Abstract
Anthocyanins are a class of natural water-soluble pigments found in plants, which are responsible for the varied colours of vegetative tissues and reproductive organs. The regulation of anthocyanin biosynthesis requires multiple factors, including enzymes, structural or regulatory genes, and transcriptional regulator proteins. While there has been notable advancement in comprehending anthocyanin biosynthesis, encompassing its structures and overarching regulation, there remains a gap in our understanding concerning the intricate interactions among various genes, molecular and biochemical mechanisms, and their feedback responses to anthocyanin regulatory networks. This article aims to provide an overview of the current understanding of the complex regulatory networks involved in the phenylpropanoid pathway, with a specific focus on anthocyanin biosynthesis.
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References
Aguilar-Barragán A, Ochoa-Alejo N (2014) Virus-induced silencing of MYB and WD40 transcription factor genes affects the accumulation of anthocyanins in chilli pepper fruit. Biol Plant 58(3):567–574
Albert NW, Lewis DH, Zhang H, Schwinn KE, Jameson PE, Davies KM (2011) Members of an R2R3-MYB transcription factor family in Petunia are developmentally and environmentally regulated to control complex floral and vegetative pigmentation patterning. Plant J 65(5):771–784
An JP, Yao JF, Xu RR, You CX, Wang XF, Hao YJ (2018) Apple bZIP transcription factor MdbZIP44 regulates abscisic acid-promoted anthocyanin accumulation. Plant Cell Environ 41(11):2678–2692
An JP, Zhang XW, You CX, Bi SQ, Wang XF, Hao YJ (2019) Md WRKY 40 promotes wounding-induced anthocyanin biosynthesis in association with Md MYB 1 and undergoes Md BT 2-mediated degradation. New Phytol 224(1):380–395
An JP, Wang XF, Zhang XW, Xu HF, Bi SQ, You CX, Hao YJ (2020) An apple MYB transcription factor regulates cold tolerance and anthocyanin accumulation and undergoes MIEL1-mediated degradation. Plant Biotechnol J 18(2):337–353
An JP, Wang XF, You CX, Hao YJ (2021) The anthocyanin biosynthetic regulator MdMYB1 positively regulates ascorbic acid biosynthesis in apple. Front Agric Sci Eng 8:231–235
Aza-Gonzalez C, Herrera-Isidrón L, Núñez-Palenius HG, Martínez De La Vega O, Ochoa-Alejo N (2013) Anthocyanin accumulation and expression analysis of biosynthesis-related genes during chili pepper fruit development. Biol Plant 57(1):49–55
Barbagallo RN, Palmeri R, Fabiano S, Rapisarda P, Spagna G (2007) Characteristic of β-glucosidase from Sicilian blood oranges in relation to anthocyanin degradation. Enzyme Microb Technol 41(5):570–575
Baudry A, Caboche M, Lepiniec L (2006) TT8 controls its own expression in a feedback regulation involving TTG1 and homologous MYB and bHLH factors, allowing a strong and cell-specific accumulation of flavonoids in Arabidopsis thaliana. Plant J 46(5):768–779
Belwal T, Singh G, Jeandet P, Pandey A, Giri L, Ramola S et al (2020) Anthocyanins, multi-functional natural products of industrial relevance: recent biotechnological advances. Biotechnol Adv 43:107600
Ben-Simhon Z, Judeinstein S, Nadler-Hassar T, Trainin T, Bar-Ya’akov I, Borochov-Neori H, Holland D (2011) A pomegranate (Punica granatum L.) WD40-repeat gene is a functional homologue of Arabidopsis TTG1 and is involved in the regulation of anthocyanin biosynthesis during pomegranate fruit development. Planta 234:865–881
Biała W, Jasiński M (2018) The phenylpropanoid case–it is transport that matters. Front Plant Sci 9:1610
Birkenbihl RP, Kracher B, Roccaro M, Somssich IE (2017) Induced genome-wide binding of three Arabidopsis WRKY transcription factors during early MAMP-triggered immunity. Plant Cell 29(1):20–38
