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Crystallization and Preliminary X-Ray Diffraction Analysis of the ZAD Domain of the Serendipity-d Protein from Drosophila melanogaster

  • STRUCTURE OF MACROMOLECULAR COMPOUNDS
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Abstract

The spatial organization of the genome is controlled by a special class of architectural proteins, in particular proteins containing ZAD domains that are able to form homo- and heterodimers. These domains are unique for arthropods and are present at the N-terminus of many proteins containing C2H2-type zinc fingers. Despite a growing interest in the characterization of architectural proteins, little is known about their structures (structures of their functional domains), which could shed light on the mechanisms of regulatory processes of the establishment and maintenance of the spatial organization of chromatin domains. Only two structures of ZAD domains are known. Here we report the crystallization and preliminary X-ray diffraction analysis of the previously unknown ZAD domain of the Serendipity-d protein from Drosophila melanogaster.

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REFERENCES

  1. Q. Szabo, F. Bantignies, and G. Cavalli, Sci. Adv. 5 (2019). https://doi.org/10.1126/sciadv.aaw1668

  2. J. Dekker and L. Mirny, Cell 164, 1110 (2016).

    Article  Google Scholar 

  3. O. Maksimenko and P. Georgiev, Front. Genet. 5, 28 (2014).

    Article  Google Scholar 

  4. O. Maksimenko, O. Kyrchanova, N. Klimenko, et al., Biochim. Biophys. Acta Gene Regul Mech. 1863, 194446 (2020).

    Article  Google Scholar 

  5. N. Zolotarev, O. Maksimenko, O. Kyrchanova, et al., Nucleic Acids Res. 45, 12285 (2017).

    Article  Google Scholar 

  6. A. A. Fedotova, A. N. Bonchuk, V. A. Mogila, et al., Acta Nat. 9, 47 (2017).

    Article  Google Scholar 

  7. H. Zheng and W. Xie, Nat. Rev. Mol. Cell. Biol. 20, 535 (2019).

    Article  Google Scholar 

  8. N. Zolotarev, A. Fedotova, O. Kyrchanova, et al., Nucleic Acids Res. 44, 7228 (2016).

    Google Scholar 

  9. K. M. Boyko, A. V. Lipkin, V. O. Popov, et al., Crystallogr. Rep. 58, 442 (2013).

    Article  ADS  Google Scholar 

  10. Z. Otwinowski and W. Minor, Macromol. Crystallogr. A 276, 307 (1997).

    Article  Google Scholar 

  11. T. G. G. Battye, L. Kontogiannis, O. Johnson, et al., Acta Crystallogr. D 67, 271 (2011).

    Article  Google Scholar 

  12. B. W. Matthews, J. Mol. Biol. 33, 491 (1968).

    Article  Google Scholar 

  13. M. D. Winn, C. C. Ballard, K. D. Cowtan, et al., Acta Crystallogr. D 67, 235 (2011).

    Article  Google Scholar 

  14. A. A. Vagin and M. N. Isupov, Acta Crystallogr. D 57, 1451 (2001).

    Article  Google Scholar 

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Funding

The study was supported by the Russian Science Foundation (project no. 19-74-10099 supporting the protein expression and isolation), the Russian Federal Space Agency (project of Space experiments (target works) “Kristallizator” supporting the crystallization and X-ray diffraction data collection), and the Ministry of Science and Higher Education of the Russian Federation (analysis of the results).

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Authors and Affiliations

  1. Bach Institute of Biochemistry, Federal Research Centre “Fundamentals of Biotechnology,” Russian Academy of Sciences, 119071, Moscow, Russia

    K. M. Boyko & V. O. Popov

  2. National Research Centre “Kurchatov Institute”, 123098, Moscow, Russia

    A. Yu. Nikolaeva & V. O. Popov

  3. Institute of Gene Biology, Russian Academy of Sciences, 119334, Moscow, Russia

    A. N. Bonchuk & P. G. Georgiev

Authors
  1. K. M. Boyko
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  2. A. Yu. Nikolaeva
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  3. A. N. Bonchuk
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  4. P. G. Georgiev
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  5. V. O. Popov
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Corresponding author

Correspondence to K. M. Boyko.

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Translated by T. Safonova

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Boyko, K.M., Nikolaeva, A.Y., Bonchuk, A.N. et al. Crystallization and Preliminary X-Ray Diffraction Analysis of the ZAD Domain of the Serendipity-d Protein from Drosophila melanogaster. Crystallogr. Rep. 65, 593–595 (2020). https://doi.org/10.1134/S1063774520040057

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  • DOI: https://doi.org/10.1134/S1063774520040057

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