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Contribution of manganese peroxidase and laccase to dye decoloration by Trametes versicolor

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Abstract

During dye decoloration by Trametes versicolor ATCC 20869 in modified Kirk’s medium, manganese peroxidase (MnP) and laccase were produced, but not lignin peroxidase, cellobiose dehydrogenase or manganese-independent peroxidase. Purified MnP decolorized azo dyes [amaranth, reactive black 5 (RB5) and Cibacron brilliant yellow] in Mn2+-dependent reactions but did not decolorize an anthraquinone dye [Remazol brilliant blue R (RBBR)]. However, the purified laccase decolorized RBBR five to ten times faster than the azo dyes and the addition of a redox mediator, 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), did not alter decoloration rates. Amaranth and RB5 were decolorized the most rapidly by MnP since they have a hydroxyl group in an ortho position and a sulfonate group in the meta position relative to the azo bond. During a typical batch decoloration with the fungal culture, the ratio of laccase:MnP was 10:1 to 20:1 (based on enzyme activity) and increased to greater than 30:1 after decoloration was complete. Since MnP decolorized amaranth about 30 times more rapidly than laccase per unit of enzyme activity, MnP should have contributed more to decoloration than laccase in batch cultures.

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Acknowledgements

This research is financially supported by the Natural Science and Engineering Research Council of Canada, the Premier’s Research Excellence Award, Government of Ontario, and the Chancellor’s Award of Queen’s University.

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  1. Department of Chemical Engineering, Queen’s University, Kingston, ON, K7L 3N6, Canada

    Paul-Philippe Champagne & Juliana Akit Ramsay

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  1. Paul-Philippe Champagne
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  2. Juliana Akit Ramsay
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Correspondence to Juliana Akit Ramsay.

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Champagne, PP., Ramsay, J.A. Contribution of manganese peroxidase and laccase to dye decoloration by Trametes versicolor . Appl Microbiol Biotechnol 69, 276–285 (2005). https://doi.org/10.1007/s00253-005-1964-8

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  • DOI: https://doi.org/10.1007/s00253-005-1964-8

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