:1775–1784

    Article  Google Scholar 

  • Cartoni A, Menna P, Salvatorelli E, Braghiroli D, Giampietro R, Animati F, Urabani A, Boccio PD, Minotti G (2004) Oxidative degradation of cardiotoxic anticancer anthracyclines to phthalic acids. J Biol Chem 279:5088–5099

    Article  Google Scholar 

  • Casieri L, Varese GC, Anatasi A, Prigione V, Svobodova K, Marchisio VF, Novotny C (2008) Decolorization and Detoxification of reactive industrial dyes by immobilized fungi Trametes pubescens and Pleurotus ostreatus. Folia Microbiol 53(1):44–52

    Article  Google Scholar 

  • Champagne PP, Ramsay JA (2005) Contribution of manganese peroxidase and laccase to dye decolourization by Trametes versicolor. Appl Microbiol Biotechnol 69:276–285

    Article  Google Scholar 

  • Chen KC, Wu JY, Liou DJ, Huang SCJ (2003) Decolorization of the textile dyes by newly isolated bacterial strains. J Biotechnol 101:57–68

    Article  Google Scholar 

  • Couto RS, Herrera JT (2006) Industrial biotechnological applications of laccases: a review. Biotechnol Adv 24:500–513

    Article  Google Scholar 

  • Couto SR (2009) Dye removal by immobilized fungi. Biotechnol Adv 27:227–235

    Article  Google Scholar 

  • Duarte-Vazquez MA, Whitaker JR, Rojo-Dominguez A, Garcia- Almendarez BE, Regalado C (2003) Isolation and thermal characterization of an acidic isoperoxidase from turnip roots. J Agri Food Chem 51(17):5096–5102

    Google Scholar 

  • Erkurt A, Unyayar EA, Kumbur H (2007) Decolorization of synthetic dyes by white rot fungi, involving laccase enzyme in the process. Process Biochem 42(10):1429–1435

    Article  Google Scholar 

  • Ezeronye OU, Okerentugba PO (1999) Performance and efficiency of a yeast biofilter for the treatment of a Nigerian fertilizer plant effluent. World J Microbiol Biotechnol 15:515–516

    Article  Google Scholar 

  • Fernaud Hernández JR, Carnicero A, Perestelo F, Hernández Cutuli M, Arias E, Falcón MA (2006) Upgrading of an industrial lignin by using laccase produced by Fusarium proliferatum and different laccase-mediator systems. Enzyme Microbiol Technol 38:40–48

    Google Scholar 

  • Field JA, De Jong E, Feijoo G, De Bont JAM (1993) Screening for ligninolytic fungi applicable to the biodegradation of xenobiotics. Trends Biotechnol 11:44–49

    Article  Google Scholar 

  • Forootanfar H, Moezzi A, Aghaie-Khozani M, Mahmoudjanlou Y, Ameri A, Niknejad F, Faramarzi MA (2012) Synthetic dye decolorization by three sources of fungal laccase. Iranian J Environ Health Sci Eng 9:27

    Article  Google Scholar 

  • Fu Y, Viraraghavan T (2001) Fungal decolorization of dye wastewaters: a review. Biores Technol 79:251–262

    Article  Google Scholar 

  • Giardina P, Faraco V, Pezzella C, Piscitelli A, Vanhulle S, Sannia G (2010) Laccases: a never-ending story. Cell Mol Life Sci 67:369–385

    Article  Google Scholar 

  • Giffhorn F (2000) Fungal pyranose oxidases: Occurrence, properties and biotechnical applications in carbohydrate chemistry. Appl Microbiol Biotechnol 54(6):727–740

    Article  Google Scholar 

  • Glenn JK, Alileswaran L, Gold MH (1986) Mn(II) oxidation is the principal function of the extracellular Mn-peroxidase from Phanerochaete chrysosporium. Arch Biochem Biophys 251:688–696

    Article  Google Scholar 

  • Glenn JK, Gold MH (1985) Purification and characterization of an extracellular Mn (II)-dependent peroxidase from the lignin-degrading basidiomycete, Phanerochaete chrysosporium. Arch Biochem Biophys 242:329–341

    Article  Google Scholar 

  • Gurav AA, Ghosh JS, Kulkarni GS (2011) Decolorization of anthroquinone based dye Vat Red 10 by Pseudomonas desmolyticum NCIM 2112 and Galactomyces geotrichum MTCC 1360. Int J Biotechnol Mole Biol Res 2(6):93–97

