Skip to main content
SpringerLink
Log in

The pathogenetic importance of C774T single nucleotide polymorphism of the endothelial nitric oxide synthase gene in the development of metabolic syndrome

  • Published:
Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry Aims and scope Submit manuscript

Abstract

The relationship between nitric oxide production and metabolic disorders and the role of endothelial nitric oxide synthase (eNOS or NOS3) in metabolic syndrome (MS) remain poorly understood and need deeper investigation. In this context the role of the NOS3 gene in pathogenesis of MS is of special interest. The aim of the study was to investigate association of NOS3 single nucleotide polymorphism C774T with risk of MS in the Slavic population of the Kaliningrad region and the relationship of this polymorphic variant with some parameters of endothelial dysfunction. The study included 128 patients (48 men and 80 women aged from 36 to 52 years) with MS. The control group consisted of 126 healthy volunteers (60 men and 66 women aged from 30 to 40 years). Genotyping was performed by real-time PCR. Serum nitrite levels were determined spectrophotometrically by the Griess method. Serum levels of endothelin-1 and eNOS were evaluated by ELISA. The study has shown association of T allele (OR = 2.06; p = 0.0004; CI: 1.38–3.08) and CT genotype (OR = 1.97; p = 0.014; CI: 1.14–3.40 ) C774T polymorphism of the NOS3 gene with risk of MS in the Slavic population of the Kaliningrad region. Allele C (OR = 0.48; p = 0.0004; CI: 0.32–0.72) and homozygous CC genotype (OR = 0.41; p = 0.001; CI: 0.24–0.69) C774T polymorphism of the NOS3 gene were associated with reduced risk of the development of MS. Significant differences in serum levels of eNOS and endothelin-1 depended on the CT and TT genotypes of C774T polymorphism of the NOS3 gene in MS.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Alberti, K.G., Zimmet, P., and Shaw, J., Diabet. Med., 2006, vol. 23, pp. 469–480.

    Article  CAS  Google Scholar 

  2. Bredt, D.S., Free Radic. Res., 1999, vol. 31, no. 6, pp. 577–596.

    Article  CAS  Google Scholar 

  3. Tanus-Santos, J.E., Desai, M., and Flockhart, D.A., Pharmacogenetics, 2001, vol. 11, no. 8, pp. 719–725.

    Article  CAS  Google Scholar 

  4. Moshage, H., Kok, B., Huizenga, J.R., and Jansen, P.L., Clin. Chem., 1995, vol. 41, no. 6, Pt 1, pp. 892–896.

    CAS  Google Scholar 

  5. Novoradovsky, A., Brantly, M.L., Waclawiw, M.A., Chaudhary, P.P., Ihara, H., Qi, L., Eissa, N.T., Barnes, P.M., Gabriele, K.M., Ehrmantraut, M.E., Rogliani, P., and Moss, J., Am. J. Respir. Cell. Mol. Biol., 1999, vol. 20, no. 3, pp. 441–447.

    Article  CAS  Google Scholar 

  6. Hunt, R., Sauna, Z.E., Ambudkar, S.V., Gottesman, M.M., and Kimchi-Sarfaty, C., Methods Mol. Biol., 2009, vol. 578, pp. 23–39.

    Article  CAS  Google Scholar 

  7. Holla, L.I., Jurajda, M., Pohunek, P., and Znojil, V., Hum. Immunol., 2008, vol. 69, nos. 4–5, pp. 306–313. doi 10.1016/jhumimm.2008.03.003

    Article  CAS  Google Scholar 

  8. Taverna, M.J., Elgrably, F., Selmi, H., Selam, J.L., and Slama, G., Nitric Oxide, 2005, vol. 13, no. 1, pp. 88–92.

    Article  CAS  Google Scholar 

  9. Kondrat’eva, E.I., Puzyrev, V.P., Tarasenko, N.V., Kosyankova, T.V., Milovanova, T.D., Gulieva, N.G., and Gudkova, T.K., Sakharnyi Diabet, 2007, vol. 2, pp. 9–13.

    Google Scholar 

  10. Ruef, J., Moser, M., Kübler, W., and Bode, C., Cardiovasc. Pathol., 2001, vol. 10, no. 6, pp. 311–315.

    Article  CAS  Google Scholar 

  11. Metzger, I.F., Sertório, J.T., and Tanus-Santos, J.E., Free Radic. Biol. Med., 2007, vol. 43, no. 6, pp. 987–992.

    Article  CAS  Google Scholar 

  12. Caimi, G., Lo Presti, R., Montana, M., Noto, D., Canino, B., Averna, M.R., and Hopps, E., Oxid. Med. Cell. Longev., 2014, vol. 2014, 824756. doi 10.1155/2014/824756

  13. Ueyama, J., Kondo, T., Imai, R., Kimata, A., Yamamoto, K., Suzuki, K., Inoue, T., Ito, Y., Miyamoto, K., Hasegawa, T., and Hamajima, N., Environ. Health Prev. Med., 2008, vol. 13, no. 1, pp. 36–42. doi 10.1007/s12199-007-0001-1

    Article  CAS  Google Scholar 

  14. Tessari, P., Cecchet, D., Cosma, A., Vettore, M., Coracina, A., Millioni, R., Iori, E., Puricelli, L., Avogaro, A., and Vedovato, M., Diabetes, 2010, vol. 59, no. 9, pp. 2152–2159. doi 10.2337/db09-1772

    Article  CAS  Google Scholar 

  15. Avogaro, A., Toffolo, G., Kiwanuka, E., de Kreutzenberg, S.V., Tessari, P., and Cobelli, C., Diabetes, 2003, vol. 52, no. 3, pp. 795–802.