Borah P, Khurana JP (2018) The OsFBK1 E3 ligase subunit affects anther and root secondary cell wall thickenings by mediating turnover of a cinnamoyl-CoA reductase. Plant Physiol 176(3):2148–2165
Carvalho LC, Vidigal P, Amâncio S (2015) Oxidative stress homeostasis in grapevine (Vitis vinifera L.). Front Environ Sci 3:20
Chacón-Cerdas R, Barboza-Barquero L, Albertazzi FJ, Rivera-Méndez W (2020) Transcription factors controlling biotic stress response in potato plants. Physiol Mol Plant Pathol 112:101527
Chapman JM, Muhlemann JK, Gayomba SR, Muday GK (2019) RBOH-dependent ROS synthesis and ROS scavenging by plant specialized metabolites to modulate plant development and stress responses. Chem Res Toxicol 32(3):370–396
Chini A, Fonseca SGDC, Fernandez G, Adie B, Chico J et al (2007) The JAZ family of repressors is the missing link in jasmonate signalling. Nature 448(7154):666–671
Cui LG, Shan JX, Shi M, Gao JP, Lin HX (2014) The miR156-SPL 9-DFR pathway coordinates the relationship between development and abiotic stress tolerance in plants. Plant J 80(6):1108–1117
Dai Z, Tan J, Zhou C, Yang X, Yang F, Zhang S, Sun S, Miao X, Shi Z (2019) The OsmiR396–Os GRF 8–OsF3H-flavonoid pathway mediates resistance to the brown planthopper in rice (Oryza sativa). Plant Biotechnol J 17(8):1657–1669
Davière JM, Achard P (2013) Gibberellin signaling in plants. Development 140(6):1147–1151
Erkan M, Wang SY, Wang CY (2008) Effect of UV treatment on antioxidant capacity, antioxidant enzyme activity and decay in strawberry fruit. Postharvest Biol Technol 48(2):163–171
Fan Di, Li C, Fan C, Jian Hu, Li J, Yao S, Wanxiang Lu, Yan Y, Luo K (2020) MicroRNA6443-mediated regulation of FERULATE 5-HYDROXYLASE gene alters lignin composition and enhances saccharification in Populus tomentosa. New Phytol 226(2):410–425
Feduraev P, Skrypnik L, Riabova A, Pungin A, Tokupova E, Maslennikov P, Chupakhina G (2020) Phenylalanine and tyrosine as exogenous precursors of wheat (Triticum aestivum L.) secondary metabolism through PAL-associated pathways. Plants 9(4):476
Feyissa BA, Arshad M, Gruber MY, Kohalmi SE, Hannoufa A (2019) The interplay between miR156/SPL13 and DFR/WD40–1 regulate drought tolerance in alfalfa. BMC Plant Biol 19(1):1–19
Fu H, Chao H, Zhao X, Wang H, Li H, Zhao W, Sun T, Li M, Huang J (2022) Anthocyanins identification and transcriptional regulation of anthocyanin biosynthesis in purple Brassica napus. Plant Mol Biol 110(1–2):53–68
Gao J, Chen B, Lin H, Liu Y, Wei Y, Chen F, Li W (2020) Identification and characterization of the glutathione S-Transferase (GST) family in radish reveals a likely role in anthocyanin biosynthesis and heavy metal stress tolerance. Gene 743:144484
Gisbert C, Dumm JM, Prohens J, Vilanova S, Stommel JR (2016) A spontaneous eggplant (Solanum melongena L.) colour mutant conditions anthocyanin-free fruit pigmentation. HortScience 51(7):793–798
Gou JY, Felippes FF, Liu CJ, Weigel D, Wang JW (2011) Negative regulation of anthocyanin biosynthesis in Arabidopsis by a miR156-targeted SPL transcription factor. Plant Cell 23(4):1512–1522
Gui J, Luo L, Zhong Y, Sun J, Umezawa T, Li L (2019) Phosphorylation of LTF1, an MYB transcription factor in Populus, acts as a sensory switch regulating lignin biosynthesis in wood cells. Mol Plant 12(10):1325–1337
Hawkesford MJ, Cakmak I, Coskun D, De Kok LJ, Lambers H, Schjoerring JK, White PJ (2023) Functions of macronutrients. Marschner’s mineral nutrition of plants. Academic press, Cambridge, pp 201–281
Hodges DM, Nozzolillo C (1996) Anthocyanin and anthocyanoplast content of cruciferous seedlings subjected to mineral nutrient deficiencies. J Plant Physiol 147(6):749–754
