Abstract
The production of valuable compounds in industrial biotechnology is commonly done by cultivation of suspended cells or use of (immobilized) enzymes rather than using microorganisms in an immobilized state. Within the field of wastewater as well as odor treatment the application of immobilized cells is a proven technique. The cells are entrapped in a matrix of extracellular polymeric compounds produced by themselves. The surface-associated agglomerate of encapsulated cells is termed biofilm. In comparison to common immobilization techniques, toxic effects of compounds used for cell entrapment may be neglected. Although the economic impact of biofilm processes used for the production of valuable compounds is negligible, many prospective approaches were examined in the laboratory and on a pilot scale. This review gives an overview of biofilm reactors applied to the production of valuable compounds. Moreover, the characteristics of the utilized materials are discussed with respect to support of surface-attached microbial growth.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Warnock JN, Al-Rubeai M (2006) Bioreactor systems for the production of biopharmaceuticals from animal cells. Biotechnol Appl Biochem 45:1–12. doi:10.1042/ba2005023
Harding MW, Marques LLR, Howard RJ et al (2009) Can filamentous fungi form biofilms? Trends Microbiol 17(11):475–480. doi:10.1016/j.tim.2009.08.007
Fukuda H (1995) Immobilized microorganism bioreactors. In: Asenjo JA, Merchuk JC (eds) Bioreactor system design. Marcel Dekker Inc, New York, pp 339–375
Gross R, Schmid A, Buehler K (2012) Catalytic biofilms: a powerful concept for future bioprocesses. In: Lear G, Lewis GD (eds) Microbial biofilms. Caister Academic Press, Norfolk, pp 193–222
Kobayashi M, Shimizu S (2000) Nitrile hydrolases. Curr Opin Chem Biol 4(1):95–102. doi:10.1016/s1367-5931(99)00058-7
Murphy CD (2012) The microbial cell factory. Org Biomol Chem 10(10):1949–1957. doi:10.1039/c2ob06903b
Crueger W, Crueger A, Brock TD (1990) Biotechnology. A textbook of industrial microbiology, 2nd edn. Sinauer Associates, Sunderland
Kersters K, Lisdiyanti P, Komagata K et al (2006) The family Acetobacteracea: the genera Acetobacter, Acidomonas, Asaia, Gluconacetobacter, Gluconobacter, and Kozakia. In: Dworkin M (ed) Prokaryotes, vol 5. Springer Science + Business Media, New York, pp 163–200
Li XZ, Hauer B, Rosche B (2007) Single-species microbial biofilm screening for industrial applications. Appl Microbiol Biotechnol 76(6):1255–1262. doi:10.1007/s00253-007-1108-4
Cronenberg CCH, Ottengraf SPP, Vandenheuvel JC et al (1994) Influence of age and structure of penicillium chrysogenum pellets on the internal concentration profiles. Bioprocess Eng 10(5–6):209–216. doi:10.1007/bf00369531
Hooijmans CM, Briasco CA, Huang J et al (1990) Measurement of oxygen concentration gradients in gel-immobilized recombinant Escherichia coli. Appl Microbiol Biotechnol 33(6):611–618
Tijhuis L, van Loosdrecht MCM, Heijnen JJ (1994) Formation and growth of heterotrophic aerobic biofilms on small suspended particles in airlift reactors. Biotechnol Bioeng 44(5):595–608. doi:10.1002/bit.260440506
Demirci A, Pongtharangkul T, Pometto AL (2007) Application of biofilm reactors for production of value-added products by microbial fermentation. In: Blaschek HP, Wang HH, Agle ME (eds) Biofilms in the food environment. Blackwell Publishing Ltd., Oxford, pp 167–189
Gross R, Lang K, Buhler K et al (2010) Characterization of a biofilm membrane reactor and its prospects for fine chemical synthesis. Biotechnol Bioeng 105(4):705–717. doi:10.1002/bit.22584
