Abstract
Polyadenylated RNA from leaves of pea (Pisum sativum) has been copied into DNA and cloned in the Escherichia coli plasmid pBR322. From these clones we have identified and sequenced DNA encoding the mRNA for the precursor of the small subunit of the chloroplast enzyme ribulose-1,5-bisphosphate carboxylase.
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Ellis, R. J., Smith, S. M. & Barraclough, B. R. in Genome Organisation and Expression in Plants (ed. Leaver, C. J.) 321–335 (Plenum, New York, 1980).
Chua, N-H. & Schmidt, G. W. J. Cell Biol. 81, 461–483 (1979).
Ellis, R. J. Trends biochem. Sci. 4, 241–244 (1979).
Jensen, R. G. & Bahr, J. T. A. Rev. Pl. Physiol. 28, 379–400 (1977).
Coen, D. M., Bedbrook, J. R., Bogorad, L. & Rich, A. Proc. natn. Acad. Sci. U.S.A. 74, 5487–5491 (1977).
Bedbrook, J. R., Coen, D. M., Beaton, A. R., Bogorad, L. & Rich, A. J. biol. Chem. 254, 905–910 (1977).
Blair, G. E. & Ellis, R. J. Biochim. biophys. Acta 319, 223–234 (1973).
Kawashima, N. & Wildman, S. G. Biochim. biophys. Acta 262, 42–49 (1972).
Dobberstein, B., Blobel, G. & Chua, N. H. Proc. natn. Acad. Sci. U.S.A. 74, 1082–1085 (1977).
Highfield, P. E. & Ellis, R. J. Nature 271, 420–424 (1978).
Chua, N. H. & Schmidt, G. W. Proc. natn. Acad. Sci. U.S.A. 75, 6110–6114 (1978).
Smith, S. M. & Ellis, R. J. Nature 278, 662–664 (1979).
Blobel, G. & Dobberstein, B. J. Cell Biol. 67, 835–851 (1975).
Link, G., Coen, D. M. & Bogorad, L. Cell 15, 725–731 (1978).
Grunstein, M. & Hogness, D. S. Proc. natn. Acad. Sci. U.S.A. 72, 3961–3965 (1975).
Smith, D. F., Searle, P. F. & Williams, J. G. Nucleic Acids Res. 6, 487–506 (1979).
Maxam, A. M. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).
Bedbrook, J. & Bogorad, L. Proc. natn. Acad. Sci. U.S.A. 73, 4309–4313 (1976).
Smith, H. O. & Birnstiel, M. L. Nucleic Acids Res. 3, 2387–2398 (1976).
Martin, P. G. Aust. J. Pl. Physiol. 6, 401–408 (1979).
Schmidt, G. W., Devilliers-Thiery, A., Desruisseaux, H., Blobel, G. & Chua, N.-H. J. Cell Biol. 83, 615–622 (1979).
Cheng, C. C. et al. J. molec. Biol. 107, 527–547 (1976).
McMaster, G. K. & Carmichael, G. G. Proc. natn. Acad. Sci. U.S.A. 74, 4835–4838 (1977).
Alwine, J. C., Kemp, D. J. & Stark, G. R. Proc. natn. Acad. Sci. U.S.A. 74, 5350–5354 (1977).
Austen, B. M. FEBS Lett. 103, 308–313 (1977).
Loening, U. E. Biochem. J. 113, 131–138 (1969).
Myers, J. C. & Spiegelman, S. Proc. natn. Acad. Sci. U.S.A. 75, 5329–5333 (1978).
Boyer, H. W. et al. in Recombinant Molecules; Impact on Science and Society (eds Beers, R. F. & Bassett, E. G.) (Raven, New York, 1977).
Boyer, H. W. & Roulland-Dossaix, D. J. molec. Biol. 41, 459–472 (1969).
Roychoudhury, R., Jay, E. & Wu, R. Nucleic Acids Res. 3, 101–116 (1976).
Maizels, N. Cell 9, 431–438 (1976).
Clewell, D. B. J. Bact. 110, 667–676 (1972).
Dingman, C. W. & Peacock, A. C. Biochemistry 7, 659–668 (1968).
Rigby, P. W. J., Dieckmann, M., Rhodes, C. & Berg, P. J. molec. Biol. 113, 237–251 (1977).
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Bedbrook, J., Smith, S. & Ellis, R. Molecular cloning and sequencing of cDNA encoding the precursor to the small subunit of chloroplast ribulose-1,5-bisphosphate carboxylase. Nature 287, 692–697 (1980). https://doi.org/10.1038/287692a0
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DOI: https://doi.org/10.1038/287692a0
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