Skip to main content
SpringerLink
Log in

Anatomical Evidence for GABA-5 HT Interaction in Serotonergic Neurons

  • Chapter
Serotonin

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 133))

Abstract

Numerous studies have indicated possible metabolic interactions between GABA and monoamines 1,2,3,4,5,6,7,8. Recently, it was postulated that GABA could be responsible for inhibition of the firing of serotonin (5 HT) containing neurons of the nucleus raphe dorsalis (NRD) 9,10,11,12. Recently 13,14,15 we have demons-trated the presence of terminals, fibers and nerve cell bodies accumulating 3H GABA in the NRD. These elements were postulated to belong to a local GABAergic network since lesions of principal NRD afferents failed to produce important changes in glutamate decarboxylase (GAD) activity. Finally, such elements were observed in all the periventricular area and could correspond to the existence of a periventricular GABAergic system.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
eBook
USD 16.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Similar content being viewed by others

References

  1. W.J. Nickles, S. Berl and D.D. Clarke, Interaction of catecholamine and amino acid metabolism in brain: effect of pargyline and L-DOPA, J. Neurochem. 23: 149 (1974).

    Article  Google Scholar 

  2. C.J. Patel, R.P. Schatz, S.M. Constantinides and L.A.L. Harbans, Effects of desipramine and pargyline on brain gamma-aminobutyric acid, Biochem. Pharmacol. 24: 57 (1975).

    CAS  Google Scholar 

  3. N.H. Yessaian, A.H. Demirjian and B.V. Tozakalian, Effect of GABA, picrotoxin and bicuculline on loss of noradrenaline and serotonin from rat brain mesodiencephalic region in vitro, Biochem. Pharmacol. 28: 1151 (1976).

    Google Scholar 

  4. N.H. Yessaian, A.R. Armenian, E.K. Kazarova and H.Ch. Buniatian, On the involvement of inorganic ions in the effect of y-aminobutyric acid on brain serotonin and norepinephrine, J. Neurochem. 19: 307 (1971).

    Article  Google Scholar 

  5. N.E. Anden and G. Stock, Inhibitory effec of hydroxybutyric acid and aminobutyric acid on the dopamine cells in the substantia nigra, Naunyn-Schmiedeberg’s Arch. Exp. Path. Pharmak. 279: 89 (1973).

    CAS  Google Scholar 

  6. G. Bartholini and H. Stadler, Cholinergic and GABAergic influences on the dopamine release in extrapyramidal centers, in: “Chemical tools in catecholamine research” vol II, S. Almgren, A. Carlsson and J. Engel, ed., North-Holland Publishing Co, Amsterdam (1975).

    Google Scholar 

  7. A. Cheramy, A. Nieoullon and J. Glowinski, GABAergic processes involved in the control of dopamine release from nigrostriatal dopaminergic neuorns in the cat, Europ. J. Pharmacol. 48: 281 (1978).

    CAS  Google Scholar 

  8. P.M. Groves, C.J. Wilson, S.J. Young and G.V. Rebec, Self inhibition by dopaminergic neurons, Science 190: 522 (1975).

    Article  PubMed  CAS  Google Scholar 

  9. D.W. Gallager and G.K. Aghajanian, Effect of antipsychotic drugs on the firing of drosal raphe cells. I - Role of adrenergic system, Europ. J. Pharmacol. 39: 341 (1976).

    CAS  Google Scholar 

  10. D.W. Gallager and G.K. Aghajanian, Effect of antipsychotic drugs on the firing of dorsal raphe cells. II - Reversal by picrotoxin, Europ. J. Pharmacol. 39: 357 (1976).

    CAS  Google Scholar 

  11. R.Y. Wang, Physiological evidence for habenula as major link between forebrain and midbrain raphe, Science 197: 89 (1977).

    Article  PubMed  CAS  Google Scholar 

  12. R.Y. Wang, D.W. Gallager and G.W. Aghajanian, Stimulation of pontine reticular formation supresses firing of serotonergic neurones in the dorsal raphe, Nature 264: 365 (1976).

    Article  PubMed  CAS  Google Scholar 

  13. M.F. Belin, M. Aguera, M. Tappaz and J.F. Pujol, Identification of GABA accumulating neurons in the nucleus raphe dorsalis, C.R. Acad. Sci. 287: 865 (1978).

