Abstract
We show that tetraphenylphosphonium inhibits oxidation of palmitoylcarnitine, pyruvate, malate, 2-oxoglutarate and glutamate in heart mitochondria in the range of concentration (1–5 µM) commonly used for the determination of mitochondrial membrane potential. The inhibition of 2-oxoglutarate (but not other substrate) oxidation by tetraphenylphosphonium is dependent on the concentration of 2-oxoglutarate and on extramitochondrial free calcium, and the kinetic plots are consistent with a mixed type of inhibition. Our results indicate that tetraphenylphosphonium interacts with enzymes, specifically involved in the oxidation of 2-oxoglutarate, most possibly, 2-oxoglutarate dehydrogenase. (Mol Cell Biochem 174: 67-70, 1997)
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Abbreviations
- TPP+ :
-
tetraphenylphosphonium
- TPMP+ :
-
triphenylmethylphosphonium
References
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© 1997 Springer Science+Business Media Dordrecht
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Mildaziene, V., Baniene, R., Marcinkeviciute, A., Nauciene, Z., Kalvenas, A., Zimkus, A. (1997). Tetraphenylphosphonium inhibits oxidation of physiological substrates in heart mitochondria. In: Gellerich, F.N., Zierz, S. (eds) Detection of Mitochondrial Diseases. Developments in Molecular and Cellular Biochemistry, vol 21. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-6111-8_10
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DOI: https://doi.org/10.1007/978-1-4615-6111-8_10
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