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The effect of plant hormones on the components of the secretory pathway in human normal and tumor cells

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Abstract

Plant hormones play a key role in plant growth and differentiation. Certain plant hormones are known to be potential antitumor agents, affect the secretory activity of animal cells, and are produced by mammalian cells as proinflammatory cytokines. The goal of this research was to study the effect of abscisic and gibberellic acids on the secretory system of human epidermoid A431 carcinoma cells and HaCaT keratinocytes. Immunocytochemical and morphometric analysis showed that a subtoxic concentration of abscisic and gibberellic acids induced extension of the ER network and increased the size of the Golgi complex. Electron-microscope studies confirmed the hypertrophic changes of the Golgi complex: swelling of cisternae in the trans-Golgi compartment after exposure to abscisic acid and swelling of cis- and trans-compartments after exposure to gibberellic acid. The Click-iT technique revealed elevation of total protein synthesis only in A431 cells exposed to abscisic acid. Our data suggest that the hypertrophy of Golgi may reflect enhanced secretory activity in A431 cells exposed to abscisic acid. In other experiments, Golgi hypertrophy was not accompanied with increased protein synthesis that suggested the stress-related changes of ER and Golgi complex. Our results demonstrate that morphologically similar reaction manifested in hypertrophy of Golgi complex, in response to plant hormones, is the result of different functional activities, and that molecular mechanisms underlying induced changes need further investigations.

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Abbreviations

ABA:

abscisic acid

GA:

gibberellic acid

DDT:

dithiothreitol

UV:

ultraviolet

ER:

endoplasmic reticulum

MTT:

3-(4,5-dymethyl-2-thiazolyl)-2,5-dyphenyl-2H-tetrazolium bromide

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Authors and Affiliations

  1. Moscow State University, Moscow, Russia

    M. S. Vildanova, M. A. Savitskaya, G. E. Onishchenko & E. A. Smirnova

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  1. M. S. Vildanova
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  2. M. A. Savitskaya
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  3. G. E. Onishchenko
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  4. E. A. Smirnova
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Correspondence to M. S. Vildanova.

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Original Russian Text © M.S. Vildanova, M.A. Savitskaya, G.E. Onishchenko, E.A. Smirnova, 2014, published in Tsitologiya, 2014, Vol. 56, No. 7, pp. 516–525.

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Vildanova, M.S., Savitskaya, M.A., Onishchenko, G.E. et al. The effect of plant hormones on the components of the secretory pathway in human normal and tumor cells. Cell Tiss. Biol. 8, 407–415 (2014). https://doi.org/10.1134/S1990519X14050095

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  • DOI: https://doi.org/10.1134/S1990519X14050095

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