Huang W, Sun W, Lv H, Luo M, Zeng S, Pattanaik S (2013) A R2R3- MYB transcription factor from Epimedium sagittatum regulates the flavonoid biosynthetic pathway. PLoS ONE 8:e70778
Huang Q, Wang Y, Li B, Chang J, Chen M, Li K, Yang G, He G (2015) TaNAC29, a NAC transcription factor from wheat, enhances salt and drought tolerance in transgenic Arabidopsis. BMC Plant Biol 15:1–15
Huang W, Khaldun ABM, Lv H, Du L, Zhang C, Wang Y (2016) Isolation and functional characterization of a R2R3-MYB regulator of the anthocyanin biosynthetic pathway from Epimedium sagittatum. Plant Cell Rep 35:883–894
Imtiaz M, Mushtaq MA, Nawaz MA, Ashraf M, Rizwan MS, Mehmood S, Aziz O, Rizwan M, Virk MS, Shakeel Q, Ijaz R, Androutsopoulos VP, Tsatsakis AM, Coleman MD (2018) Physiological and anthocyanin biosynthesis genes response induced by vanadium stress in mustard genotypes with distinct photosynthetic activity. Environ Toxicol Pharmacol 62:20–29
Jaakola L (2013) New insights into the regulation of anthocyanin biosynthesis in fruits. Trends Plant Sci 18(9):477–483
Jahan MS, Guo S, Baloch AR, Sun J, Shu S, Wang Y, Ahammed GJ, Kabir K, Roy R (2020) Melatonin alleviates nickel phytotoxicity by improving photosynthesis, secondary metabolism and oxidative stress tolerance in tomato seedlings. Ecotoxicol Environ Saf 197:110593
Jan R, Asaf S, Numan M, Kim KM (2021) Plant secondary metabolite biosynthesis and transcriptional regulation in response to biotic and abiotic stress conditions. Agronomy 11(5):968
Jeandet P, Vannozzi A, Eduardo Sobarzo-Sánchez Md, Uddin S, Bru R, Martínez-Márquez A, Clément C, Cordelier S, Manayi A, Nabavi SF, Rasekhian M, Batiha G-S, Khan H, Morkunas I, Belwal T, Jiang J, Koffas M, Nabavi SM (2021) Phytostilbenes as agrochemicals: Biosynthesis, bioactivity, metabolic engineering and biotechnology. Nat Product Rep 38(7):1282–1329
Jiang M, Liu Y, Ren L, Lian H, Chen H (2016a) Molecular cloning and characterization of anthocyanin biosynthesis genes in eggplant (Solanum melongena L.). Acta Physiol Plant 38(7):1–13
Jiang M, Ren L, Lian H, Liu Y, Chen H (2016b) Novel insight into the mechanism underlying light-controlled anthocyanin accumulation in eggplant (Solanum melongena L.). Plant Sci 249:46–58
Jung CS, Griffiths HM, De Jong DM, Cheng S, Bodis M, Kim TS, De Jong WS (2009) The potato developer (D) locus encodes an R2R3 MYB transcription factor that regulates expression of multiple anthocyanin structural genes in tuber skin. Theor Appl Genet 120(1):45–57
Kadomura-Ishikawa Y, Miyawaki K, Takahashi A (2015) RNAi mediated silencing and overexpression of the FaMYB1 gene and its effect on anthocyanin accumulation in strawberry fruit. Biol Plantarum 59:677–685
Kapoor D, Singh MP, Kaur S, Bhardwaj R, Zheng B, Sharma A (2019) Modulation of the functional components of growth, photosynthesis, and anti-oxidant stress markers in cadmium exposed Brassica juncea L. Plants 8(8):260
Kee ES, Naing AH, Lim SH, Han JS, Kim CK (2016) MYB transcription factor isolated from Raphanus sativus enhances anthocyanin accumulation in chrysanthemum cultivars. 3 Biotech 6:79
Khandal H, Singh AP, Chattopadhyay D (2020) The MicroRNA397b-LACCASE2 module regulates root lignification under water and phosphate deficiency. Plant Physiol 182(3):1387–1403
Khoo HE, Azlan A, Tang ST, Lim SM (2017) Anthocyanidins and anthocyanins: coloured pigments as food, pharmaceutical ingredients, and the potential health benefits. Food Nutr Res 61(1):1361779
Kiferle C, Fantini E, Bassolino L, Povero G et al (2015) Tomato R2R3-MYB proteins SlANT1 and SlAN2: same protein activity, different roles. PLoS ONE 10(8):e0136365
