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What is flux balance analysis?

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Flux balance analysis is a mathematical approach for analyzing the flow of metabolites through a metabolic network. This primer covers the theoretical basis of the approach, several practical examples and a software toolbox for performing the calculations.

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Figure 1: The conceptual basis of constraint-based modeling.
Figure 2: Formulation of an FBA problem.

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Acknowledgements

This work was supported by National Institutes of Health grant no. R01 GM057089.

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Authors and Affiliations

  1. Jeffrey D. Orth and Bernhard Ø. Palsson are at the University of California San Diego, La Jolla, California, USA.,

    Jeffrey D Orth & Bernhard Ø Palsson

  2. Ines Thiele is at the University of Iceland, Reykjavik, Iceland.,

    Ines Thiele

Authors
  1. Jeffrey D Orth
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  2. Ines Thiele
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  3. Bernhard Ø Palsson
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Correspondence to Bernhard Ø Palsson.

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Orth, J., Thiele, I. & Palsson, B. What is flux balance analysis?. Nat Biotechnol 28, 245–248 (2010). https://doi.org/10.1038/nbt.1614

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