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Response of the rat heart to catecholamines and thyroid hormones

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Cellular Interactions in Cardiac Pathophysiology

Part of the book series: Developments in Molecular and Cellular Biochemistry ((DMCB,volume 14))

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Abstract

Catecholamines and thyroid hormones have a similar influence on heart function and metabolism, but this may occur in a differential manner and to a different extent. In this study, the effects of norepinephrine (NE) and of triiodothyronine (T3) were studied in regard to the function of the left (LV) and right ventricle (RV) and to the oxidative pentose phosphate pathway (PPP). NE was applied in rats as continuous i.v. infusion (0.2 mg/kg/h) for three days. T3 was given as daily s.c. injections (0.2 mg/kg) for the same period of time. LV and RV function was measured in the closed-chest trapanal-anesthetized animals using special Millar ultraminature catheter pressure transducers. NE induced an increase in heart rate, in mean arterial pressure, and in total peripheral resistance (TPR). The cardiac RNA/DNA and the left ventricular weight/body weight ratios were increased by about 40%. These effects were prevented by simultaneous α-and β-receptor blockade with prazosin and metoprolol, respectively, but not by verapamil which abolished the hemodynamic effects. RVSP was significantly elevated by NE in a dose-dependent manner. The functional effects of T3 on the LV were not as pronounced as those induced by NE. Heart rate and LV dp/dtmax were increased by T3, and this increase was prevented by concomitant β-receptor blockade with metoprolol. In contrast to NE, T3 induced an increase in cardiac output and a concomitant decrease in TPR. The RNA/DNA ratio was elevated and cardiac hypertrophy had developed after treatment for three days with T3. These changes were not affected by fl-receptor blockade with metoprolol. RVSP was increased by T3 to a lesser extent than with NE. In metabolic terms it turned out that only NE, but not T3 had a stimulating effect on the cardiac PPP. NE increased the mRNA and activity of glucose-6-phosphate dehydrogenase (G-6-PD), the first and regulating enzyme of this pathway. However, there was no effect of T3 on G-6-PD activity nor on 6-phosphogluconate dehydrogenase activity, one of the following enzymes in the pathway within the first 5 days of T3 treatment. These results demonstrate that the functional effects of T3 were not as pronounced as or even different from those of NE, and that T3 lacked a stimulating effect on the cardiac PPP. (Mol Cell Biochem 147: 105–114, 1995)

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References

  1. Sutherland EW, Robison GA, Butcher RW: Some aspects of the biological role of adenosine 3’,5’-monophosphate (cyclic AMP). Circulation 37: 279–306, 1968

    Article  CAS  Google Scholar 

  2. Tada M, Katz AM: Phosphorylation of the sarcoplasmatic reticulum and sarcolemma. Ann Rev Physiol 44: 401–423, 1982

    Article  CAS  Google Scholar 

  3. Rupp H, Berger H-J, Pfeifer A, Werdan K: Effect of positive inotropic agents on myosin isozyme population and mechanical activity of cultured rat heart myocytes. Circ Res 68: 1164–1173, 1991

    Article  PubMed  CAS  Google Scholar 

  4. Zierhut W, Zimmer H-G: Significance of myocardial a-and 3adrenoceptors in catecholamine-induced cardiac hypertrophy. Circ Res 65: 1417–1425, 1989

    Article  PubMed  CAS  Google Scholar 

  5. Berridge MJ, Irvine RF: Inositol trisphosphate, a novel second messenger in cellular signal transduction. Nature 312: 315–321, 1984

    Article  PubMed  CAS  Google Scholar 

  6. Nishizuka Y: The molecular heterogeneity of protein kinase C and its implications for cellular regulation. Nature 334: 661–665, 1988

    Article  PubMed  CAS  Google Scholar 

  7. Schmitz W, Scholz H, Scholz J, Steinfath M: Increase in IP3 precedes α1-adrenoceptor-induced increase in force of contraction in cardiac muscle. Eur J Pharmacol 140: 109–111, 1987