    CAS  Google Scholar 

  14. M.F. Belin, A. Aguera, M. Tappaz, A. McRae-Degueurce,P. Bobillier and J.F. Pujol, GABA-accumulating neurons in the nucleus raphe dorsalis and periaqueductal gray in the rat: a biochemical and radioautographic study, Brain Res. 170: 279 (1979).

    Article  PubMed  CAS  Google Scholar 

  15. H. Gamrani, A. Calas, M.F. Belin, M. Aguera and J.F. Pujol, High resolution radioautographic identification of 3H GABA labeled neurons in the rat raphe dorsalis, Neurosci. Let., in press.

    Google Scholar 

  16. M.W. Brightman and S.L. Palay, The fine structure of ependyma in the brain of the rat, J. Cell. Biol. 19: 415 (1963).

    Article  PubMed  CAS  Google Scholar 

  17. E. Westergaard, The fine structure of nerve fibers and endings in the lateral cerebral ventricles of the rat, J. Comp. Neurol. 144: 345 (1972).

    Article  PubMed  CAS  Google Scholar 

  18. A. Calas, 0. Bosler, M. Arluison and C. Bouchaud, Serotonin as a neurohormone in circumventricular organs and subependymal fibers, in: “Brain endocrine interaction. III - Neural hormones and reproduction, D.E. Scott, G.P. Kozlowski and A. Weindl, ed., S. Karger, Basel (1978).

    Google Scholar 

  19. G. Richards, H.P. Lorez and J.P. Tranzer, Indolealkylamine nerve terminals in cerebral ventricles: identification by electron microscopy and fluorescence histochemistry, Brain Res. 57: 277 (1973).

    Article  PubMed  CAS  Google Scholar 

  20. G. Alonso, E Pons et J. Cadilhac, Mise en évidence par radio-autographie de terminaisons indolaminergiques dans les parois ventriculaires cérébrales chez le rat, C.R. Soc. Biol. 168: 1021 (1974).

    CAS  Google Scholar 

  21. H.P. Lorez and J.G. Richards, 5 HT nerve terminals in the fourth ventricle of the rat brain: their identification and distribution studied by fluorescence,histochemistry and electron microscopy, Cell. Tiss. Res. 165: 37 (1975).

    CAS  Google Scholar 

  22. J.G. Richards, Autoradiographic evidence for the selective accumulation of 3H 5 HT by supra-ependymal nerve terminals, Brain Res. 134: 151 (1977).

    Article  PubMed  CAS  Google Scholar 

  23. V. Chan-Palay, Serotonin axons in the supra-and sub-ependymal plexuses and in the leptomeninges; their roles in local alterations of cerebrospinal fluid and vasomotor activity, Brain Res. 102: 103 (1976).

    Article  PubMed  CAS  Google Scholar 

  24. L. Descarries, A. Beaudet and K.C. Watkins, Serotonin nerve terminals in adult rat neoccrtex, Brain Res. 100: 563 (1975).

    Article  PubMed  CAS  Google Scholar 

  25. F. Larra et B. Droz, Techniques radioautographiques et leur application à l’étude du renouvellement des constituants cellulaires, J. Microscopie 9: 845 (1970).

    CAS  Google Scholar 

  26. M.F. Belin, H. Gamrani, M. Aguera, A. Calas and J.F. Pujol, Selective uptake of GABA by rat supra and sub-ependymal nerve fibers. Histological and high resolution radioautographic studies, submitted to Neuroscience.

    Google Scholar 

  27. D.L. Martin and A.A. Smith, Ions and the transport of GABA by synaptosomes, J. Neurochem. 19: 841 (1972).

    Article  PubMed  CAS  Google Scholar 

  28. G. Levi and M. Raiteri, Exchange of neurotransmitter amino-acid at nerve endings can simulate high affinity uptake, Nature 250: 735 (1974).

    Article  PubMed  CAS  Google Scholar 

  29. F. Gonon, Mesure en continu du transport actif par les synaptosomes: application à l’étude du transport d’un neurotransmetteur (GABA), Thèse présentée à l’Université Claude Bernard, Lyon I (1979).

    Google Scholar 

  30. N. Halasz, A. Ljungdahl and T. Hokfelt, Transmitter histochemistry of the rat olfactory bulb. III - Autoradiographic localisation of 3H GABA, Brain Res. 167: 221 (1979).

