Abstract
GORDON, MARTIN AND SYNGE1 and Consden, Gordon and Martin2 have shown that good separations of amino-acids can be obtained on filter paper by allowing a suitable solvent, which has previously been saturated with water, to flow over the paper in a closed container, the air in which is saturated with the vapours of water and the solvent. Filter paper contains 20–25 per cent water under these conditions, and separation depends upon the differences in partition coefficient of the amino-acids between the stationary water phase and the moving solvent. It has further been shown2 that for any individual amino-acid the value
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References
Gordon, A. H., Martin, A. J. P., and Synge, R. L. M., Biochem. J., 37, Proc. xiii (1943).
Consden, R., Gordon, A. H., and Martin, A. J. P., Biochem. J., 38, 224 (1944).
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PARTRIDGE, S. Application of the Paper Partition Chromatogram to the Qualitative Analysis of Reducing Sugars. Nature 158, 270–271 (1946). https://doi.org/10.1038/158270a0
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DOI: https://doi.org/10.1038/158270a0
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