    Article  CAS  Google Scholar 

  16. Weinberg, J.B., Molecular Medicine, 1998, vol. 4, no. 9, pp. 557–591.

    CAS  Google Scholar 

  17. Pacher, P., Beckman, J.S., Liaudet, L., Physiol. Rev., 2007, vol. 87, no. 1, pp. 315–424.

    Article  CAS  Google Scholar 

  18. Tsuchiya, K., Sakai, H., Suzuki, N., Iwashima, F., Yoshimoto, T., Shichiri, M., and Hirata, Y., Endocrinology, 2007, vol. 148, pp. 4548–4556.

    Article  CAS  Google Scholar 

  19. Noronha, B.T., Li, J.M., Wheatcroft, S.B., Shah, A.M., and Kearney, M.T., Diabetes, 2005, vol. 54, pp. 1082–1089.

    Article  CAS  Google Scholar 

  20. Hemmrich, K., Suschek, C.V., Lerzynski, G., and Kolb-Bachofen, V., J. Appl. Physiol., 2003, vol. 95, pp. 1937–1946. doi 10.1152/japplphysiol. 00419.2003

    Article  CAS  Google Scholar 

  21. Cosentino, F., Patton, S., d’Uscio, L.V., Werner, E.R., Werner-Felmayer, G., Moreau, P., Malinski, T., and Lüscher, T.F., J. Clin. Invest., 1998, vol. 101, pp. 1530–1537.

    Article  CAS  Google Scholar 

  22. Clapp, B.R., Hingorani, A.D., Kharbanda, R.K., Mohamed-Ali, V., Stephens, J.W., Vallance, P., and MacAllister, R.J., Cardiovasc. Res., 2004, vol. 64, no. 1, pp. 172–178.

    Article  CAS  Google Scholar 

  23. Dimmeler, S., Fleming, I., Fisslthaler, B., Hermann, C., Busse, R., and Zeiher, A.M., Nature, 1999, vol. 399, no. 6736, pp. 601–605.

    Article  CAS  Google Scholar 

  24. Buga, G.M., Griscavage, J.M., Rogers, N.E., and Ignarro, L.J., Circ. Res., 1993, vol. 73, pp. 808–812.

    Article  CAS  Google Scholar 

  25. Hink, U., Li, H., Mollnau, H., Oelze, M., Matheis, E., Hartmann, M., Skatchkov, M., Thaiss, F., Stahl, R.A., Warnholtz, A., Meinertz, T., Griendling, K., Harrison, D.G., Forstermann, U., and Munzel, T., Circ. Res., 2001, vol. 88, no. 2, E14–22.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Immanuel Kant Baltic Federal University, ul. Alexandra Nevskogo 14, Kaliningrad, 236041, Russia

    N. S. Fattakhov, M. A. Vasilenko, D. A. Skuratovskaia, D. I. Kulikov, E. V. Kirienkova & L. S. Litvinova

  2. Regional Clinical Hospital of the Kaliningrad Region, ul. Klinicheskaya 74, Kaliningrad, 236016, Russia

    P. A. Zatolokin

  3. Municipal Childrens Hospital No. 6, ul. Gorkogo 203-203a, Kaliningrad, 236029, Russia

    M. A. Beletskaya

Authors
  1. N. S. Fattakhov
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. A. Vasilenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. A. Skuratovskaia
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. D. I. Kulikov
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. E. V. Kirienkova
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. P. A. Zatolokin
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. M. A. Beletskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. L. S. Litvinova
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to L. S. Litvinova.

Additional information

Original Russian Text © N.S. Fattakhov, M.A. Vasilenko, D.A. Skuratovskaia, D.I. Kulikov, E.V. Kirienkova, P.A. Zatolokin, M.A. Beletskaya, L.S. Litvinova, 2016, published in Biomeditsinskaya Khimiya.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Fattakhov, N.S., Vasilenko, M.A., Skuratovskaia, D.A. et al. The pathogenetic importance of C774T single nucleotide polymorphism of the endothelial nitric oxide synthase gene in the development of metabolic syndrome. Biochem. Moscow Suppl. Ser. B 10, 81–86 (2016). https://doi.org/10.1134/S1990750816010054

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1990750816010054

Keywords

Search

Navigation