Atkinson B, Black GM, Lewis PJS et al (1979) Biological particles of given size, shape, and density for use in biological reactors. Biotechnol Bioeng 21(2):193–200. doi:10.1002/bit.260210206
Karsakevich A, Ventina E, Vina I et al (1998) The effect of chemical treatment of stainless steel wire surfaces on Zymomonas mobilis cell attachment and product synthesis. Acta Biotechnol 18(3):255–265. doi:10.1002/abio.370180310
Schwartz T, Hoffmann S, Obst U (2003) Formation of natural biofilms during chlorine dioxide and u.v. disinfection in a public drinking water distribution system. J Appl Microbiol 95(3):591–601. doi:10.1046/j.1365-2672.2003.02019.x
Qureshi N, Paterson AHJ, Maddox IS (1988) Model for continuous production of solvents from whey permeate in a packed-bed reactor using cells of Clostridium acetobutylicum immobilized by adsorption onto bonechar. Appl Microbiol Biotechnol 29(4):323–328
Qureshi N, Annous BA, Ezeji TC et al (2005) Biofilm reactors for industrial bioconversion processes: employing potential of enhanced reaction rates. Microb Cell Fact 4:21. doi:10.1186/1475-2859-4-24
Jördening HJ (1992) Anaerobic biofilms in fluidized bed reactors. In: Melo LF, Bott TR, Fletcher M et al (eds) Biofilms—science and technology. Kluwer Academic Publishers, Dordrecht, pp 435–442
Sohling U, Ruf F, Neitmann E, Linke B (2010) Magnetische Glaspartikel zum Einsatz in Biogasanlagen, Fermentations- und Separationsprozessen(DE102010034083A1)
Lienhardt J, Schripsema J, Qureshi N et al (2002) Butanol production by Clostridium beijerinckii BA101 in an immobilized cell biofilm reactor—increase in sugar utilization. Appl Biochem Biotech 98:591–598. doi:10.1385/abab:98-100:1-9:591
Urek RO, Pazarlioglu NK (2004) A novel carrier for Phanerochaete chrysosporium immobilization. Artif Cell Blood Sub 32(4):563–574. doi:10.1081/labb-200039618
Asther M, Bellonfontaine MN, Capdevila C et al (1990) A thermodynamic model to predict Phanerochaete chrysosporium INA-12 adhesion to various solid carriers in relation to lignin peroxidase production. Biotechnol Bioeng 35(5):477–482. doi:10.1002/bit.260350505
Jones SC, Briedis DM (1992) Adhesion and lignin peroxidase production by the white-rot fungus Phanerochaete chrysosporium in a rotating biological contactor. J Biotechnol 24(3):277–290. doi:10.1016/0168-1656(92)90037-a
Guimarães C, Matos C, Azeredo J et al (2002) The importance of the morphology and hydrophobicity of different carriers on the immobilization and sugar refinery effluent degradation activity of Phanerochaete chrysosporium. Biotechnol Lett 24(10):795–800. doi:10.1023/a:1015580322450
Zhang SP, Norrlow O, Wawrzynczyk J et al (2004) Poly(3-hydroxybutyrate) biosynthesis in the biofilm of Alcaligenes eutrophus, using glucose enzymatically released from pulp fiber sludge. Appl Environ Microb 70(11):6776–6782. doi:10.1128/aem.70.11.6776- 6782.2004
Cotton JC, Pometto AL, Gvozdenovic-Jeremic J (2001) Continuous lactic acid fermentation using a plastic composite support biofilm reactor. Appl Microbiol Biotechnol 57(5–6): 626–630
Cheng KC, Demirci A, Catchmark JM (2010) Advances in biofilm reactors for production of value-added products. Appl Microbiol Biotechnol 87(2):445–456. doi:10.1007/s00253-010-2622-3
Park CH, Okos MR, Wankat PC (1989) Acetone-butanol-ethanol (ABE) fermentation in an immobilized cell trickle bed reactor. Biotechnol Bioeng 34(1):18–29. doi:10.1002/bit.260340104
Li XZ, Hauer B, Rosche B (2013) Catalytic biofilms on structured packing for the production of glycolic acid. J Microbiol Biotechnol 23(2):195–204. doi:10.4014/jmb.1207.07057
