Abstract
The free radical scavenging activity of various enzymatic extracts prepared from Hericium erinaceum was evaluated by using an ESR spectrometer. For this study, H. erinaceum was enzymatically hydrolyzed by eight carbohydrases and eight proteases. All of the hydrolyzed extracts scavenged DPPH, hydroxyl, superoxide, and alkyl radicals. Especially, the the pepsintreated extracts (PTE) exhibited the highest scavenging activity on hydroxyl radical, which is deemed of the strongest radical. Also, the neuroprotective effects of PTE against hydrogen peroxide-induced oxidative stress in PC12 cells were investigated. PTE stimulated the expression of Bcl-2, on the contrary, the extracts suppressed the expression of Bax. These results indicate that PTE possess antioxidative activity on PC12 cells.
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References
Chang R (1996) Functional properties of edible mushrooms. Nutr Rev 54, S 91–93.
Cheng HY, Lin TC, Yu KH, Yang CM, and Lin CC (2003) Antioxidant and free radical scavenging activities of Terminalia chebula. Biol Pharm Bull 26, 1331–1335.
Choi WS, Kim CJ, Park BS, Lee SE, Takeoka GR, Kim DG, Lanpiao X, and Kim JH (2005) Inhibitory effect on proliferation of vascular smooth muscle cells and protective effect on CCl(4)-induced hepatic damage of HEAI extracts. J Ethnopharmacol 100, 176–179.
Compori M (1985) Lipid peroxidation and cellular damage in toxic liver injury. Lab Invest 53, 599–620.
Cumby N, Zhong Y, Naczk M, and Shahidi F (2008) Antioxidant activity and water-holding capacity of canola protein hydrolysates. Food Chem 109, 144–148.
Fischer LR and Glass JD (2010) Oxidative stress induced by loss of Cu,Zn-superoxide dismutase (SOD1) or superoxide-generating herbicides causes axonal degeneration in mouse DRG cultures. Acta Neuropathol 119, 249–259.
Halliwell B (1997) Antioxidants and human disease: A general introduction. Nutr Rev 55, S44-S52.
Halliwell B (1999) Antioxidant defence mechanisms: from the beginning to the end (of the beginning). Free Radic Res 31, 261–272.
Hiramoto K, Johkoh H, Sako KI, and Kikugawa K (1993) DNA breaking activity of the carbon-centered radical generated from 2, 2-azobis(2-amidinopropane)-hydrochloride (AAPH). Free Radic Res Commun 19, 323–332.
Jia LM, Liu L, Dong Q, and Fang JN (2004) Structural investigation of a novel rhamnoglucogalactan isolated from the fruiting bodies of the fungus Hericium erinaceus. Carbohydr Res 339, 2667–2671.
Kalonia H, Kumar P, Nehru B, and Kumar A (2009) Neuroprotective effect of MK-801 against intra-striatal quinolinic acid induced behavioral, oxidative stress and cellular alterations in rats. Indian J Exp Biol 47, 880–892.
Kenmoku H, Shimai T, Toyomasu T, Kato N, Sassa T, and Erinacine Q (2002) A new erinacine from Hericium erinaceum, and its biosynthetic route to erinacine C in the basidiomycete. Biosci Biotechnol Biochem 66, 571–575.
Lee EW, Shizuki K, Hosokawa S, Suzuki M, Suganuma H, Inakuma T, Li J, Ohnishi-Kameyama M, Nagata Y, Furukawa S, and Kawagishi H (2000) Two novel diterpenoids, erinacines H and I from the mycelia of Hericium erinaceum. Biosci Biotechnol Biochem 64, 2402–2405.
Lee MW, Park SC, Kim JH, Kim IK, Han KS, Kim KY, Lee WB, Jung YK, and Kim SS (2002) The involvement of oxidative stress in tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in HeLa cells. Cancer Lett 182, 75–82.
Lee YL, Yen MT, and Mau JL (2007) Antioxidant properties of various extracts from Hypsizigus marmoreus. Food Chem 104, 1–9.
Li K and He Y (1999) Chemical studies on polysaccharides from mycelium of Hericium erinaceum (Bull. Ex Fr.) Pers. Zhongguo Zhong Yao Za Zhi 24, 742–744.
Mizuno T, Wasa T, Ito H, Suzuki C, and Ukai N (1992) Antitumor-active polysaccharides isolated from the fruiting body of Hericium erinaceum, an edible and medicinal mushroom called yamabushitake or houtou. Biosci Biotechnol Biochem 56, 347–348.
Nanjo F, Goto K, Seto R, Suzuki M, Sakai M, and Hara Y (1996) Scavenging effects of tea catechins and their derivatives on 1, 1-diphenyl-2-picrylhydrazyl radical. Free Radical Bio Med 21, 890–895.
Park PJ, Heo SJ, Park EJ, Kim SK, Byun HG, and Jeon BT (2005) Reactive oxygen scavenging effect of enzymatic extracts from Sargassum thunbergii. J Agr Food Chem 53, 6666–6672.
Pastorino JG, Chen ST, Tafani M, Snyder JW, and Farber J (1998) The overexpression of Bax produces cell death upon induction of the mitochondrial permeability transition. J Biol Chem 273, 7770–7775.
Slater TF (1984) Free-radical mechanisms in tissue injury. Biochem J 222, 1–15.
Vermes I, Haanen C, Steffens Nakken H, and Reutelingsperger C (1995) A novel assay for apoptosis: Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labeled annexin V. J Immunol Methods 184, 39–51.
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Lee, SJ., Kim, EK., Hwang, JW. et al. Neuroprotective effect of Hericium erinaceum against oxidative stress on PC12 cells. J. Korean Soc. Appl. Biol. Chem. 53, 283–289 (2010). https://doi.org/10.3839/jksabc.2010.044
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DOI: https://doi.org/10.3839/jksabc.2010.044