Kim JI, Zhang X, Pascuzzi PE, Liu CJ, Chapple C (2020) Glucosinolate and phenylpropanoid biosynthesis are linked by proteasome-dependent degradation of PAL. New Phytol 225(1):154–168
Knudsen C, Gallage NJ, Hansen CC, Møller BL, Laursen T (2018) Dynamic metabolic solutions to the sessile life style of plants. Nat Prod Rep 35(11):1140–1155
Koeduka T, Sugimoto K, Watanabe B, Someya N, Kawanishi D, Gotoh T, Ozawa R, Takabayashi J, Matsui K, Hiratake J (2014) Bioactivity of natural O-prenylated phenylpropenes from I llicium anisatum leaves and their derivatives against spider mites and fungal pathogens. Plant Biol 16(2):451–456
Laursen T, Møller BL, Bassard JE (2015) Plasticity of specialized metabolism as mediated by dynamic metabolons. Trends Plant Sci 20(1):20–32
Lea US, Slimestad R, Smedvig P, Lillo C (2007) Nitrogen deficiency enhances expression of specific MYB and bHLH transcription factors and accumulation of end products in the flavonoid pathway. Planta 225(5):1245–1253
Le Roy J, Huss B, Creach A, Hawkins S, Neutelings G (2016) Glycosylation is a major regulator of phenylpropanoid availability and biological activity in plants. Front Plant Sci 7:735
Li P, Chen B, Zhang G, Chen L, Dong Q, Wen J et al (2016) Regulation of anthocyanin and proanthocyanidin biosynthesis by Medicago truncatula b HLH transcription factor M t TT 8. New Phytol 210(3):905–921
Li N, Wu H, Ding Q, Li H, Li Z, Ding J, Li Y (2018) The heterologous expression of Arabidopsis PAP2 induces anthocyanin accumulation and inhibits plant growth in tomato. Funct Integr Genomics 18(3):341–353
Li W, Tan L, Zou Y, Tan X, Huang J, Chen W, Tang Q (2020) The effects of ultraviolet A/B treatments on anthocyanin accumulation and gene expression in dark-purple tea cultivar ‘Ziyan’(Camellia sinensis). Molecules 25(2):354
Li X, Cao L, Jiao B, Yang H, Ma C, Liang Y (2022) The bHLH transcription factor AcB2 regulates anthocyanin biosynthesis in onion (Allium cepa L.). Hortic Res. https://doi.org/10.1093/hr/uhac128
Liao, H. –S., Yang, C. C., & Hsieh, M. H. (2022). Nitrogen deficiency-and sucrose-induced anthocyanin biosynthesis is modulated by HISTONE DEACETYLASE15 in Arabidopsis. J Exp Bot 73(11):3726–3742
Liou G (2019) Enzyme structure, function, and evolution in flavonoid biosynthesis (Doctoral dissertation, Massachusetts Institute of Technology)
Liu Y, Lin-Wang K, Deng C, Warran B, Wang L, Yu B et al (2015) Comparative transcriptome analysis of white and purple potato to identify genes involved in anthocyanin biosynthesis. PLoS ONE 10(6):e0129148
Liu Y, Lin-Wang K, Espley RV, Wang Li, Yang H, Bin Yu, Dare A, Varkonyi-Gasic E, Wang J, Zhang J, Wang Di, Allan AC (2016) Functional diversification of the potato R2R3 MYB anthocyanin activators AN1, MYBA1, and MYB113 and their interaction with basic helix-loop-helix cofactors. J Exp Bot 67(8):2159–2176
Liu Y, Hou H, Jiang X, Wang P, Dai X, Chen W, Gao L, Xia T (2018a) A WD40 repeat protein from Camellia sinensis regulates anthocyanin and proanthocyanidin accumulation through the formation of MYB–bHLH–WD40 ternary complexes. Int J Mol Sci 19(6):1686
Liu Y, Tikunov Y, Schouten RE, Marcelis LF, Visser RG, Bovy A (2018b) Anthocyanin biosynthesis and degradation mechanisms in Solanaceous vegetables: a review. Front Chem 6:52
Liu H, Liu Z, Wu Y, Zheng L, Zhang G (2021) Regulatory mechanisms of anthocyanin biosynthesis in apple and pear. Int J Mol Sci 22(16):8441
Liu D, Zhao D, Li X, Zeng Y (2022a) AtGLK2, an Arabidopsis GOLDEN2-LIKE transcription factor, positively regulates anthocyanin biosynthesis via AtHY5-mediated light signaling. Plant Growth Regul 96(1):79–90
Liu J, Wen B, Liu X, Yang Y, Li M, Wang X (2022b) Molecular and metabolic changes under environmental stresses: the biosynthesis of quality components in preharvest tea shoots. Horticulturae 8(2):173