    Article  PubMed  CAS  Google Scholar 

  8. Kohl C, Schmitz W, Scholz H, Scholz J, Toth M, Döring V, Kalmar P: Evidence for α1-adrenoceptor-mediated increase of inositol trisphosphate in the human heart. J Cardiovasc Pharmacol 13: 324–327, 1989

    Article  PubMed  CAS  Google Scholar 

  9. Kolbeck-Rühmkorff C, Horban A, Zimmer H-G: Effect of pressure and volume overload on proto-oncogene expression in the isolated working rat heart. Cardiovasc Res 27: 1998–2004, 1993

    Article  PubMed  Google Scholar 

  10. Long CS, Ordahl CP, Simpson PC. α1-Adrenergic receptor stimulation of sarcomeric actin isogene transcription in hypertrophy of cultured rat heart muscle cells. J Clin Invest 83: 1078–1082, 1989

    Article  PubMed  CAS  Google Scholar 

  11. Lee HR, Henderson SA, Reynolds R, Dunnmon P, Yuan D, Chien KR: α1-Adrenergic stimulation of cardiac gene transcription in neonatal rat myocardial cells. Effects on myosin light chain-2-gene expression. J Biol Chem 263: 7352–7358, 1988

    PubMed  CAS  Google Scholar 

  12. Zimmer H-G, Lankat-Buttgereit B, Kolbeck-Rühmkorff C, Nagano T, Zierhut W: Effects of norepinephrine on the oxidative pentose phosphate pathway in the rat heart. Circ Res 71: 451–459, 1992

    Article  PubMed  CAS  Google Scholar 

  13. Oppenheimer JH: Thyroid hormone action at the cellular level. Science 203: 971–979, 1979

    Article  PubMed  CAS  Google Scholar 

  14. Weinberger C, Thompson CC, Ong ES, Lebo R, Gruol DJ, Evans RM: The c-erb-A gene encodes a thyroid hormone receptor. Nature 324: 641–646, 1986

    Article  PubMed  CAS  Google Scholar 

  15. Martial JA, Baxter JD, Goodman HM, Seeburg PH: Regulation of growth hormone messenger RNA by thyroid and glucocorticoid hormones. Proc Natl Acad Sci USA 74: 1816–1820, 1977

    Article  PubMed  CAS  Google Scholar 

  16. Williams LT, Lefkowitz RJ, Watanabe AM, Hathaway DR, Besch HR: Thyroid hormone regulation of β-adrenergic receptor number. J Biol Chem 252: 2787–2789, 1977

    PubMed  CAS  Google Scholar 

  17. Ismail-Beigi F, Bissell DM, Edelman IS: Thyroid thermogenesis in adult rat hepatocytes in primary monolayer culture. Direct action of thyroid hormone in vitro. J Gen Physiol 73: 369–383, 1979

    Article  PubMed  CAS  Google Scholar 

  18. Bartolome J, Huguenard J, Slotkin TA: Role of ornithine decarboxylase in cardiac growth and hypertrophy. Science 210: 793–794, 1980

    Article  PubMed  CAS  Google Scholar 

  19. Pegg AE, Hibasami H: Polyamine metabolism during cardiac hypertrophy. Am J Physiol 239: E372–E378, 1980

    PubMed  CAS  Google Scholar 

  20. Schimmelpfennig K, Sauerberg M, Neubert D: Stimulation of mitochondria] RNA synthesis by thyroid hormone. FEBS Letters 10: 269–272, 1970

    Article  PubMed  CAS  Google Scholar 

  21. Nelson BD, Joste V, Wielburski A, Rosenqvist U: The effects of triiodothyronine on the synthesis of mitochondrial proteins in isolated rat hepatocytes. Biochim Biophys Acta 608: 422–426, 1980

    Article  PubMed  CAS  Google Scholar 

  22. Seymour A-ML, Eldar H, Radda GK: Hyperthyroidism results in increased glycolytic capacity in the rat heart. A 31P-NMR study. Biochim Biophys Acta 1055: 107–116,1990