    Google Scholar 

  • Hadibarata T, Yusoff ARM, Kristanti RA (2011) Decolorization and metabolism of anthraquionone-type dye by laccase of white-rot fungi Polyporus sp. S133. Water Air Soil Pollut. doi:10.1007/s11270-011-0914-6

  • Hofrichter M (2002) Review: lignin conversion by manganese peroxidase (MnP). Enzyme Microb Technol 30(4):454–466

    Article  Google Scholar 

  • Hsu CA, Wen TN, Su YC, Jiang ZB, Chen CW, Shyur LF (2012) Biological degradation of anthroquinone and azo dyes by a novel laccase from Lentinus sp. Environ Sci Technol 46:5109–5117

    Article  Google Scholar 

  • Itoh K, Kitade C, Yatome C (1996) A pathway for biodegradation of ananthraquinone dye, C.I disperse red 15, by a yeast strain Pichia anomala. Bull Environ Contam Toxicol 56(3):413–418

    Article  Google Scholar 

  • Jin X, Liu G, Xu Z, Yao W (2007) Decolorization of a dye industry effluent by Aspergillus fumigatus XC6. Appl Microbiol Biotechnol 74:239–243

    Article  Google Scholar 

  • Joshi M, Bansal R, Purwar R (2004) Color removal from textile effluents. Indian J Fibre Textile Res 29:239–259

    Google Scholar 

  • Kalyani DC, Telke AA, Dhanve RS, Jadhav JP (2009) Ecofriendly biodegradation and detoxification of Reactive Red 2 textile dye by newly isolated Pseudomonas sp. SUK1. J Hazard Mater 163:735–742

    Article  Google Scholar 

  • Kersten PJ, Tien M, Kalyanaraman B, Kirk TK (1985) The ligninase of Phanerochaete chrysosporium generates cation radicals from methoxybenzenes. J Biol Chem 260:2609–2612

    Google Scholar 

  • Kiiskinen LL, Viikari L, Kruus K (2002) Purification and characterization of a novel laccase from the ascomycete Melanocarpus albomyces. Appl Microbiol Biotechnol 59:198–204

    Article  Google Scholar 

  • Kim SJ, Shoda M (1999) Purification and characterization of a novel peroxidase from Geotricum candidum Dec 1 involved in decolorization of dyes. Appl Environ Microbiol 65:1029–1035

    Google Scholar 

  • Knapp JS, Newby PS, Reace LP (1995) Decolourization of dyes by wood rotting basidiomycete basic fungi. Enzyme Microbial Technol 17:664–668

    Article  Google Scholar 

  • Knapp JS, Vantoch-Wood EJ, Zhang F (2001) Use of wood—rotting fungi for the decolorization of dyes and industrial effluents. In: Gadd GM (ed) Fungi in bioremediation. Cambridge University Press, Cambridge, pp 253–261

    Google Scholar 

  • Korniollowicz-Kowalska T, Rybczynska K (2014) Anthraquinone dyes decolorization capacity of anamorphic Bjerkandera adusta CCBAS 930 strain and its HRP-like negative mutants. World J Microbiol Biotechnol. doi:10.1007/s11274-014-1595-2

    Google Scholar 

  • Kuan IC, Tien M (1993) Stimulation of Mn peroxidase activity: A possible role for oxalate in lignin biodegradation. Proc Nat Acad Sci USA 90:1242–1246

    Article  Google Scholar 

  • Leonowicz A, Cho NS, Luterek J, Wilkolazka A, Wojtas-Wasilewska M, Matuszewska A, Hofrichter M, Wesenberg D, Rogalski J (2001) Fungal laccase: properties and activity on lignin. J Basic Microbiol 41(3–4):185–227

    Article  Google Scholar 

  • Levin L, Diorio L, Grassi E, Forchiassin F (2012) Grape stalks as substrate for white rot fungi, lignocellulolytic enzyme production and dye decolorization. Revista Argentina de Microbiología 44:105–112

    Google Scholar 

  • Majeau JA, Brar SK, Tyagi RD (2010) Laccases for removal of recalcitrant and emerging pollutants. Biores Technol 101:2331–2350