Gjaltema A, Vinke JL, van Loosdrecht MCM et al (1997) Abrasion of suspended biofilm pellets in airlift reactors: Importance of shape, structure, and particle concentrations. Biotechnol Bioeng 53(1):88–99. doi:10.1002/(sici)1097-0290(19970105)53:1<88:aid-bit12>3.0.co;2-5
Weusterbotz D, Aivasidis A, Wandrey C (1993) Continuous ethanol production by Zymomonas mobilis in a fluidized-bed reactor. Part II: Process development for the fermentation of hydrolysed B-starch without sterilization. Appl Microbiol Biotechnol 39(6):685–690
Barros AR, de Amorim ELC, Reis CM et al (2010) Biohydrogen production in anaerobic fluidized bed reactors: effect of support material and hydraulic retention time. Int J Hydrogen Energy 35(8):3379–3388. doi:10.1016/j.ijhydene.2010.01.108
Webb C, Fukuda H, Atkinson B (1986) The production of cellulase in a spouted bed fermenter using cells immobilized in biomass support particles. Biotechnol Bioeng 28(1):41–50. doi:10.1002/bit.260280107
Converti A, de Faveri D, Perego P et al (2006) Investigation on the transient conditions of a rotating biological contactor for bioethanol production. Chem Biochem Eng Q 20(4):401–406
Cao NJ, Du JX, Chen CS et al (1997) Production of fumaric acid by immobilized Rhizopus using rotary biofilm contactor. Appl Biochem Biotech 63–5:387–394. doi:10.1007/bf02920440
Delborghi M, Converti A, Parisi F et al (1985) Continuous alcohol fermentation in an immobilized cell rotating-disk reactor. Biotechnol Bioeng 27(6):761–768. doi:10.1002/bit.260270602
Sarkar S, Saha M, Roy D et al (2008) Enhanced production of antimicrobial compounds by three salt-tolerant actinobacterial strains isolated from the Sundarbans in a niche-mimic bioreactor. Mar Biotechnol 10(5):518–526. doi:10.1007/s10126-008-9090-0
Lewandowski Z, Beyenal H (2007) Fundamentals of biofilm research. CRC Press Inc., Boca Raton
Syron E, Casey E (2008) Membrane-aerated biofilms for high rate biotreatment: performance appraisal, engineering principles, scale-up, and development requirements. Environ Sci Technol 42(6):1833–1844. doi:10.1021/es0719428
Gross R, Hauer B, Otto K et al (2007) Microbial biofilms: New catalysts for maximizing productivity of long-term biotransformations. Biotechnol Bioeng 98(6):1123–1134. doi:10.1002/bit.21547
Halan B, Schmid A, Buchler K (2010) Maximizing the productivity of catalytic biofilms on solid supports in membrane aerated reactors. Biotechnol Bioeng 106(4):516–527. doi:10.1002/bit.22732
Gross R, Buehler K, Schmid A (2013) Engineered catalytic biofilms for continuous large scale production of n-octanol and (S)-styrene oxide. Biotechnol Bioeng 110(2):424–436. doi:10.1002/bit.24629
Barclay WR, Meager KM, Abril JR (1994) Heterotrophic production of long-chain omega-3-fatty-acids utilizing algae and algae-like microorganisms. J Appl Phycol 6(2):123–129. doi:10.1007/bf02186066
Kuhne S, Lakatos M, Foltz S et al (2013) Characterization of terrestrial cyanobacteria to increase process efficiency in low energy consuming production processes. Sustain Chem Proc 1(1):6. doi:10.1186/2043-7129-1-6
Mata TM, Martins AA, Caetano NS (2010) Microalgae for biodiesel production and other applications: a review. Renew Sustain Energy Rev 14(1):217–232
Boyd MR, Gustafson KR, McMahon JB et al (1997) Discovery of cyanovirin-N, a novel human immunodeficiency virus-inactivating protein that binds viral surface envelope glycoprotein gp120: potential applications to microbicide development. Antimicrob Agents Chemother 41(7):1521–1530
Bewley CA, Gustafson KR, Boyd MR et al (1998) Solution structure of cyanovirin-N, a potent HIV-inactivating protein. Nat Struct Biol 5(7):571–578
