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Plastid genome of Seseli montanum: Complete sequence and comparison with plastomes of other members of the Apiaceae family

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Abstract

This work reports the complete plastid (pt) DNA sequence of Seseli montanum L. of the Apiaceae family, determined using next-generation sequencing technology. The complete genome sequence has been deposited in GenBank with accession No. KM035851. The S. montanum plastome is 147,823 bp in length. The plastid genome has a typical structure for angiosperms and contains a large single-copy region (LSC) of 92,620 bp and a small single-copy region (SSC) of 17,481 bp separated by a pair of 18,861 bp inverted repeats (IRa and IRb). The composition, gene order, and AT-content in the S. montanum plastome are similar to that of a typical flowering plant pt DNA. One hundred fourteen unique genes have been identified, including 30 tRNA genes, four rRNA genes, and 80 protein genes. Of 18 intron-containing genes found, 16 genes have one intron, and two genes (ycf3, clpP) have two introns. Comparative analysis of Apiaceae plastomes reveals in the S. montanum plastome a LSC/IRb junction shift, so that the part of the ycf2 (4980 bp) gene is located in the LSC, but the other part of ycf2 (1301 bp) is within the inverted repeat. Thus, structural rearrangements in the plastid genome of S. montanum result in an enlargement of the LSC region by means of capture of a large part of ycf2, in contrast to eight Apiaceae plastomes where the complete ycf2 gene sequence is located in the inverted repeat.

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Abbreviations

CTAB:

cetyltrimethylammonium bromide

IR:

inverted repeat

JL:

junction between LSC and IR

JS:

junction between SSC and IR

LSC:

large single-copy region

SSC:

small single-copy region

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Authors and Affiliations

  1. Lomonosov Moscow State University, Belozersky Institute of Physico-Chemical Biology, 119991, Moscow, Russia

    T. H. Samigullin, M. D. Logacheva & C. M. Vallejo-Roman

  2. Lomonosov Moscow State University, Biological Faculty, 119991, Moscow, Russia

    E. I. Terenteva & G. V. Degtjareva

Authors
  1. T. H. Samigullin
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  2. M. D. Logacheva
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  3. E. I. Terenteva
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  4. G. V. Degtjareva
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  5. C. M. Vallejo-Roman
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Correspondence to T. H. Samigullin.

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Original Russian Text © T. H. Samigullin, M. D. Logacheva, E. I. Terenteva, G. V. Degtjareva, C. M. Vallejo-Roman, 2016, published in Biokhimiya, 2016, Vol. 81, No. 9, pp. 1232-1238. Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM16-142, August 8, 2016.

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Samigullin, T.H., Logacheva, M.D., Terenteva, E.I. et al. Plastid genome of Seseli montanum: Complete sequence and comparison with plastomes of other members of the Apiaceae family. Biochemistry Moscow 81, 981–985 (2016). https://doi.org/10.1134/S0006297916090078

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  • DOI: https://doi.org/10.1134/S0006297916090078

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