    Article  PubMed  CAS  Google Scholar 

  31. V. Chan-Palay, Autoradiography localisation of y-aminobutyric acid receptors in the rat central nervous system using 3H muscimol, Proc. Nat. Acad. Sci. (U.S.A.) 75: 1024 (1978).

    Article  CAS  Google Scholar 

  32. N.G. Bowery, G.P. Jones and M.J. Neal, Selective inhibition of neuronal GABA uptake by Cis-1,3-aminocyclohexane carboxylic acid, Nature 264: 281 (1976).

    Article  CAS  Google Scholar 

  33. J.S. Kelly and P. Dick, Differential labelling of glial cells and GABA-inhibitory interneurons and nerve terminals following the microinjection of 3H $Alanine, 3H DABA and 3H GABA into single folia of the cerebellum, in “Cold Spring Habor Symposia on quantitative biology, vol 10 The synapse (1976).

    Google Scholar 

  34. W. Noack, L. Dumitrescu and J.U. Schweichel, Scanning and electron microscopical investigations of the surface structures of the lateral ventricles in the cat, Brain Res. 46: 121 (1972).

    Article  PubMed  CAS  Google Scholar 

  35. J.E. Bruni, D.G. Montemurro, R.E. Clattenburg and R.P. Singh, Scanning electron microscopy of the ependymal surface of the third ventricle after silver nitrate staining, Brain Res. 61: 207 (1973).

    Article  PubMed  CAS  Google Scholar 

  36. W.K. Paull, H. Martin and D.E. Scott, Scanning electron microscopy of the third ventricular floor of the rat, J. Comp. Neurol. 175: 301 (1974).

    Article  Google Scholar 

  37. R. Bleier, Surface fine structure of supra-ependymal elements and ependyma of hypothalamic third ventricle of mouse, J. Comp. Neurol. 161: 555 (1975).

    Article  PubMed  CAS  Google Scholar 

  38. A. Mitro, Light microscopy and histochemical study of the ependyma of the third cerebral ventricle in the albino rat, Folia Morphol. 23: 347 (1975).

    CAS  Google Scholar 

  39. A. Nitro and A. Kiss, The ependyma of the ventriculus mesencephali in the rat, Acta Morphol. Acad. Sci. Hung. 25: 1 (1977).

    Google Scholar 

  40. R.N.J. Cupedo, The surface ultrastructure of the habenular complex of the rat, Anat. Embryol. 152: 43 (1977).

    Article  CAS  Google Scholar 

  41. T. Yamamori, The directions of ciliary beat on the wall of the fourth ventricle in the mouse, Arch. Histol. Jap. 40: 283 (1977).

    Article  Google Scholar 

  42. A. Kiss and A. Mitro, Ependyma and supraependymal structures in some areas of the fourth ventricle in the rat, Acta Anat. 100: 521 (1978).

    Article  PubMed  CAS  Google Scholar 

  43. G.K. Aghajanian and D.W. Gallager, Raphe origin of serotonergic nerves terminating in the cerebral ventricles, Brain Res. 88: 221 (1975).

    Article  PubMed  CAS  Google Scholar 

  44. H.P. Lorez, L. Pieri and J.G. Richards, Disappearance of supraependymal 5 HT axons in the rat forebrain after electrolytic and 5,6-DHT-induced lesions of the medial forebrain bundle, Brain Res. 100: 1 (1975).

    Article  PubMed  CAS  Google Scholar 

  45. H.P. Lorez and J.G. Richards, Effects of intra cerebroventricular injection of 5,6-dihydroxytryptamine and 6-hydroxydopamine on supra-ependymal nerves, Brain Res. 116: 165 (1976).

    Article  PubMed  CAS  Google Scholar 

  46. V. Chan-Palay, Indoleamine neurons and their processes in’the normal rat brain and in chronic diet-induced thiamine deficiency demonstrated by uptake of 3H-serotonin, J. Comp. Neurol. 176: 467 (1977).

    Article  PubMed  CAS  Google Scholar 

  47. L.L. Iversen and F.E. Bloom, Studies if the uptake of 3H GABA and 3H glycine in slices and homogenates of rat brain and spinal cord by electron microscopic autoradiography, Brain Res. 41: 131 (1972).

