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Measurement of left ventricular hemodynamic parameters in closed-chest rats under control and various pathophysiologic conditions

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Summary

A prototype of a recently developed mikro-tip® pressure transducer catheter (3 French) was inserted into the left ventricle of closed-chest rats via the right carotid artery. In thiobutabarbital-sodium anesthesia, heart rate (409±7 beats/min), left ventricular systolic pressure (LVSP: 142±4 mm Hg) and the maximal rate of rise of left ventricular pressure (LV dP/dtmax: 6073±187 mm Hg/sec, mean values ±SEM, n=19) were measured. The baroreceptor reflex was intact under these experimental conditions. In closed-chest guinea pigs, all functional parameters determined were lower (heart rate: 271±11 beats/min; LVSP: 94±6 mm Hg; LV dP/dtmax: 3248±295 mm Hg/sec, mean values ±SEM, n=9). To test the applicability of the new catheter tip manometer, cardiac hemodynamic parameters were determined in rats under various pathophysiologic conditions: Several periods of asphyxia were followed by progressive depressions in heart rate, LVSP and LV dP/dtmax, and acute as well as chronic stimulation with catecholamines (noradrenaline and isoproterenol) and with triiodothyronine was characterized by a pronounced positive inotropic and chronotropic effect. The technique described has many potential applications in cardiovascular studies on intact small laboratory animals.

Zusammenfassung

Der Prototyp eines neu entwickelten Katheter-Mikro-tip®-Manometers (3 French) wurde zur Katheterisierung des linken Ventrikels über die rechte A. carotis bei Ratten verwendet. In Thiobutabarbital-Narkose wurden Herzfrequenz (409±7 Schläge/min), linksventrikulärer systolischer Druck (LVSP: 142±4 mm Hg) und maximale Anstiegsgeschwindigkeit des linksventrikulären Druckes (LV dP/dtmax: 6073±187 mm Hg/s, Mittelwerte ±SEM, n=19) gemessen. Der Barorezeptoren-Reflex war unter diesen experimentellen Bedingungen intakt. Bei Meerschweinchen waren alle gemessenen Parameter der Herzfunktion niedriger (Herzfrequenz: 271±11 Schläge/min; LVSP: 94±6 mm Hg; LV dP/dtmax: 3248±295 mm Hg/s, Mittelwerte ±SEM, n=9). Um die Einsatzmöglichkeiten für das neue Katheter-tip-Manometer zu prüfen, wurden hämodynamische Parameter bei Ratten unter verschiedenen pathophysiologischen Bedingungen erfaßt: Mehrere Asphyxieperioden induzierten eine progressive Abnahme von Herzfrequenz, LVSP und LV dP/dtmax. Akute und chronische Stimulation mit Katecholaminen (Noradrenalin und Isoproterenol) und mit Trijodthyronin war durch einen ausgeprägten positiv inotropen und chronotropen Effekt gekennzeichnet. Die potentiellen Anwendungsmöglichkeiten der Methode für kardiovaskuläre Studien an intakten Kleintieren werden diskutiert.

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  1. H. -G. Zimmer

    Present address: Physiologisches Institut, Universität München, Pettenkoferstr. 12, D-8000, München 2

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  1. Physiological Institute of the University, Munich, Germany

    H. -G. Zimmer

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Zimmer, H.G. Measurement of left ventricular hemodynamic parameters in closed-chest rats under control and various pathophysiologic conditions. Basic Res Cardiol 78, 77–84 (1983). https://doi.org/10.1007/BF01923195

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