Applied Biochemistry and Biotechnology

, Volume 84, Issue 1, pp 5–37

Fundamental factors affecting biomass enzymatic reactivity


  • Vincent S. Chang
    • Department of Chemical EngineeringTexas A&M University
    • Department of Chemical EngineeringTexas A&M University

DOI: 10.1385/ABAB:84-86:1-9:5

Cite this article as:
Chang, V.S. & Holtzapple, M.T. Appl Biochem Biotechnol (2000) 84: 5. doi:10.1385/ABAB:84-86:1-9:5


Poplar wood was treated with peracetic acid, KOH, and ball milling to produce 147 modellignocelluloses with a broad spectrum of lignin contents, acetyl contents, and crystallinity indices (CrIs), respectively. An empirical model was identified that describes the roles of these three properties in enzymatic hydrolysis. Lignin content and CrI have the greatest impact on biomass digestibility, whereas acetyl content has a minor impact. The digestibility of several lime-treated biomass samples agreed with the empirical model. Lime treatment removesallacetyl groups and a moderate amount of lignin and increases CrIslightly; lignin removal is the dominant benefit from lime treatment.

Index Entries

Lignocellulose enzymatic digestibility lignin acetyl groups crystallinity correlation pretreatment lime

Copyright information

© Humana Press Inc 2000