Determination of Type-specific Antibodies against Poliovirus labelled with Phosphorus-32 by an Elution Test

Abstract

IT has been demonstrated that type 1 poliovirus strains Mahoney and LSc, 2ab may be differentiated by their elution pattern on certain adsorbents. Aluminium hydroxide-gel proved to be a very suitable substance in this respect. Strain Mahoney is readily eluted by 0.01 M phosphate buffer pH. 7.5, whereas strain LSc, 2ab of the same serological type requires 30–40-fold higher concentrations of phosphate to elute quantitatively with a sharp peak^1–3. This phenomenon of different elution pattern may be an expression of different surface properties of these strains of virus. This leads to the question whether the specific behaviour of the strains is altered if incubation with type specific antibody precedes the elution procedure.