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The Production of Cephalosporin C by Acremonium chrysogenum is Improved by the Intracellular Expression of a Bacterial Hemoglobin

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Abstract

A DNA vector for expressing an oxygen-binding heme protein (Vitreoscilla hemoglobin, or VHb) in filamentous fungi was constructed and introduced into a cephalosporin C-producing strain of Acremonium chrysogenum. Expression of VHb in transformants was demonstrated by Western immunoblot analysis and by increased carbon monoxide binding activity of cell extracts. Several VHb-expressing transformants produced significantly higher yields of cephalosporin C than control strains in batch culture experiments. Using the same vector system, VHb was also expressed in the related fungus Penicillium chrysogenum.

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Author notes

  1. D. E. Hughes: Corresponding author.

Authors and Affiliations

  1. Exogene Corporation, 222 E. Huntington Drive, Suite 111, Monrovia, CA, 91016

    J. A. DeModena, R. A. Fachini, J. L. Galazzo & D. E. Hughes

  2. Department of Microbiology, University of León, León, Spain

    S. Gutiérrez, J. Velasco, F. J. Fernández & J. F. Martin

Authors
  1. J. A. DeModena
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  2. S. Gutiérrez
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  3. J. Velasco
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  4. F. J. Fernández
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  5. R. A. Fachini
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  6. J. L. Galazzo
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  7. D. E. Hughes
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  8. J. F. Martin
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DeModena, J., Gutiérrez, S., Velasco, J. et al. The Production of Cephalosporin C by Acremonium chrysogenum is Improved by the Intracellular Expression of a Bacterial Hemoglobin. Nat Biotechnol 11, 926–929 (1993). https://doi.org/10.1038/nbt0893-926

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  • DOI: https://doi.org/10.1038/nbt0893-926

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