Abstract
Restriction Landmark Genomic Scanning (RLGS) profiles, which are based on the concept of using restriction enzyme sites as landmarks and are generated by two-dimensional gel electrophoresis, were applied to the analysis of plant genomes. This study identified landmark enzymes in RLGS profiles of rice, tobacco, and arabidopsis, because the success of RLGS analysis depends on finding useful landmarks and these are the most frequently studied higher plants. As the results demonstrate, in each plant RLGS analysis various landmark enzymes were identified as useful landmark enzymes. However, the number of spots in a single profile was smaller than in mouse RLGS profiles and differed remarkably in the various plants. In addition, we demonstrate the optimal electrophoresis conditions and a convenient spot-cloning method.
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Abbreviations
- EDTA:
-
disodium ethylenediamine tetraacetate
- rDNA:
-
ribosomal
- RNA:
-
gene
- TBE:
-
tris-borate
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Matsuyama, T., Abe, T., Bae, CH. et al. Adaptation of Restriction Landmark Genomic Scanning (RLGS) to plant genome analysis. Plant Mol Biol Rep 18, 331–338 (2000). https://doi.org/10.1007/BF02825060
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DOI: https://doi.org/10.1007/BF02825060