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Analysis of heteroantisera to cells from human malignant effusions by immunofluorescence and protein A binding

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Abstract

Using cultured cells derived from human malignant effusions, hetero-antisera were raised in rabbits. The antisera were sequentially absorbed on various human non-tumour cells, reactivity being monitored by immunofluorescence and 125I-labelled staphylococcal protein A assays. The absorbed antisera possessed common reactivity to all tumour cells assayed. This reactivity was not histogenically determined, and our data suggest that it was not directed to oncofoetal antigens.

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Authors
  1. J P Birchall
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  2. J J Owen
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  3. B S Owen
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Birchall, J., Owen, J. & Owen, B. Analysis of heteroantisera to cells from human malignant effusions by immunofluorescence and protein A binding. Br J Cancer 36, 730–742 (1977). https://doi.org/10.1038/bjc.1977.256

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  • DOI: https://doi.org/10.1038/bjc.1977.256

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