Liu Y, Lv G, Yang Y, Ma K, Ren X, Li M, Liu Z (2022c) Interaction of AcMADS68 with transcription factors regulates anthocyanin biosynthesis in red-fleshed kiwifruit. Hortic Res 10:uhac252
Liu Y, Zhang X-W, Liu X, Zheng P-F, Ling Su, Wang G-L, Wang X-F, Li Y-Y, You C-X, An J-P (2022d) Phytochrome interacting factor MdPIF7 modulates anthocyanin biosynthesis and hypocotyl growth in apple. Plant Physiol 188(4):2342–2363
Lochmanová G, Zdráhal Z, Konečná H, Koukalová Š, Malbeck J et al (2008) Cytokinin-induced photomorphogenesis in dark-grown Arabidopsis: a proteomic analysis. J Exp Bot 59(13):3705–3719
Løvdal T, Olsen KM, Slimestad R, Verheul M, Lillo C (2010) Synergetic effects of nitrogen depletion, temperature, and light on the content of phenolic compounds and gene expression in leaves of tomato. Phytochemistry 71(5–6):605–613
Ma Y, Ma X, Gao X, Wu W, Zhou B (2021) Light induced regulation pathway of anthocyanin biosynthesis in plants. Int J Mol Sci 22(20):11116
Maes L, Inzé D, Goossens A (2008) Functional specialization of the TRANSPARENT TESTA GLABRA1 network allows differential hormonal control of laminal and marginal trichome initiation in Arabidopsis rosette leaves. Plant Physiol 148(3):1453–1464
Maier A, Hoecker U (2015) COP1/SPA ubiquitin ligase complexes repress anthocyanin accumulation under low light and high light conditions. Plant Signal Behav 10(1):e970440
Mazza G, Miniati E (2018) Anthocyanins in fruits, vegetables, and grains. CRC Press, Baco Raton
Mazzucato A, Willems D, Bernini R, Picarella ME, Santangelo E et al (2013) Novel phenotypes related to the breeding of purple-fruited tomatoes and effect of peel extracts on human cancer cell proliferation. Plant Physiol Biochem 72:125–133
Medina-Puche L, Cumplido-Laso G, Amil-Ruiz F, Hoffmann T, Ring L, Rodríguez-Franco A, Caballero JL, Schwab W, Muñoz-Blanco J, Blanco-Portales R (2014) MYB10 plays a major role in the regulation of flavonoid/phenylpropanoid metabolism during ripening of Fragaria × ananassa fruits. J Exp Bot 65:401–417
Meng X, Wang JR, Wang GD, Liang XQ, Li XD, Meng QW (2015) An R2R3-MYB gene, LeAN2, positively regulated the thermo-tolerance in transgenic tomato. J Plant Physiol 175:1–8
Meraj TA, Fu J, Raza MA, Zhu C, Shen Q, Xu D, Wang Q (2020) Transcriptional factors regulate plant stress responses through mediating secondary metabolism. Genes 11(4):346
Moustaka J, Tanou G, Giannakoula A, Adamakis IDS, Panteris E, Eleftheriou EP, Moustakas M (2020) Anthocyanin accumulation in poinsettia leaves and its functional role in photo-oxidative stress. Environ Exp Bot 175:104065
Movahed N, Pastore C, Cellini A, Allegro G, Valentini G et al (2016) The grapevine VviPrx31 peroxidase as a candidate gene involved in anthocyanin degradation in ripening berries under high temperature. J Plant Res 129(3):513–526
Naing AH, Kim CK (2018) Roles of R2R3-MYB transcription factors in transcriptional regulation of anthocyanin biosynthesis in horticultural plants. Plant Mol Biol 98:1–18
Nakabayashi R, Saito K (2015) Integrated metabolomics for abiotic stress responses in plants. Curr Opin Plant Biol 24:10–16
Ni J, Zhao Y, Tao R, Yin L, Gao L, Strid Å et al (2020) Ethylene mediates the branching of the jasmonate-induced flavonoid biosynthesis pathway by suppressing anthocyanin biosynthesis in red Chinese pear fruits. Plant Biotechnol J 18(5):1223–1240
Niu Y, Figueroa P, Browse J (2011) Characterization of JAZ-interacting bHLH transcription factors that regulate jasmonate responses in Arabidopsis. J Exp Bot 62(6):2143–2154