Bokesch HR, O’Keefe BR, McKee TC et al (2003) A potent novel anti-HIV protein from the cultured cyanobacterium Scytonema varium. Biochemistry 42(9):2578–2584
Sivonen K, Börner T (2008) Bioactive compounds produced by cyanobacteria. In: Herrero A, Flores EGF (eds) The cyanobacteria: molecular biology, genomics, and evolution. Caister Academic Press, Norfolk, pp 159–197
Belnap J, Lange OL (2001) Biological soil crusts: structure, function, and management. Ecological studies. Springer, Berlin
Lakatos M, Bilger W, Büdel B (2001) Carotenoid composition of terrestrial cyanobacteria: response to natural light conditions in habitats in Venezuela. Eur J Phycol 36:367–375
Dojani S, Lakatos M, Rascher U et al (2007) Nitrogen input by cyanobacterial biofilms of an inselberg into a tropical rainforest in French Guiana. Flora 202(7):521–529
Rascher U, Lakatos M, Büdel B et al (2003) Photosynthetic field capacity of cyanobacteria of a tropical inselberg of the Guiana Highlands. Eur J Phycol 38(3):247–256
Lange O, Bilger W, Rimke S et al (1989) Chlorophyll fluorescence of lichens containing green and blue green algae during hydration by water vapor uptake and by addition of liquid water. Bot Acta 102:306–313
Helm RF, Huang Z, Edwards D et al (2000) Structural characterization of the released polysaccharide of desiccation-tolerant Nostoc commune DRH-1. J Bacteriol 182(4):974–982
Shaw E, Hill DR, Brittain N et al (2003) Unusual water flux in the extracellular polysaccharide of the cyanobacterium Nostoc commune. Appl Environ Microb 69(9):5679–5684
Potts M (1999) Mechanisms of desiccation tolerance in cyanobacteria. Eur J Phycol 34(4):319–328
Fleming ED, Castenholz RW (2007) Effects of periodic desiccation on the synthesis of the UV-screening compound, scytonemin, in cyanobacteria. Environ Microbiol 9(6):1448–1455
Pereira S, Zille A, Micheletti E et al (2009) Complexity of cyanobacterial exopolysaccharides: composition, structures, inducing factors and putative genes involved in their biosynthesis and assembly. FEMS Microbiol Rev 33(5):917–941
de Marsac NT (1977) Occurrence and nature of chromatic adaptation in cyanobacteria. J Bacteriol 130(1):82–91
Kehoe DM, Gutu A (2006) Responding to color: the regulation of complementary chromatic adaptation. Annu Rev Plant Biol 57:127–150
Malcata FX (2011) Microalgae and biofuels: a promising partnership? Trends Biotechnol 29(11):542–549
Norsker N, Barbosa MJ, Vermuë MH et al (2011) Microalgal production—a close look at the economics. Biotechnol Adv 29(1):24–27
Krug TA, Daugulis AJ (1983) Ethanol-production using Zymomonas mobilis immobilized on an ion-exchange resin. Biotechnol Lett 5(3):159–164. doi:10.1007/bf00131895
Kunduru MR, Pometto AL (1996) Continuous ethanol production by Zymomonas mobilis and Saccharomyces cerevisiae in biofilm reactors. J Ind Microbiol 16(4):249–256. doi:10.1007/bf01570029
Kunduru MR, Pometto AL (1996) Evaluation of plastic composite-supports for enhanced ethanol production in biofilm reactors. J Ind Microbiol 16(4):241–248. doi:10.1007/bf01570028
Wang JL (2000) Production of citric acid by immobilized Aspergillus niger using a rotating biological contactor (RBC). Bioresour Technol 75(3):245–247
Urbance SE, Pometto AL, DiSpirito AA et al. (2003) Medium evaluation and plastic composite support ingredient selection for biofilm formation and succinic acid production by Actinobacillus succinogenes. Food Biotechnol 17(1). doi: 10.1081/fbt-120019984
Urbance SE, Pometto AL, DiSpirito AA et al. (2004) Evaluation of succinic acid continuous and repeat-batch biofilm fermentation by Actinobacillus succinogenes using plastic composite support bioreactors. Appl Microbiol Biotechnol 65(6). doi: 10.1007/s00253-004-1634-2
