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Characterization of Epigenetic Biomarkers Using New Molecular Approaches

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Genomics-Assisted Crop Improvement

Abstract

Plants exhibit a polymorphism of DNA methylation status in their genomes in relation to various breeding traits and phenotypes. Evidence for relationships between DNA methylation and given phenotypes can be shown through the variations of phenotypes after treatments that alter DNA methylation percentages or through the variations of methylation percentages in different phenotypes. The corresponding “epialleles” are potential biomarkers for plant breeding selection. The target genes of these epigenetic modifications could be identified with a genome scanning approach using methyl-sensitive enzymes or methyl-binding affinity columns. Correlations between DNA methylation polymorphism and phenotypes could be tested using various methods such as bisulfite sequencing, physiological and genetic analyses. Identification of methylation biomarkers by these new molecular approaches have been successfully applied to human cancer detection and should be now envisaged for plant breeding selection.

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Authors and Affiliations

  1. Laboratoire de Biologie des Ligneux et des Grandes Cultures, UPRES EA 1207, rue de Chartres, BP 6759

    Marie-Véronique Gentil & Stéphane Maury

  2. Faculté des sciences, Université d’Orléans, 45067 Orléans Cedex 2, France

    Marie-Véronique Gentil

Authors
  1. Marie-Véronique Gentil
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  2. Stéphane Maury
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Editors and Affiliations

  1. ICRISAT, Patancheru, India

    Rajeev K. Varshney

  2. University of Bologna, Italy

    Roberto Tuberosa

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Gentil, MV., Maury, S. (2007). Characterization of Epigenetic Biomarkers Using New Molecular Approaches. In: Varshney, R.K., Tuberosa, R. (eds) Genomics-Assisted Crop Improvement. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6295-7_16

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