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Gel Filtration as a Method for Purification of Protein-bound Peptides Exemplified by Oxytocin and Vasopressin

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Abstract

WE would like to call attention to a new procedure suitable for purification of simple compounds weakly associated with high molecular weight substances. The method, which was proposed elsewhere by one of us1, is based on separation according to molecular size achievable by means of filtration through dextran gels2. The principle which involves three operations may be illustrated for protein-bound peptides by the following scheme :

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References

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Authors and Affiliations

  1. Institute of Biochemistry, Uppsala

    E. B. LINDNER, A. ELMQVIST & J. PORATH

  2. State Pharmaceutical Laboratory, Stockholm

    E. B. LINDNER, A. ELMQVIST & J. PORATH

Authors
  1. E. B. LINDNER
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  2. A. ELMQVIST
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  3. J. PORATH
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LINDNER, E., ELMQVIST, A. & PORATH, J. Gel Filtration as a Method for Purification of Protein-bound Peptides Exemplified by Oxytocin and Vasopressin. Nature 184, 1565–1566 (1959). https://doi.org/10.1038/1841565b0

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