    Article  PubMed  CAS  Google Scholar 

  48. T. Hattori, P.L. McGeer, H.C. Fibiger and E.G. McGeer, On the source of GABA-containing terminals in the substantia nigra. Electron microscopic autoradiographic and biochemical studies, Brain Res. 54: 103 (1973).

    Article  PubMed  CAS  Google Scholar 

  49. C. Sotelo, Radioautography as a tool for the study of putative neurotransmitters in the nervous system, J. Neur. Trans. suppl. XII: 75 (1975).

    Google Scholar 

  50. T. Hökfelt and A Ljungdhal, Autoradiographic identification of cerebral and cerebellar cortical neurones accumulating labelled gamma-aminobutyric acid (3H GABA), Exp. Brain Res. 14: 354 (1972).

    Article  PubMed  Google Scholar 

  51. R.W. Burry and R.S. Lasher, Electron microscopic autoradiography of the uptake of (3H) GABA in dispersed cell cultures of rat cerebellum. I - The morphology of the GABAergic synapse, Brain Res. 151: 1 (1978).

    Article  PubMed  CAS  Google Scholar 

  52. J.S. Kim, I.J. Bak, R. Hassler and Y. Okada, Role of y-aminobutyric acid (GABA) in the extra-pyramidal motor system. 2. Some evidence for the existence of a type of GABA-rich strionigral neurons, Exp. Brain Res. 14: 95 (1971).

    Article  PubMed  CAS  Google Scholar 

  53. J. Storm-Mathisen, GABA as a transmitter in the central nervous system of vertebrates, J. Neur. Trans. Suppl. XI: 227 (1974).

    Google Scholar 

  54. F. Schon and L.L. Iversen, The use of autoradiographic techniques for the identification and mapping of transmitter-specific neurones in the brain, Life Sci. 15: 157 (1974).

    Article  PubMed  CAS  Google Scholar 

  55. B.J. McLaughlin, J.G. Wood, K. Saito, R. Barber, J.E. Baughn, E. Roberts and J.Y. Wu, The fine structural localisation of glutamate decarboxylase in synaptic terminals of rodent cerebellum, Brain Res. 76: 377 (1974).

    Article  PubMed  CAS  Google Scholar 

  56. J. Storm-Mathisen, High affinity uptake of GABA in presumed GABAergic nerve endings in rat brain, Brain Res. 84: 409 (1975).

    Article  PubMed  CAS  Google Scholar 

  57. C.E. Ribak, J.E. Vaughn, K. Saito, R. Barber and E. Roberts, Immunocytochemical localization of glutamate decarboxylase in rats substantia nigra, Brain Res. 116: 287 (1976).

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. INSERM U 171, 8, Av. Rockefeller, 69008, Lyon, France

    Jean-François Pujol, Marie-Françoise Belin, Halima Gamrani, Michèle Aguera & André Calas

  2. CNRS, INP 08, 31, Chemin J. Aiguier, 13009, Marseille, France

    Jean-François Pujol, Marie-Françoise Belin, Halima Gamrani, Michèle Aguera & André Calas

Authors
  1. Jean-François Pujol
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Marie-Françoise Belin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Halima Gamrani
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Michèle Aguera
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. André Calas
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. The University of Texas Medical Branch, Galveston, Texas, USA

    Bernard Haber

  2. Veterans Administration Medical Center, Brockton, Massachusetts, USA

    Sabit Gabay

  3. Brown University Division of Psychiatry and Human Behavior, Providence, Rhode Island, USA

    Sabit Gabay

  4. University of Athens School of Medicine, Athens, Greece

    M. R. Issidorides  & S. G. A. Alivisatos  & 

Rights and permissions

Reprints and permissions

Copyright information

© 1981 Plenum Press, New York

About this chapter

Cite this chapter

Pujol, JF., Belin, MF., Gamrani, H., Aguera, M., Calas, A. (1981). Anatomical Evidence for GABA-5 HT Interaction in Serotonergic Neurons. In: Haber, B., Gabay, S., Issidorides, M.R., Alivisatos, S.G.A. (eds) Serotonin. Advances in Experimental Medicine and Biology, vol 133. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3860-4_4

Download citation

  • DOI: https://doi.org/10.1007/978-1-4684-3860-4_4

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-3862-8

  • Online ISBN: 978-1-4684-3860-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation