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Assessment of ecological diversity of rhizobacterial communities in vermicompost and analysis of their potential to improve plant growth

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Abstract

Present study was designed to determine the microbial diversity from three distinctive sites (amended with vermicompost) of Gujarat, India. A set of 76 strains were screened from total of 438 strains that exhibit plant growthpromoting (PGP) and antagonistic potential isolated from sites PS1 (Mehsana district), BS2 (Dantiwada district) and VS3 (Gandhinagar district). Their diversity indices were studied for determining the species richness and evenness of screened isolates. Results revealed that site BS2 showed the most significant diversity indices in terms of Shannon (H′ 1.525) and Simpson (1/D 5.120) than other two samples. Principal component analysis showed that bacterial diversity (H′) was correlated with the soil characteristics. Chickpea and groundnut plants inoculated with MBCU1 and MBCU3 isolates showed an increase in the vegetative growth parameters that evaluate plant growth when compared to uninoculated controls. Strains MBCU1 and MBCU3 were identified as Pseudomonas stutzeri and Pseudomonas mosselii, respectively, according to sequence analysis of the 16S rRNA gene. These both isolates belong to site BS2 and they showed specific PGP traits suggesting that these isolates can promote plant growth by more than one mechanism with respect to their higher diversity index.

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Abbreviations

PCA:

principal component analysis

PGP:

plant growth-promoting

PGPR:

plant growth-promoting rhizobacteria

PIE:

probability of an interspecific encounter

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Authors and Affiliations

  1. Department of Microbiology and Biotechnology, University School of Sciences, Gujarat University, Ahmedabad, 380009, Gujarat, India

    Urja Pandya & Meenu Saraf

  2. Department of Botany and Microbiology, Gurukul Kangri University, Hardwar, 249404, Uttarakhand, India

    Dinesh K. Maheshwari

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  1. Urja Pandya
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  2. Dinesh K. Maheshwari
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  3. Meenu Saraf
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Correspondence to Meenu Saraf.

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Pandya, U., Maheshwari, D.K. & Saraf, M. Assessment of ecological diversity of rhizobacterial communities in vermicompost and analysis of their potential to improve plant growth. Biologia 69, 968–976 (2014). https://doi.org/10.2478/s11756-014-0406-4

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