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Updated structure of Drosophila cryptochrome

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Abstract

Arising from B. D. Zoltowski et al. Nature 480, 396–399 (2011)10.1038/nature10618

Recently, we determined the X-ray crystal structure of full-length cryptochrome from Drosophila1. Here we report an improved model of the Drosophila cryptochrome (dCRY) structure that corrects errors in the original coordinates (Protein Data Bank (PDB) accession 3TVS). Further refinement of the structure, with automated rebuilding algorithms in Phenix2 followed by manual building, indicated that a model of dCRY could be produced with excellent refinement statistics without taking into account the non-merohedral twinning originally reported (Table 1).

Table 1 Data collection and phasing statistics
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Figure 1: Superposition of Cα traces for 3TVS and rebuilt dCRY structure.
Figure 2: Sequence shift in the CTT.
Figure 3: Flavin centre of dCRY.
Figure 4: dCRY Trp triad photoreduction pathway.

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Accession codes

Primary accessions

Protein Data Bank

References

  1. Zoltowski, B. D. et al. Structure of full-length Drosophila cryptochrome. Nature 480, 396–399 (2011)

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  2. Afonine, P. V., Grosse-Kunstieve, R. W. & Adams, P. D. The Phenix refinement framework. CCP4 Newslett. Number 42, contribution 8. (2005)

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Authors and Affiliations

  1. *Manchester Institute of Biotechnology, Faculty of Life Sciences, University of Manchester, 131 Princess Street, Manchester M1 7DN, UK,

    Colin Levy, Alex R. Jones, Nigel S. Scrutton & David Leys

  2. Department of Chemistry and Chemical Biology, Cornell University, Ithaca, 14853, New York, USA

    Brian D. Zoltowski, Anand T. Vaidya, Joanne Widom & Brian R. Crane

  3. Laboratory of Genetics, The Rockefeller University, New York, 10065, New York, USA

    Deniz Top & Michael W. Young

Authors
  1. Colin Levy
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  2. Brian D. Zoltowski
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  3. Alex R. Jones
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  4. Anand T. Vaidya
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  5. Deniz Top
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  6. Joanne Widom
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  7. Michael W. Young
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  8. Nigel S. Scrutton
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  9. Brian R. Crane
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  10. David Leys
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Contributions

The New York authors (B.D.Z., A.T.V., D.T., J.W., M.W.Y. and B.R.C.) carried out the work presented in ref. 1; the Manchester authors (C.L., A.R.J., N.S.S. and D.L.) identified errors in the original coordinates; together, both sets of authors carried out the further refinement of the structure presented here. C.L. initially identified errors in the original coordinates, and C.L. and D.L. performed initial model rebuilding and crystallographic analysis, which was verified and discussed with A.R.J. and N.S.S. The New York authors B.D.Z. and B.R.C. completed and verified the model rebuilding and crystallographic analysis. The New York authors A.T.V., D.T., J.W. and M.W.Y. contributed to structural analysis and verified the new structure against existing biochemical and biological data. B.R.C. and B.D.Z. wrote the communication with input from all other authors.

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Correspondence to Brian R. Crane.

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Levy, C., Zoltowski, B., Jones, A. et al. Updated structure of Drosophila cryptochrome. Nature 495, E3–E4 (2013). https://doi.org/10.1038/nature11995

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