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Potential for Osteogenic and Chondrogenic Differentiation of MSC

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Mesenchymal Stem Cells - Basics and Clinical Application I

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 129))

Abstract

The introduction of mesenchymal stem cells (MSC) into the field of tissue engineering for bone and cartilage repair is a promising development, since these cells can be expanded ex vivo to clinically relevant numbers and, after expansion, retain their ability to differentiate into different cell lineages. Mesenchymal stem cells isolated from various tissues have been intensively studied and characterized by many research groups. To obtain functionally active differentiated tissue, tissue engineered constructs are cultivated in vitro statically or dynamically in bioreactors under controlled conditions. These conditions include special cell culture media, addition of signalling molecules, various physical and chemical factors and the application of different mechanical stimuli. Oxygen concentration in the culture environment is also a significant factor which influences MSC proliferation, stemness and differentiation capacity. Knowledge of the different aspects which affect MSC differentiation in vivo and in vitro will help researchers to achieve directed cell fate without the addition of differentiation agents in concentrations above the physiological range.

Graphical Abstract

Antonina Lavrentieva and Tim Hatlapatka contributed equally.

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Abbreviations

ALP:

Alkaline phosphatase

AD:

Adipose-derived

b-FGF:

Basic fibroblast growth factor

BM:

Bone marrow

BMPs:

Bone morphogenic proteins

ESC:

Embryonic stem cell

GAG:

Glycosaminoglycan

GvHD:

Graft-versus-host disease

h:

Human

HA:

Hyaluronic acid

HLA:

Human leukocyte antigen

HUCPVC:

Human umbilical cord perivascular cells

ISCT:

International Society for Cellular Therapy

LIPUS:

Low-intensity pulsed ultrasound

LLLI:

Low-level light irradiation

MAPK:

Mitogen-activated protein kinases

MMPs:

Matrix metalloproteases

MSC:

Mesenchymal stromal cell

PBL:

Peripheral blood lymphocytes

PG:

Proteoglycan

PLGA:

Poly(L-lactide-co-glycolide)

PFF:

Pulsating fluid flow

r:

Rabbit

TGF-β:

Transforming growth factor-beta

TE:

Tissue engineering

UC:

Umbilical cord

UCB:

Umbilical cord blood

WJ:

Wharton’s jelly

3D:

Three-dimensional

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Authors and Affiliations

  1. Institut für Technische Chemie, Leibniz Universität Hannover, Callinstrasse 5, 30167, Hannover, Germany

    Antonina Lavrentieva

  2. Department für Biotechnologie, Universität für Bodenkultur, Muthgasse 18, 1190, Wien, Austria

    Tim Hatlapatka, Anne Neumann & Cornelia Kasper

  3. Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, Carl-Neuberg Strasse 1, 30625, Hannover, Germany

    Birgit Weyand

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  1. Antonina Lavrentieva
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  2. Tim Hatlapatka
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  3. Anne Neumann
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  4. Birgit Weyand
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  5. Cornelia Kasper
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Correspondence to Antonina Lavrentieva .

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Editors and Affiliations

  1. Abt. für Plastische und Handchirurgie, Medizinische Hochschule Hannover, Carl-Neubergstr. 1, Hannover, 30625, Germany

    Birgit Weyand

  2. Integrated Department of Oncology, Haematology and Respiratory Diseases, University of Modena, Modena, Italy

    Massimo Dominici

  3. Klinik für Frauenheilkunde und Geburtshi, Medizinische Hochschule Hannover, Carl-Neuberg-Str. 1, Hannover, 30625, Germany

    Ralf Hass

  4. Klinik für Immunologie und Rheumatologie, Medizinische Hochschule Hannover, Carl-Neuberg-Str. 1, Hannover, 30625, Niedersachsen, Germany

    Roland Jacobs

  5. Department für Biotechnologie, Universität für Bodenkultur, Muthgasse 18, Wien, 1190, Austria

    Cornelia Kasper

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Lavrentieva, A., Hatlapatka, T., Neumann, A., Weyand, B., Kasper, C. (2012). Potential for Osteogenic and Chondrogenic Differentiation of MSC. In: Weyand, B., Dominici, M., Hass, R., Jacobs, R., Kasper, C. (eds) Mesenchymal Stem Cells - Basics and Clinical Application I. Advances in Biochemical Engineering/Biotechnology, vol 129. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2012_133

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