Niu J, Zhang G, Zhang W, Goltsev V, Sun S, Wang J et al (2017) Anthocyanin concentration depends on the counterbalance between its synthesis and degradation in plum fruit at high temperature. Sci Rep 7(1):1–16
Page M, Sultana N, Paszkiewicz K, Florance H, Smirnoff N (2012) The influence of ascorbate on anthocyanin accumulation during high light acclimation in Arabidopsis thaliana: further evidence for redox control of anthocyanin synthesis. Plant Cell Environ 35(2):388–404
Payyavula RS, Singh RK, Navarre DA (2013) Transcription factors, sucrose, and sucrose metabolic genes interact to regulate potato phenylpropanoid metabolism. J Exp Bot 64(16):5115–5131
Peng Y, Thrimawithana AH, Cooney JM, Jensen DJ, Espley RV, Allan AC (2020) The proanthocyanin-related transcription factors MYBC1 and WRKY44 regulate branch points in the kiwifruit anthocyanin pathway. Sci Rep 10(1):14161
Petrussa E, Braidot E, Zancani M, Peresson C, Bertolini A, Patui S, Vianello A (2013) Plant flavonoids—biosynthesis, transport and involvement in stress responses. Int J Mol Sci 14(7):14950–14973
Povero G, Gonzali S, Bassolino L, Mazzucato A, Perata P (2011) Transcriptional analysis in high-anthocyanin tomatoes reveals synergistic effect of Aft and atv genes. J Plant Physiol 168(3):270–279
Qian Y, Zhang T, Yu Y, Gou L, Yang J, Xu J, Pi E (2021) Regulatory mechanisms of bHLH transcription factors in plant adaptive responses to various abiotic stresses. Front Plant Sci 12:1143
Schreiber G, Reuveni M, Evenor D, Oren-Shamir M, Ovadia R et al (2012) ANTHOCYANIN1 from Solanum chilense is more efficient in accumulating anthocyanin metabolites than its Solanum lycopersicum counterpart in association with the ANTHOCYANIN FRUIT phenotype of tomato. Theor Appl Genet 124(2):295–307
Schwinn KE, Boase MR, Bradley JM, Lewis DH, Deroles SC, Martin CR, Davies KM (2014) MYB and bHLH transcription factor transgenes increase anthocyanin pigmentation in petunia and lisianthus plants, and the petunia phenotypes are strongly enhanced under field conditions. Front Plant Sci 5:603
Schwinn KE, Ngo H, Kenel F, Brummell DA, Albert NW, McCallum JA (2016) The onion (Allium cepa L.) R2R3-MYB gene MYB1 regulates anthocyanin biosynthesis. Front Plant Sci 7:1865
Sehar Z, Gautam H, Masood A, Khan NA (2022) Ethylene- and proline-dependent regulation of antioxidant enzymes to mitigate heat stress and boost photosynthetic efficacy in wheat plants. J Plant Growth Regul 42(5):2683–2697. https://doi.org/10.1007/s00344-022-10737-8
Sharma H, Chawla N, Dhatt AS (2019) Nutraceutical content and free radical scavenging capacity of brinjal (Solanum melongena L.) genotypes. Sci Hortic 244:294–303
Sharma H, Chawla N, Dhatt AS (2022) Role of phenylalanine/tyrosine ammonia lyase and anthocyanidin synthase enzymes for anthocyanin biosynthesis in developing Solanum melongena L. genotypes. Physiol Plant. https://doi.org/10.1111/ppl.13756
Sharma H, Chawla N, Dhatt AS (2023) Utilizing response surface methodology to optimize the extraction and characterization of anthocyanins from Solanum melongena L fruit. Nat Prod Res. https://doi.org/10.1080/14786419.2023.2244137
Shi L, Cao S, Chen W, Yang Z (2014) Blue light induced anthocyanin accumulation and expression of associated genes in Chinese bayberry fruit. Sci Hortic 179:98–102
Sinilal B, Ovadia R, Nissim-Levi A, Perl A, Carmeli-Weissberg M, Oren-Shamir M (2011) Increased accumulation and decreased catabolism of anthocyanins in red grape cell suspension culture following magnesium treatment. Planta 234:61–71
Stommel JR, Dumm JM (2015) Coordinated regulation of biosynthetic and regulatory genes coincides with anthocyanin accumulation in developing eggplant fruit. J Am Soc Hortic Sci 140(2):129–135