    Article  Google Scholar 

  • Martinez AT (2002) Molecular biology and structure-function of lignin-degrading heme peroxidases. Enzyme Microbial Technol 30:425–444

    Article  Google Scholar 

  • McMullan G, Meehan C, Conneely A, Kirby N, Robinson T, Nigam P, Banat IM, Marchant R, Smyth WF (2001) Microbial decolourisation and degradation of textile dyes. Appl Microbiol Biotechnol 56:81–87

    Article  Google Scholar 

  • Mester T, Tien M (2000) Oxidation mechanism of ligninolytic enzymes involved in the degradation of environmental pollutants. Int Biodeterio Biodegrad 46:51–59

    Article  Google Scholar 

  • Michniewicz A, Ledakowicz S, Ullrich R, Hofrichter M (2008) Kinetics of the enzymatic decolorization of textile dyes by laccase from Cerrena unicolor. Dyes Pigm 77:295–302

    Article  Google Scholar 

  • Mishra A, Kumar S (2009) Kinetic studies of laccase enzyme of Coriolus versicolor MTCC 138 in an inexpensive culture medium. J Biochem Eng 46:252–256

    Article  Google Scholar 

  • Moreira MT, Feijoo G, Sierra-Alvarez R, Lema J, Field JA (1997) Biobleaching of oxygen delignified kraft pulp by several white rot fungal strains. J Biotechnol 53:237–251

    Article  Google Scholar 

  • Murugesan K, Arulmani M, Nam IH, Kim YM, Chang YS, Kalaichelvan PT (2006) Purification and characterization of laccase produced by a white rot fungus Pleurotus sajor-caju under submerged culture condition and its potential in decolorization of azo dyes. Appl Microbiol Biotechnol 72:939–946

    Article  Google Scholar 

  • Ngieng NS, Zulkharnain A, Roslan HA, Husaini A (2013) Decolourisation of synthetic dyes by endophytic fungal flora isolated from Senduduk plant (Melastoma malabathricum). Hindawi Publishing Corporation. doi:10.5402/2013/260730

  • O’Neill C, Hawkes FR, Hawkes DL, Lourenco ND, Pinheiro HM, Delee W (1999) Color in textile effluents sources, measurement, discharge consents and simulation: a review. J Chem Technol Biotechnol 74:1009–1018

    Article  Google Scholar 

  • Pandey A, Singh P, Iyengar L (2007) Bacterial decolorization and degradation of azo dyes. Int Biodeterio Biodegrad 59(2):73–84

    Article  Google Scholar 

  • Pavko A, Novotny C (2008) Induction of ligninolytic enzyme production by Dichomitus squalens on various types of immobilization support. Acta Chim Slov 55:648–652

    Google Scholar 

  • Pointing SB (2001) Feasibility of bioremediation by white-rot fungi. Appl Microbiol Biotechnol 57:20–33

    Article  Google Scholar 

  • Poulos TL, Kraut J (1980) The stereochemistry of peroxidase catalysis. J Biol Chem 255:8199–8205

    Google Scholar 

  • Radha KV, Regupathi I, Arunagiri A, Murugesan T (2005) Decolorization studies of synthetic dyes using Phanerochaete chrysosporium and their kinetics. Process Biochem 40(10):3337–3345

    Google Scholar 

  • Raghukumar C (2000) Fungi from marine habitats: an application in bioremediation. Mycol Res 104:1222–1226

    Article  Google Scholar 

  • Reszka KJ, Wagner BA, Teesch LM, Britigan BE, Spitz DR, Burns CP (2005) Inactivation of anthracyclines by cellular peroxidase. Cancer Res 65:6346–6353

    Article  Google Scholar 

  • Robinson T, McMullan G, Marchant R, Nigam P (2001) Remediation of dyes in textile effluent: A critical review on current treatment technologies with a proposed alternative. Bioresour Technol 77:247–255

    Article  Google Scholar 

  • Rodriguez-Couto S (2007) Decolouration of industrial azo dye by crude laccase from Trametes hirsuta. J Hazard Mater 148:768–770

    Article  Google Scholar 

  • Saratale GD, Bhosale SK, Kalme SD, Govindwar SP (2007) Biodegradation of kerosene in Aspergillus ochraceus (NCIM-1146). J Basic Microbiol 47:400–405

