Abstract
Rabbit hybridomas are gaining wide acceptance as serologic reagents to identify a variety of antigens, including proteins, small peptides, phosphorylated proteins, and polysaccharides. Rabbits make high-affinity IgG antibodies, all of which bind with high affinity to Protein A from Staphylococcus aureus and Protein G from Group G Streptococcus. Consequently, rabbit monoclonal antibodies of desired specificity can be rapidly detected using Protein A/G as secondary reagents. Here we describe the method for generating rabbit monoclonal antibodies using the rabbit hybridoma fusion partner 240E-1. The method begins with the immunization of rabbits and ends with the cloning the antigen-specific hybridomas.
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Yam, PC., Knight, K.L. (2014). Generation of Rabbit Monoclonal Antibodies. In: Ossipow, V., Fischer, N. (eds) Monoclonal Antibodies. Methods in Molecular Biology, vol 1131. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-992-5_5
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DOI: https://doi.org/10.1007/978-1-62703-992-5_5
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-991-8
Online ISBN: 978-1-62703-992-5
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