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Light-inducible and chloroplast-associated expression of a chimaeric gene introduced into Nicotiana tabacum using a Ti plasmid vector

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Abstract

A chimaeric gene, consisting of the 5′-flanking region of a member of the Pisum sativum gene family encoding ribulose 1,5-bisphosphate carboxylase linked to the coding region of a bacterial chloramphenicol acetyltransferase gene, has been introduced into the genome of the plant Nicotiana tabacum using a Ti plasmid of Agrobacterium tumefaciens. The expression of the chimaeric gene is light-inducible in chloroplast-containing transformed tissue.

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Authors and Affiliations

  1. Laboratorium voor Genetica, Rijksuniversiteit Gent, Gent, B-9000, Belgium

    L. Herrera-Estrella, G. Van den Broeck, R. Maenhaut, M. Van Montagu & J. Schell

  2. Laboratory of Cell Biology, The Rockefeller University, New York, New York, 10021, USA

    M. Timko & A. Cashmore

Authors
  1. L. Herrera-Estrella
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  2. G. Van den Broeck
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  3. R. Maenhaut
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  4. M. Van Montagu
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  5. J. Schell
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  6. M. Timko
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  7. A. Cashmore
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Herrera-Estrella, L., Van den Broeck, G., Maenhaut, R. et al. Light-inducible and chloroplast-associated expression of a chimaeric gene introduced into Nicotiana tabacum using a Ti plasmid vector. Nature 310, 115–120 (1984). https://doi.org/10.1038/310115a0

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  • DOI: https://doi.org/10.1038/310115a0

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