Lewis VP, Yang ST (1992) Continuous propionic-acid fermentation by immobilized Propionibacterium acidipropionici in a novel packed-bed bioreactor. Biotechnol Bioeng 40(4):465–474. doi:10.1002/bit.260400404
U.S. Department of Energy (2004) Top value added chemicals from biomass. Results of screening for potential candidates from sugars and synthetic gas, vol 1, Washington
Khiyami MA, Pometto AL, Kennedy WJ (2006) Ligninolytic enzyme production by Phanerochaete chrysosporium in plastic composite support biofilm stirred tank bioreactors. J Agr Food Chem 54(5). doi: 10.1021/jf051424l
Linko S (1988) Production and characterization of extracellular lignin peroxidase from immobilized Phanerochaete chrysosporium in a 10-l bioreactor. Enzyme Microb Technol 10(7):410–417. doi:10.1016/0141-0229(88)90035-X
Cho HY, Yousef AE, Yang ST (1996) Continuous production of pediocin by immobilized Pediococcus acidilactici P02 in a packed-bed bioreactor. Appl Microbiol Biotechnol 45(5):589–594
Hoover DG, Walsh PM, Kolaetis KM et al. (1988) A bacteriocin produced by Pediococcus species associated with a 5.5-megadalton plasmid. J Food Prot 51(1):29–31
Lozano JCN, Meyer JN, Sletten K et al (1992) Purification and amino acid sequence of bacteriocin produced by Pediococcus acidilactici. J Gen Microbiol 138:1985–1990
Liao CC, Yousef AE, Richter ER et al (1993) Pediococcus acidilactici PO2 bacteriocin production in whey permeate and inhibition of Listeria monocytogenes in foods. J Food Sci 58(2):430–434. doi:10.1111/j.1365-2621.1993.tb04291.x
Xia L, Chung YK, Yang ST et al (2005) Continuous nisin production in laboratory media and whey permeate by immobilized Lactococcus lactis. Process Biochem 40(1):13–24. doi:10.1016/j.procbio.2003.11.032
Pongtharangkul T, Demirci A (2006) Evaluation of culture medium for nisin production in a repeated-batch biofilm reactor. Biotechnol Progr 22(1). doi: 10.1021/bp050295q
Schügerl K (2005) Process development in biotechnology—a re-evaluation. Eng Life Sci 5(1):15–28. doi:10.1002/elsc.200402166
Srivastava P, Kundu S (1999) Studies on cephalosporin-C production in an air lift reactor using different growth modes of Cephalosporium acremonium. Process Biochem 34(4):329–333. doi:10.1016/s0032-9592(98)00059-4
Zhang CA, Zhu X, Liao QA et al (2010) Performance of a groove-type photobioreactor for hydrogen production by immobilized photosynthetic bacteria. Int J Hydrogen Energy 35(11):5284–5292. doi:10.1016/j.ijhydene.2010.03.085
Zhang HS, Bruns MA, Logan BE (2006) Biological hydrogen production by Clostridium acetobutylicum in an unsaturated flow reactor. Water Res 40(4):728–734. doi:10.1016/j.watres.2005.11.041
van Groenestijn JW, Geelhoed JS, Goorissen HP et al (2009) Performance and population analysis of a non-sterile trickle bed reactor inoculated with Caldicellulosiruptor saccharolyticus, a thermophilic hydrogen producer. Biotechnol Bioeng 102(5):1361–1367. doi:10.1002/bit.22185
Choudhary S, Schmidt-Dannert C (2010) Applications of quorum sensing in biotechnology. Appl Microbiol Biotechnol 86(5):1267–1279. doi:10.1007/s00253-010-2521-7
Demirci A, Pometto AL, Ho, K. L. G. (1997) Ethanol production by Saccharomyces cerevisiae in biofilm reactors. J Ind Microbiol Biotechnol 19(4). doi: 10.1038/sj.jim.2900464
Napoli F, Olivieri G, Russo ME et al (2010) Butanol production by Clostridium acetobutylicum in a continuous packed bed reactor. J Ind Microbiol Biotechnol 37(6):603–608. doi:10.1007/s10295-010-0707-8
Qureshi N, Maddox IS (1987) Continuous solvent production from whey permeate using cells of Clostridium acetobutylicum immobilized by adsorption onto bonechar. Enzyme Microb Technol 9(11):668–671. doi:10.1016/0141-0229(87)90125-6
