Summary
The behaviour and applicability of several controllers for maintaining a constant dissolved oxygen concentration (DO) during the cephalosporin C production with Cephalosporium acremonium in a laboratory fermentor is described. The process controllers were realized on a MC 68000 based process computer using the real-time language PEARL. The discrete signum integral controller showed the best control action. In addition some derived fermentation data were calculated on-line by the process computer.
The results obtained by comparison of fermentations carried out at DO between 10% and 40% saturation during ideophase indicate that high DO leads to a high specific production rate for cephalosporin C and a low specific production rate for penicillin N and vice versa. In the range of DO investigated the production of deacetyl and deacetoxy cephalosporin C is not affected by DO. A direct correlation between DO and the yield coefficients Y P/S and Y P/X could be established. The yield coefficient Y P/O for cephalosporin C is constant in the DO range from 10%–40%.
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Dedicated to Prof. Dr. H. J. Rehm on the occasion of his 60th birthday
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Hilgendorf, P., Heiser, V., Diekmann, H. et al. Constant dissolved oxygen concentrations in cephalosporin C fermentation: Applicability of different controllers and effect on fermentation parameters. Appl Microbiol Biotechnol 27, 247–251 (1987). https://doi.org/10.1007/BF00252926
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DOI: https://doi.org/10.1007/BF00252926