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Association of Glu298Asp Polymorphism of Endothelial NO Synthase Gene with Metabolic Syndrome Development: a Pilot Study

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We studied association of single nucleotide polymorphism Glu298Asp (rs1799983) of the NOS3 gene with the risk of metabolic syndrome in the Slavic population. Blood samples were obtained from 128 patients with metabolic syndrome and 100 healthy individuals. Polymorphism Glu298Asp of the NOS3 gene was genotyped by allele-specific PCR. Allele Asp (OR=1.95, 95%CI 1.29-2.95, p=0.007) and genotype Asp/Asp (OR=2.56, 95%CI 0.98-6.72, p=0.04) were associated with the risk of metabolic syndrome in Slavic population. Patients with metabolic syndrome carrying genotype Asp/Asp had higher serum endothelin-1 level in comparison with Glu/Asp and Glu/Glu carriers.

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References

  1. Albrecht EW, Stegeman CA, Heeringa P, Henning RH, van Goor H. Protective role of endothelial nitric oxide synthase. J. Pathol. 2003;199(1):8-17.

    Article  PubMed  Google Scholar 

  2. Alkharfy KM, Al-Daghri NM, Al-Attas OS, Alokail MS, Mohammed AK, Vinodson B, Clerici M, Kazmi U, Hussain T, Draz HM. Variants of endothelial nitric oxide synthase gene are associated with components of metabolic syndrome in an Arab population. Endocr. J. 2012;59(3):253-263.

    Article  CAS  PubMed  Google Scholar 

  3. DeSouza CA, Van Guilder GP, Greiner JJ, Smith DT, Hoetzer GL, Stauffer BL. Basal endothelial nitric oxide release is preserved in overweight and obese adults. Obes. Res. 2005;13(8):1303-1306.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Duplain H, Burcelin R, Sartori C, Cook S, Egli M, Lepori M, Vollenweider P, Pedrazzini T, Nicod P, Thorens B, Scherrer U. Insulin resistance, hyperlipidemia, and hypertension in mice lacking endothelial nitric oxide synthase. Circulation. 2001;104(3):342-345.

    Article  CAS  PubMed  Google Scholar 

  5. El-Baz RA, Wafa A, Hosny NM, El-Saka SR. Biochemical study on endothelial nitric oxide gene polymorphism in fatty liver patients. IOSR J. Pharm. Biol. Sci. (IOSR-JPBS). 2015;10(6, ver. 1):26-33. doi: 10.9790/3008-10612633.

    Google Scholar 

  6. Feron O, Saldana F, Michel JB, Michel T. The endothelial nitric-oxide synthase-caveolin regulatory cycle. J. Biol. Chem. 1998;273(6):3125-3128.

    Article  CAS  PubMed  Google Scholar 

  7. Hsieh MC, Hsiao JY, Tien KJ, Chang SJ, Lin PC, Hsu SC, Liang HT, Chen HC, Lin SR. The association of endothelial nitric oxide synthase G894T polymorphism with C-reactive protein level and metabolic syndrome in a Chinese study group. Metabolism. 2008;57(8):1125-1129.

    Article  CAS  PubMed  Google Scholar 

  8. Imamura A, Takahashi R, Murakami R, Kataoka H, Cheng XW, Numaguchi Y, Murohara T, Okumura K. The effects of endothelial nitric oxide synthase gene polymorphisms on endothelial function and metabolic risk factors in healthy subjects: the significance of plasma adiponectin levels. Eur. J. Endocrinol. 2008;158(2):189-195.

    Article  CAS  PubMed  Google Scholar 

  9. Lauer T, Preik M, Rassaf T, Strauer BE, Deussen A, Feelisch M, Kelm M. Plasma nitrite rather than nitrate reflects regional endothelial nitric oxide synthase activity but lacks intrinsic vasodilator action. Proc. Natl Acad. Sci. USA. 2001;98(22):12,814-12,819.

    Article  CAS  Google Scholar 

  10. Liu CS, Huang RJ, Sung FC, Lin CC, Yeh CC. Association between endothelial nitric oxide synthase polymorphisms and risk of metabolic syndrome. Dis. Markers. 2013;34(3):187-197.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Miranda JA, Belo VA, Souza-Costa DC, Lanna CM, Tanus-Santos JE. eNOS polymorphism associated with metabolic syndrome in children and adolescents. Mol. Cell. Biochem. 2013;372(1-2):155-160.

    Article  CAS  PubMed  Google Scholar 

  12. Piccoli JC, Gottlieb MG, Castro L, Bodanese LC, Manenti ER, Bogo MR, Peres A, Rocha MI, Cruz IB. Association between 894G>T endothelial nitric oxide synthase gene polymorphisms and metabolic syndrome. Arq. Bras. Endocrinol. Metabol. 2008;52(8):1367-1373.

    Article  PubMed  Google Scholar 

  13. Saini V, Bhatnagar MK, Bhattacharjee J. Association of endothelial dysfunction with endothelin, nitric oxide and eNOS Glu298Asp gene polymorphism in coronary artery disease. Dis. Markers. 2011;31(4):215-222.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Tesauro M, Thompson WC, Rogliani P, Qi L, Chaudhary PP, Moss J. Intracellular processing of endothelial nitric oxide synthase isoforms associated with differences in severity of cardiopulmonary diseases: cleavage of proteins with aspartate vs. glutamate at position 298. Proc. Natl Acad. Sci. USA. 2000;97(6):2832-2835.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Tziomalos K, Athyros VG, Karagiannis A, Mikhailidis DP. Endothelial dysfunction in metabolic syndrome: prevalence, pathogenesis and management. Nutr. Metab. Cardiovasc. Dis. 2010;20(2):140-146.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Immanuel Kant Baltic Federal University, Kaliningrad, Russia

    N. S. Fattakhov, D. A. Skuratovskaya, M. A. Vasilenko, E. V. Kirienkova & L. S. Litvinova

  2. Regional Hospital of the Kaliningrad Region, Kaliningrad, Russia

    P. A. Zatolokin & N. I. Mironyuk

  3. Mental Health Research Institute, Tomsk National Research Medical Center of the Russian Academy of Sciences, Tomsk, Russia

    N. S. Fattakhov

Authors
  1. N. S. Fattakhov
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  2. D. A. Skuratovskaya
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  3. M. A. Vasilenko
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  4. E. V. Kirienkova
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  5. P. A. Zatolokin
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  6. N. I. Mironyuk
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  7. L. S. Litvinova
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Corresponding author

Correspondence to N. S. Fattakhov.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 162, No. 11, pp. 563-566, November, 2016

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Fattakhov, N.S., Skuratovskaya, D.A., Vasilenko, M.A. et al. Association of Glu298Asp Polymorphism of Endothelial NO Synthase Gene with Metabolic Syndrome Development: a Pilot Study. Bull Exp Biol Med 162, 615–618 (2017). https://doi.org/10.1007/s10517-017-3670-9

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  • DOI: https://doi.org/10.1007/s10517-017-3670-9

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