Demirci A, Pometto AL, Johnson KE (1993) Evaluation of biofilm reactor solid support for mixed-culture lactic-acid production. Appl Microbiol Biotechnol 38(6):728–733
Demirci A, Pometto AL, Johnson KE (1993) Lactic-acid production in a mixed-culture biofilm reactor. Appl Environ Microbiol 59(1):203–207
Demirci A, Pometto AL (1995) Repeated-batch fermentation in biofilm reactors with plastic-composite supports for lactic-acid production. Appl Microbiol Biotechnol 43(4):585–589
Hekmat D, Bauer R, Fricke J (2003) Optimization of the microbial synthesis of dihydroxyacetone from glycerol with Gluconobacter oxydans. Bioprocess Biosyst Eng 26(2):109–116. doi:10.1007/s00449-003-0338-9
Casali S, Gungormusler M, Bertin L et al (2012) Development of a biofilm technology for the production of 1,3-propanediol (1,3-PDO) from crude glycerol. Biochem Eng J 64:84–90. doi:10.1016/j.bej.2011.11.012
Li XZ, Webb JS, Kjelleberg S et al (2006) Enhanced benzaldehyde tolerance in Zymomonas mobilis biofilms and the potential of biofilm applications in fine-chemical production. Appl Environ Microbiol 72(2):1639–1644. doi:10.1128/a-em.72.2.1639-1644.2006
Lee Y, Lee C, Chang H (1989) Citric acid production by Aspergillus niger immobilized on polyurethane foam. Appl Microbiol Biotechnol 30(2):141–143. doi:10.1007/BF00264001
Cheng K, Demirci A, Catchmark JM (2011) Continuous pullulan fermentation in a biofilm reactor. Appl Microbiol Biotechnol 90(3):921–927. doi:10.1007/s00253-011-3151-4
Cheng K, Catchmark JM, Demirci A (2009) Enhanced production of bacterial cellulose by using a biofilm reactor and its material property analysis. J Biol Eng 3:12. doi:10.1186/1754-1611-3-12
Khiyami MA, Al-Fadual SM, Bahklia AH (2011) Polyhydroxyalkanoates production via Bacillus plastic composite support (PCS) biofilm and date palm syrup. J Med Plants Res 5(14)
Pongtharangkul T, Demirci A (2006) Effects of fed-batch fermentation and pH profiles on nisin production in suspended-cell and biofilm reactors. Appl Microbiol Biotechnol 73(1):73–79. doi:10.1007/s00253-006-0459-6
Zhang HS, Bruns MA, Logan BE (2006) Biological hydrogen production by Clostridium acetobutylicum in an unsaturated flow reactor. Water Res 40(4):728–734. doi:10.1016/j.watres.2005.11.041
Govender S, Pillay VL, Odhav B (2010) Nutrient manipulation as a basis for enzyme production in a gradostat bioreactor. Enzyme Microbiol Technol 46(7):603–609. doi:10.1016/j.enzmictec.2010.03.007
Tsoligkas AN, Winn M, Bowen J et al (2011) Engineering Biofilms for Biocatalysis. ChemBioChem 12(9):1391–1395. doi:10.1002/cbic.201100200
Meleigy SA, Khalaf MA (2009) Biosynthesis of gibberellic acid from milk permeate in repeated batch operation by a mutant Fusarium moniliforme cells immobilized on loofa sponge. Bioresour Technol 100(1):374–379. doi:10.1016/j.biortech.2008.06.024
Acknowledgments
We wish to thank the German Research Foundation (DFG) for funding under LAÂ 1426/9-1, ULÂ 170/7-1, and SFBÂ 926/1-2013.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Muffler, K., Lakatos, M., Schlegel, C., Strieth, D., Kuhne, S., Ulber, R. (2014). Application of Biofilm Bioreactors in White Biotechnology. In: Muffler, K., Ulber, R. (eds) Productive Biofilms. Advances in Biochemical Engineering/Biotechnology, vol 146. Springer, Cham. https://doi.org/10.1007/10_2013_267
Download citation
DOI: https://doi.org/10.1007/10_2013_267
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-09694-0
Online ISBN: 978-3-319-09695-7
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)