    Article  Google Scholar 

  • Sarkar S, Martinez AT, Martinez MJ (1997) Biochemical and molecular characterization of a manganese peroxidase isoenzyme from Pleurotus ostreatus. Biochim Biophys Acta 1339:23–30

    Article  Google Scholar 

  • Schliephaje K, Lonergan GT (1996) Laccase variation during dye decolorisation in a 200 L packed-bad bioreactor. Biotechnol Lett 18:881–886

    Article  Google Scholar 

  • Schliephake K, Mainwaring DE, Lonergan GT, Jones IK, Baker WL (2000) Transformation and degradation of the disazodye Chicago Sky Blue by a purified laccase from Pycnoporus cinnabarinus. Enzyme Microb Technol 27:100–107

    Article  Google Scholar 

  • Shakeri M, Sugano Y, Shoda M (2007) Production of dye decolorizing peroxidase (rDyP) from complex substrates by repeated-batch and Fed-batch cultures of recombinant Aspergillus oryzae. J Biosci Bioeng 103:129–134

    Article  Google Scholar 

  • Sharma DK, Saini HS, Chimni SS, Chadha BS (2004a) Biological treatment of textile dye acid violet-17 by bacterial consortium in an up-flow immobilized cell bioreactor. Lett Appl Microbiol 38:345–350

    Article  Google Scholar 

  • Sharma DK, Saini HS, Singh M, Chimni SS, Chadha BS (2004b) Biodegradation of acid blue-15, a textile dye, by an up-flow immobilized cell bioreactor. J Ind Microbiol Biotechnol 31:109–114

    Article  Google Scholar 

  • Sharma DK, Saini HS, Singh M, Chimni SS, Chadha BS (2004c) Biotreatment of simulated textile dye effluent containing malachite green by an up-flow immobilized cell bioreactor. World J Microbiol Biotechnol 20:431–434

    Article  Google Scholar 

  • Shedbalkar U, Dhanve R, Jadhav J (2008) Biodegradation of triphenylmethane dye Cotton blue by Penicillium ochrochloron MTCC 517. J Hazard Mater 157:472–479

    Article  Google Scholar 

  • Siddiqui MF, Andleeb S, Ali N, Ghumro PB, Ahmed S (2010) Biotreatment of anthraquinone dye Drimarene Blue K2RL. African J Environ Sci Technol 4(1):045–050

    Google Scholar 

  • Singh H (2006) Mycoremediation-Fungal Bioremediation. Wiley Interscience, Hoboken, pp 421–471

    Google Scholar 

  • Soares GMB, De Amorim MTP, Costa-Ferreira M (2001) Use of laccase together with redox mediators to decolourize Remazol Brilliant Blue R. J Biotechnol 89(2–3):123–129

    Article  Google Scholar 

  • Strittmatter E, Liers C, Ullrich R, Wachter S, Hofrichter M, Plattner DA, Piontek K (2013) Transfer Sites and long-range electron peroxidase: substrate interaction High-redox Potential Dye-decolorizing. J Biol Chem 288(6):4095–4102

    Article  Google Scholar 

  • Sugano Y, Matsushima Y, Tsuchiya K, Aoki H, Hirai M, Shoda M (2009) Degradation pathway of an anthraquinone dye catalyzed by a unique peroxidase DyP from Thanatephorus cucumeris Dec. Biodegradation 20(3):433–440

    Article  Google Scholar 

  • Sugano Y, Nakano R, Sasaki K, Shoda M (2000) Efficient heterologous expression in Aspergillus oryzae of a unique dye-decolorizing peroxidase, DyP, of Geotrichum candidum Dec 1. Appl Environ Microbiol 66:1754–1758

    Article  Google Scholar 

  • Sugano Y, Matsushima Y, Shoda M (2006) Complete decolorization of the anthraquinone dye reactive blue 5 by the concerted action of two peroxidases from Thanatephorus cucumeris Dec1. Appl Microbiol Biotechnol 73(4):862–871

    Article  Google Scholar 

  • Swamy J, Ramsay J (1999a) Effects of glucose and nh +4 concentrations on sequential dye decoloration by Trametes versicolor. Enzyme Microb Technol 25(3-5):278-284

    Google Scholar 

  • Swamy J, Ramsay J (1999b) Effects of Mn2+ and NH4 + concentration on laccase and man-genese peroxidase production and Amaranth decoloration by Trametes versicolor. Appl Microbiol Biotechnol 51(3):391–396