Stommel JR, Lightbourn GJ, Winkel BS, Griesbach RJ (2009) Transcription factor families regulate the anthocyanin biosynthetic pathway in Capsicum annuum. J Am Soc Hortic Sci 134(2):244–251
Stushnoff C, Ducreux LJ, Hancock RD, Hedley PE, Holm DG, McDougall GJ et al (2010) Flavonoid profiling and transcriptome analysis reveals new gene–metabolite correlations in tubers of Solanum tuberosum L. J Exp Bot 61(4):1225–1238
Su N, Wu Q, Liu Y, Cai J, Shen W, Xia K, Cui J (2014) Hydrogen-rich water reestablishes ROS homeostasis but exerts differential effects on anthocyanin synthesis in two varieties of radish sprouts under UV-A irradiation. J Agric Food Chem 62(27):6454–6462
Sulis DB, Wang JP (2020) Regulation of lignin biosynthesis by post-translational protein modifications. Front Plant Sci 11:914
Sun W, Zhou N, Feng C, Sun S, Tang M, Tang X, Ju Z, Yi Y (2021a) Functional analysis of a dihydroflavonol 4-reductase gene in Ophiorrhiza japonica (OjDFR1) reveals its role in the regulation of anthocyanin. PeerJ 9:e12323
Sun X, Zhang Q, Zhou H (2021b) Anthocyanins: from biosynthesis regulation to crop improvement. Botany Lett 168(4):546–557
Sun L, Huo J, Liu J, Jiayi Yu, Zhou J, Sun C, Wang Y, Leng F (2023) Anthocyanins distribution, transcriptional regulation, epigenetic and post-translational modification in fruits. Food Chem 411:135540
Symonds VV, Hatlestad G, Lloyd AM (2011) Natural allelic variation defines a role for ATMYC1: trichome cell fate determination. PLoS Genet 7(6):e1002069
Tao R, Bai S, Ni J, Yang Q, Zhao Y, Teng Y (2018) The blue light signal transduction pathway is involved in anthocyanin accumulation in ‘Red Zaosu’pear. Planta 248(1):37–48
Tohge T, de Souza LP, Fernie AR (2017) Current understanding of the pathways of flavonoid biosynthesis in model and crop plants. J Exp Bot 68(15):4013–4028
Tominaga-Wada R, Masakane A, Wada T (2018) Effect of phosphate deficiency-induced anthocyanin accumulation on the expression of Solanum lycopersicum GLABRA3 (SlGL3) in tomato. Plant Signal Behav 13(6):e1477907
Vojodi Mehrabani L, Kamran RV, Hassanpouraghdam MB, Pessarakli M (2017) Zinc sulfate foliar application effects on some physiological characteristics and phenolic and essential oil contents of Lavandula stoechas L. under sodium chloride (NaCl) salinity conditions. Commun Soil Sci Plant Anal 48(16):1860–1867
Wada T, Kunihiro A, Tominaga-Wada R (2014) Arabidopsis CAPRICE (MYB) and GLABRA3 (bHLH) control tomato (Solanum lycopersicum) anthocyanin biosynthesis. PLoS ONE 9(9):e109093
Wang H, Wang H (2015) The miR156/SPL module, a regulatory hub and versatile toolbox, gears up crops for enhanced agronomic traits. Mol Plant 8(5):677–688
Wang H, Wang C, Fan W, Yang J, Appelhagen I, Wu Y, Zhang P (2018a) A novel glycosyltransferase catalyses the transfer of glucose to glucosylated anthocyanins in purple sweet potato. J Exp Bot 69(22):5444–5459
Wang L, Liu N, Wang T, Li J, Wen T, Yang X, Lindsey K, Zhang X (2018b) The GhmiR157a–GhSPL10 regulatory module controls initial cellular dedifferentiation and callus proliferation in cotton by modulating ethylene-mediated flavonoid biosynthesis. J Exp Bot 69(5):1081–1093
Wu X, Gong Q, Ni X, Zhou Y, Gao Z (2017) UFGT: the key enzyme associated with the petals variegation in Japanese apricot. Front Plant Sci 8:108
Wu T, Liu HT, Zhao GP, Song JX, Wang XL et al (2020a) Jasmonate and ethylene-regulated ethylene response factor 22 promotes lanolin-induced anthocyanin biosynthesis in ‘Zaosu’pear (Pyrus bretschneideri Rehd.) fruit. Biomolecules 10(2):278
Wu X, Zhang S, Liu X, Shang J, Zhang A, Zhu Z, Zha D (2020b) Chalcone synthase (CHS) family members analysis from eggplant (Solanum melongena L.) in the flavonoid biosynthetic pathway and expression patterns in response to heat stress. PLoS ONE 15(4):e0226537
