Effect of active fraction isolated from the leaf extract of Dregea volubilis [Linn.] Benth. on plasma glucose concentration and lipid profile in streptozotocin-induced diabetic rats
The objective of the present study was to evaluate the effect of active fraction from Dregea volubilis [Linn.] Benth leaves on serum glucose and lipid profile in normal and diabetic rats.
Materials and methods
Diabetes was induced by streptozotocin in wistar rats. Petroleum ether, ethyl acetate, and ethanol extracts of Dregea volubilis [Linn.] Benth leaves were administered orally at a dose of 200 mg/kg, p.o. Metformin was used as standard Anti-diabetic drug (50 mg/kg, p.o). The extract showing for higher Anti-diabetic activity was subjected to column chromatography that led to isolation of an active fraction, which was given trivial name Dv-1. Dv-1 (100 mg/kg, p.o.) was studied for its hypoglycemic and hypolipidemic potential.
Ethanol extract was found to lower the Fasting blood glucose (FBG) level significantly (p < 0.05) in diabetic rats. Dv-1 caused a significant (p < 0.05) reduction in FBG level. Additionally it also caused reduction in cholesterol, triglyceride levels and improvement in the HDL level in diabetic rats.
Reduction in the FBG, cholesterol, triglyceride levels and improvement in the HDL by Dv-1 indicates that Dv-1 has Anti-diabetic activity along with anti hyperlipidemic efficacy and provides a scientific rationale for the use as an Anti-diabetic agent.
- Aikinson, MA, Maclaren, NK (1994) The pathogenesis of insulin dependent diabetes. N Engl J Med 331: pp. 1428-1436 CrossRef
- De-Fronzo, RA, Bonadonna, RC, Ferrannini, E (1997) Pathogenesis of NIDDM. International Text book of Diabetes mellitus. Chichester, John Wiley, England, pp. 635-712
- Donath, MY, Ehses, JA (2006) Type I and type II diabetes: NOD the diabetes we thought it was. Proc Natl Acad Sci 103: pp. 12217-12218 CrossRef
- Grover, JK, Yadav, SP (2004) Pharmacological actions and potential uses of Momordica charantia: a review. J Ethnopharmacol 93: pp. 123-132 CrossRef
- Hansotia, T, Drucker, DJ (2005) GIP and GLP-1 as incretin hormones: lessons from single and double incretin receptor knockout mice. Regul Pept 128: pp. 125-134 CrossRef
- Heber, MF, Ferreira, SR, Velez, LM (2013) Prenatal hyperandrogenism and lipid profile during different age stages: an experimental study. Fertil Steril 99: pp. 551-557 CrossRef
- Henry, NG, Yuan-Li, Z, Antonio, H (2005) Regulation of plasma triglycerides in insulin resistance and diabetes. Arch Med Res 36: pp. 232-240 CrossRef
- Jain, AK, Mohan, G, Vairale, RS (2010) Folklore claims on some medicinal plants used by Bheel tribe. Ind J Tradit Knowl 9: pp. 105-107
- Jianfeng, C, Chaopin, L, Pengying, Z (2012) Anti diabetic effect of burdock (Arctium lappa L.) root ethanolic extract on streptozotocin-induced diabetic rats. African J Biotech 11: pp. 9079-9085
- Jing, P, Gong, P, Linghai, L (2011) Palmitic acid acutely stimulates glucose uptake via activation of Akt and ERK1/2 in skeletal muscle cells. J Lipid Res 52: pp. 1319-1327 CrossRef
- Kahn, CR, Shechter, Y Insulin oral hypoglycemic agents and the pharmacology of the endocrine pancreas in the pharmacological basis of therapeutics. In: Gillman, AG, Rail, TW, Nies, AS, Taylor, P eds. (1991) Insulin oral hypoglycemic agents and the pharmacology of the endocrine pancreas in the pharmacological basis of therapeutics. Pergamon press, New York, pp. 1463-1495
- Kavishankar, N, Lakshmidevi, S, Mahadeva Murthy, HS (2011) Diabetes and medicinal plants-a review. Int J Pharm Biomed Sci 2: pp. 65-80
- King, H, Aubert, RE, Herman, WH (1998) Global burden of diabetes 1995–2025: prevalence, numerical estimates, and projection. Diabetes Care 21: pp. 1414-1431 CrossRef
- Masana, L, Ibarretxe, D, Heras, M (2012) Substituting non-HDL cholesterol with LDL as a guide for lipid-lowering therapy increases the number of patients with indication for therapy. Atherosclerosis 226: pp. 471-475 CrossRef
- Pandikumar, P, Ayyanar, M, Ignacimuthu, S (2007) Medicinal plants used by Malasar tribes of Coimbatore district, Tamil Nadu. Indian J Trad Knowledge 6: pp. 579-582
- Pepato, MT, Mori, DM, Baviera, AM (2005) Fruit of the jambolan tree (Eugenia jambolana) and experimental diabetes. J Ethnopharmacol 96: pp. 43-48 CrossRef
- Rajadurai, M, Vidhya, VG, Ramya, M (2009) Ethno-medicinal plants used by the traditional healers of Pacchamalai hills, Tamil Nadu, India. J Ethnobiol Ethnomed 3: pp. 39-41
- Roman, LJ, Palmer, CNA, Clark, JE (1993) Expression of rabbit cytochromes P-450 which catalyze the ώ-hydroxylation of arachidonic acid, fatty acid and prostaglandins. Arch Biochem Biophys 307: pp. 57-65 CrossRef
- Saha, HR, Sarkar, BC, Khan, SA, Sana, NK, Choudhury, S (2012) A comparative study of thyroid hormone and lipid status in diabetic and non diabetic adults. Open Access Sci Reports 1: pp. 1-5
- Silija, VP, SamithaVarma, K, Mohanan, KV (2008) Ethnomedicinal plant knowledge of the Mullukuruma tribe of Wayanad district, Kerala. Indian J Trad Knowledge 7: pp. 612-614
- Sriplang, K, Adisakwattana, K, Rungsipipat, K, Yibchok-anun, K (2007) Effects of Orthosiphon stamineus aqueous extract on plasma glucose concentration and lipid profile in normal and streptozotocin-induced diabetic rats. J Ethnopharmacol 109: pp. 510-514 CrossRef
- Takeshi, K, Shoichi, N, Yasunori, K (2002) Report of the committee on the classification and diagnostic criteria of diabetes mellitus. Diabetes Res Clin Pract 55: pp. 65-85 CrossRef
- Tripathi, J, Kumari, R, Vrish Dhwaj, A (2012) Anti-diabetic activity of Diplocyclos palmatus Linn. in Streptozotocin-induced diabetic mice. Ind J Pharm Edu Res 46: pp. 352-359
- Effect of active fraction isolated from the leaf extract of Dregea volubilis [Linn.] Benth. on plasma glucose concentration and lipid profile in streptozotocin-induced diabetic rats
- Open Access
- Available under Open Access This content is freely available online to anyone, anywhere at any time.
- Online Date
- August 2013
- Online ISSN
- Springer International Publishing
- Additional Links
- Blood glucose
- Hexadecenoic acid
- Dregea volubilis [Linn.] Benth
- Lipid profile