    Article  PubMed  CAS  Google Scholar 

  23. Tanaka T, Morita H, Koide H, Kawamura K, Takatsu T: Biochemical and morphological study of cardiac hypertrophy. Effects of thyroxine on enzyme activities in the rat. Basic Res Cardiol 80: 165–174, 1985

    Article  PubMed  CAS  Google Scholar 

  24. Hoh JFY, McGrath PA, Hale PT: Electrophoretic analysis of multiple forms of rat cardiac myosin: effects of hypophysectomy and thyroid replacement. J Mol Cell Cardiol 10: 1053–1076, 1977

    Article  Google Scholar 

  25. Hoh JFY, Egerton LJ: Action of triiodothyronine on the synthesis of rat ventricular myosin isoenzymes. FEBS Letters 101: 143–148, 1979

    Article  PubMed  CAS  Google Scholar 

  26. Chizzonite RA, Zak R: Regulation of myosin isoenzyme composition in fetal and neonatal rat ventricle by endogenous thyroid hormones. J Biol Chem 259: 12628–12632, 1984

    PubMed  CAS  Google Scholar 

  27. Gustafson TA, Markham BE, Morkin E: Effects of thyroid hormone on a-actin and myosin heavy chain gene expression in cardiac and skeletal muscle of the rat: measurement of mRNA content using synthetic oligonucleotide probes. Circ Res 59: 194–201, 1986

    Article  PubMed  CAS  Google Scholar 

  28. Zimmer H-G: Measurement of left ventricular hemodynamic parameters in closed-chest rats under control and various pathophysiological conditions. Basic Res Cardiol 78: 77–84, 1983

    Article  PubMed  CAS  Google Scholar 

  29. Zimmer H-G, Zierhut W, Seesko RC, Varekamp AE: Right heart catheterization in rats with pulmonary hypertension and right ventricular hypertrophy. Basic Res Cardiol 83: 48–57, 1988

    Article  PubMed  CAS  Google Scholar 

  30. Zimmer H-G: The oxidative pentose phosphate pathway in the heart: Regulation, physiological significance, and clinical implications. Basic Res Cardiol 87: 303–316, 1992

    Article  PubMed  CAS  Google Scholar 

  31. Zimmer H-G, Ibel H: Effects of ribose on cardiac metabolism and function in isoproterenol-treated rats. Am J Physiol 245: H880–H886, 1983

    PubMed  CAS  Google Scholar 

  32. Zimmer H-G, Ibel H, Steinkopff G, Korb G: Reduction of the isoproterenol-induced alterations in cardiac adenine nucleotides and morphology by ribose. Science 207: 319–321, 1980

    Article  PubMed  CAS  Google Scholar 

  33. Zimmer H-G, Ibel H: Ribose accelerates the repletion of the ATP pool during recovery from reversible ischemia of the rat myocardium. J Mol Cell Cardiol 16: 863–866, 1984

    Article  PubMed  CAS  Google Scholar 

  34. Zimmer H-G: Normalization of depressed heart function in rats by ribose. Science 220: 81–82, 1983

    Article  PubMed  CAS  Google Scholar 

  35. Zimmer H-G, Martius PA, Marschner G: Myocardial infarction in rats: effects of metabolic and pharmacologic interventions. Basic Res Cardiol 84: 332–343, 1989

    Article  PubMed  CAS  Google Scholar 

  36. Zimmer H-G, Ibel H, Suchner U: 3-Adrenergic agonists stimulate the oxidative pentose phosphate pathway in the rat heart. Circ Res 67: 1525–1534, 1990

    Article  PubMed  CAS  Google Scholar 

  37. Glock GE, McLean P: Further studies on the properties and assay of glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase of rat liver. Biochem J 55: 400–408, 1953

