Summary
Cephalosporin C was produced with the moldCephalosporium acremonium in a 20 1 stirred tank reactor with 100 kg/m3 peanut flour in fed-batch operation. The growth and product formation was followed by on-line analysis of the broth composition. The cell concentration was estimated from the RNA-content of the cells. By optimization of the fed-batch operation and by increasing the phosphate content in the broth, a final cephalosporin C concentration of 12 kg/m3 was attained.
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Abbreviations
- CPC:
-
cephalosphorin C
- DAC:
-
deacetylcephalosporin C
- DAOC:
-
deacetoxycephalosporin C
- k L a :
-
volumetric mass transfer coefficient
- MMBS:
-
2-Hydroxy-4-methylmercaptobutyric acid
- PABAH:
-
p-Hydroxybenzoicacidhydrazid
- RNA:
-
ribonucleic acid
- RQ:
-
respiratory quotient
- \(Q_{O_2 } \) :
-
oxygen transfer rate
- \(Q_{CO_2 } \) :
-
CO2-production rate
- t :
-
fermentation time
References
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Herold, T., Bayer, T. & Schügerl, K. Cephalosporin C production in a stirred tank reactor. Appl Microbiol Biotechnol 29, 168–173 (1988). https://doi.org/10.1007/BF00939302
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DOI: https://doi.org/10.1007/BF00939302