Xu F, Cao S, Shi L, Chen W, Su X, Yang Z (2014) Blue light irradiation affects anthocyanin content and enzyme activities involved in postharvest strawberry fruit. J Agric Food Chem 62(20):4778–4783
Xu W, Dubos C, Lepiniec L (2015) Transcriptional control of flavonoid biosynthesis by MYB–bHLH–WDR complexes. Trends Plant Sci 20(3):176–185
Xue C, Yao JL, Qin MF, Zhang MY, Allan AC, Wang DF, Wu J (2019) PbrmiR397a regulates lignification during stone cell development in pear fruit. Plant Biotechnol J 17(1):103–117
Yan Y, Stolz S, Chételat A, Reymond P, Pagni M, Dubugnon L, Farmer EE (2007) A downstream mediator in the growth repression limb of the jasmonate pathway. Plant Cell 19(8):2470–2483
Yang F, Cai J, Yang Y, Liu Z (2013) Overexpression of microRNA828 reduces anthocyanin accumulation in Arabidopsis. Plant Cell Tissue Organ Cult (PCTOC) 115:159–167
Yang T, Ma H, Zhang J, Wu T, Song T, Tian J, Yao Y (2019) Systematic identification of long noncoding RNA s expressed during light-induced anthocyanin accumulation in apple fruit. Plant J 100(3):572–590
Zhang X, Liu CJ (2015) Multifaceted regulations of gateway enzyme phenylalanine ammonia-lyase in the biosynthesis of phenylpropanoids. Mol Plant 8(1):17–27
Zhang Y, Butelli E, Martin C (2014) Engineering anthocyanin biosynthesis in plants. Curr Opin Plant Biol. https://doi.org/10.1016/j.pbi.2014.05.011
Zhang Y, Chu G, Hu Z, Gao Q, Cui B, Tian S (2016) Genetically engineered anthocyanin pathway for high health-promoting pigment production in eggplant. Mol Breed 36:54
Zhang J, Tuskan GA, Tschaplinski TJ, Muchero W, Chen JG (2020) Transcriptional and post-transcriptional regulation of lignin biosynthesis pathway genes in Populus. Front Plant Sci 11:652
Zhao H, Liu B, Zhang W, Cao J, Jiang W (2019a) Enhancement of quality and antioxidant metabolism of sweet cherry fruit by near-freezing temperature storage. Postharvest Biol Technol 147:113–122
Zhao Y, Zhang YY, Liu H, Zhang XS, Ni R et al (2019b) Functional characterization of a liverworts bHLH transcription factor involved in the regulation of bisbibenzyls and flavonoids biosynthesis. BMC Plant Biol 19(1):1–13
Zheng L, Chen Y, Ding D, Zhou Y, Ding L, Wei J, Wang H (2019) Endoplasmic reticulum–localized UBC34 interaction with lignin repressors MYB221 and MYB156 regulates the transactivity of the transcription factors in Populus tomentosa. BMC Plant Biol 19(1):1–17
Zhu Z, Li G, Liu L, Zhang Q, Han Z, Chen X, Li B (2018) A R2R3-MYB transcription factor, VvMYBC2L2, functions as a transcriptional repressor of anthocyanin biosynthesis in grapevine (Vitis vinifera L.). Molecules 24(1):92
Zong Y, Li G, Xi X, Sun X, Li S (2019a) A bHLH transcription factor TsMYC2 is associated with the blue grain character in triticale (Triticum × Secale). Plant Cell Rep 38(10):1291–1298
Zong Y, Li S, Xi X, Cao D, Wang Z, Wang R, Liu B (2019b) Comprehensive influences of overexpression of a MYB transcriptor regulating anthocyanin biosynthesis on transcriptome and metabolome of tobacco leaves. Int J Mol Sci 20(20):5123
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HS and NC conceptualized the idea; HS, PS, NC prepared the background information; HS, PS, AK drafted the manuscript, NC, ASD supervision; All authors have read and approved the manuscript.
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Sharma, H., Sharma, P., Kumar, A. et al. Multifaceted Regulation of Anthocyanin Biosynthesis in Plants: A Comprehensive Review. J Plant Growth Regul (2024). https://doi.org/10.1007/s00344-024-11306-x
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DOI: https://doi.org/10.1007/s00344-024-11306-x