    PubMed  CAS  Google Scholar 

  38. Glock GE, McLean P: Levels of enzymes of the direct oxidative pathway of carbohydrate metabolism in mammalian tissues and tumours. Biochem J 56: 171–175, 1954

    PubMed  CAS  Google Scholar 

  39. Robinson HW, Hodgen CG: The biuret reaction in the determination of serum proteins. J Biol Chem 135: 727–731, 1940

    CAS  Google Scholar 

  40. Birnboim HC: Rapid extraction of high molecular weight RNA from cultured cells and granulocytes for Northern analysis. Nucleic Acids Res 16: 1487–1497, 1988

    Article  PubMed  CAS  Google Scholar 

  41. Maniatis T, Fritsch EF, Sambrook J: Molecular Cloning. Cold Spring Harbor, NY. Cold Spring Harbor Laboratory, 1982

    Google Scholar 

  42. Wallenstein S, Zucker CL, Fleiss JL: Some statistical methods useful in circulation research. Circ Res 47: 1–9, 1980

    Article  PubMed  CAS  Google Scholar 

  43. Zierhut W, Zimmer H-G: Differential effects of triiodothyronine on rat left and right ventricular function and the influence of metoprolol. J Mol Cell Cardiol 21: 617–6624, 1989

    Article  PubMed  CAS  Google Scholar 

  44. Rona G, Chappell CI, Kahn DS: The significance of factors modifying the development of isoproterenol-induced myocardial necrosis. Am Heart J 66: 389–395, 1963

    Article  PubMed  CAS  Google Scholar 

  45. Gerdes AM, Moore JA, Bishop SP: Failure of propranolol to prevent chronic hyperthyroid induces hypertrophy and multifocal cellular necrosis in the rat. Can J Cardiol I: 340–345, 1985

    PubMed  CAS  Google Scholar 

  46. Van Liere EJ, Sizemore DA, Hunnel J: Size of cardiac ventricles in experimental hyperthyroidism in the rat. Proc Soc Exp Biol Med 132: 663–665, 1969

    PubMed  Google Scholar 

  47. Gerdes AM, Moore JA, Hines JM: Regional changes in myocyte size and number in propranolol-treated hyperthyroid rats. Lab Invest 57: 708–713, 1987

    PubMed  CAS  Google Scholar 

  48. Zimmer H-G, Ibel H, Gerlach E: Significance of the hexose monophosphate shunt in experimentally induced cardiac hypertrophy. Basic Res Cardiol 75: 207–213, 1980

    Article  PubMed  CAS  Google Scholar 

  49. Zimmer H-G, Peffer H: Metabolic aspects of the development of experimental cardiac hypertrophy. Basic Res Cardiol 81 (Suppl 1): 127–137, 1986

    PubMed  CAS  Google Scholar 

  50. Heckmann M, Zimmer H-G: Effects of triiodothyronine in spontaneously hypertensive rats. Studies on cardiac metabolism, function and heart weight. Basic Res Cardiol 87: 333–343, 1992

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Physiology, University of Munich, Germany

    Heinz-Gerd Zimmer, Michael Irlbeck & Claudia Kolbeck-Rühmkorff

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  1. Heinz-Gerd Zimmer
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  2. Michael Irlbeck
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  3. Claudia Kolbeck-Rühmkorff
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Editors and Affiliations

  1. Institute for Heart Research, Slovak Academy of Sciences, Bratislava, Slovak Republic

    Ján Slezák  & Attila Ziegelhöffer  & 

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© 1995 Springer Science+Business Media Dordrecht

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Zimmer, HG., Irlbeck, M., Kolbeck-Rühmkorff, C. (1995). Response of the rat heart to catecholamines and thyroid hormones. In: Slezák, J., Ziegelhöffer, A. (eds) Cellular Interactions in Cardiac Pathophysiology. Developments in Molecular and Cellular Biochemistry, vol 14. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2005-4_14

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  • DOI: https://doi.org/10.1007/978-1-4615-2005-4_14

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-5828-2

  • Online ISBN: 978-1-4615-2005-4

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