    Article  Google Scholar 

  • Tien M, Kirk TK (1983) Lignin-degrading enzyme from the hymenomycete Phanerochaete chrysosporium burds. Science 221(4611):661–663

    Article  Google Scholar 

  • Tigini V, Prigione V, Donelli I, Anastasi A, Freddi G, Giansanti P, Mangiavillano A, Varese GC (2011) Cunninghamella elegans biomass optimisation for textile wastewater biosorption treatment: an analytical and ecotoxicological approach. Appl Microbiol Biotechnol 90:343–352

    Article  Google Scholar 

  • Torres E, Bustos-jaimes I, Borgne SL (2003) Potential use of oxidative enzymes for the detoxification of organic pollutants. Appl Cata B Environ 46:1–15

    Article  Google Scholar 

  • Verma AK, Raghukumar C, Parvatkar RR, Naik CG (2012) A rapid two-step bioremediation of the anthraquinone dye, reactive blue 4 by a marine-derived fungus. Water Air Soil Pollut 223(6):3499–3509

    Article  Google Scholar 

  • Wang XY, Yu J (1998) Adsorption and degradation of synthetic dyes on the mycelium of Trametese versicolor. Water Sci Technol 38:233–238

    Article  Google Scholar 

  • Wariishi H, Akileswaran L, Gold MH (1988) Manganese peroxidase from the basidiomycete Phanerochaete chrysosporium: spectral characterization of the oxidized states and the catalytic cycle. Biochemistry 27:5365–5370

    Article  Google Scholar 

  • Wariishi H, Valli K, Gold MH (1992) Manganese(II) oxidation by manganese peroxidase from the basidiomycete Phanerochaete chrysosporium. J Biol Chem 267:23688–23695

    Google Scholar 

  • Wesenberg D, Kyrikides I, Agathos SN (2003) White-rot fungi and their enzymes for the treatment of industrial dye effluents. Biotechnol Adv 22:161–187

    Article  Google Scholar 

  • Yang XQ, Zhao XX, Liu CY, Zheng Y, Qian SH (2009) Decolorization of azo, triphenylmethane and anthraquinone dyes by a newly isolated Trametes sp. SQ01 and its laccase. Process Biochem 44:1185–1189

    Article  Google Scholar 

  • Yoshida T, Tsuge H, Konno H, Hisabori T, Sugano Y (2011) The catalytic mechanism of dye-decolorizing peroxidase DyP may require the swinging movement of an aspartic acid residue. FEBS J 278:2387–2394

    Article  Google Scholar 

  • Zapanta LS, Tien M (1997) The roles of veratryl alcohol and oxalate in fungal lignin degradation. J Biotechnol 53:93–102

    Article  Google Scholar 

  • Zhang X, Liu Y, Yan K, Wu H (2007) Decolorization of anthraquinone -type dye by bilirubin oxidase-producing non-ligninolytic fungus Myrothecium sp. IMER1. J Biosci Bioeng 104(2):104–110

    Article  Google Scholar 

  • Zheng Z, Obbard JP (2002) Oxidation of polycyclic aromatic hydrocarbons (PAH) by the white rot fungus, Phanerochaete chrysosporium. Enzyme and Microbial Technology 31:3–9

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Plant Ecology and Environmental Science Division, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow, 226001, India

    S. N. Singh, Shweta Mishra & Nitanshi Jauhari

Authors
  1. S. N. Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shweta Mishra
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Nitanshi Jauhari
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to S. N. Singh .

Editor information

Editors and Affiliations

  1. Plant Ecology and Environmental Science Division, CSIR - National Botanical Research Institute, Lucknow, Uttar Pradesh, India

    Shree Nath Singh

Rights and permissions

Reprints and permissions

Copyright information

© 2015 Springer International Publishing Switzerland

About this chapter

Cite this chapter

Singh, S.N., Mishra, S., Jauhari, N. (2015). Degradation of Anthroquinone Dyes Stimulated by Fungi. In: Singh, S. (eds) Microbial Degradation of Synthetic Dyes in Wastewaters. Environmental Science and Engineering(). Springer, Cham. https://doi.org/10.1007/978-3-319-10942-8_15

Download citation

Publish with us

Policies and ethics

Search

Navigation