Skip to main content
Log in

Culture of nonfertilized female gametophytesin vitro

  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

This is a review of literature devoted to in vitro culture of nonfertilized ovaries and ovules. Attention is given to the possibility of induction of haploid plants from female gametophyte culture through analysis of factors affecting the processes of morphogenesis in ovaries and ovules culture. Induction of haploid development depends on genotype, stage of female gametophyte development, composition of nutrient media and conditions of cultivation. A cytoembryological picture of in vitro development of the female gametophyte is given. Egg cells, synergids and antipodals are also capable of inducing embryogenesis or callus formation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  • Ao GS, Zhao S & Li G (1982) In vitro induction of haploid plantlets from unpollinated ovaries of corn (Zea mays L.). Acta Genetica Sinica. 9: 281–283

    Google Scholar 

  • Abugaliv IA & Kozhahmetov MK (1993) Use of in vitro methods in sugar beet breeding. Doklady Rossiiskoi Akademii Selskokhozyaistvennykh Nauk. 3: 8–11

    Google Scholar 

  • Alatorceva TA & Tyrnov VS (1994) Comparison of development of unpollinated ovaries of apo-and amphimictic lines of corn in vitro. Apomixis u rastenii: Sostoyanie problemy i perspektivy. Proc. Int. Symp. (pp 8–9). Saratov

  • Beaville MA (1980) Obtention d'haploides in vitro a partir d' ovaries non fecondes de Riz (Oriza sativa L.). C.R.Acad. Sci. 290: 489–492

    Google Scholar 

  • Belous VE, Berdyshev AG, Oleynyk NA & Perfilyeva LP (1990) Sugar beet embryos development during cultivation of isolated nonpolllinated ovules in vitro. Embryol. & Seed Reprod. Proc. XI Int. Symp. (pp 19). Leningrad

  • Bohanec B, Javornik B, Bohanec B & Kreft I (1994) Induction of gynogenesis in agricultural crops: a review. In: Bohanec B & Javornik B (ed) Proc. Int. Colloquium Plant Biotechnology on Agriculture (pp 43–55), Ljubljana, Slovenia

  • Bossoutrot D & Hosemans D (1985) Gynogenesis in Beta vulgaris L.: From in vitro culture to the production of doubled haploids plants in soil. Plant Cell Repts. 4: 300–303

    Article  Google Scholar 

  • Bugara AM & Rusina LV (1989) Haploid callusogenesis from culture of nonfertilized ovules of sage. Physiologiya i Biohimiya Culturhyh Rastenii. 21: 554–560

    Google Scholar 

  • Campion B & Alloni C (1990) Induction of haploid plants in onion (Allium cepa L.) by in vitro culture unpollinated ovules. Plant Cell Tiss. Org. Cult. 20: 1–6

    Article  CAS  Google Scholar 

  • Campion B, Perri E, Azzimonti MT, Vicini E & Schiavi (1995) Spontaneous and induced chromosome doubling in gynogenic lines of onion (Allium cepa L.). Plant Breeding. 114: 243–246

    Article  Google Scholar 

  • Castillo AM & Cistue L (1993) Production of gynogenetic haploids of Hordeum vulgare L. Plant Cell Repts. 12: 139–143

    CAS  Google Scholar 

  • Chopra RN (1958) In vitro culture of ovaries of Althaea rosea Cav. In: Maheshwari P (ed) Proc. Seminar Mod. Dev. Plant Physiol (pp 87–89). Delhi

  • Chu CC (1978) The N6 medium and its application to anther culture of cereal crops. Proc. Symp. Plant Tissue Culture. Science Press (pp 43–50). Beiging

  • Choob VV, Vlasova TA & Butenko RG (1994) Callusogenesis and morphogenesis in generative organ culture of the spring-flowering species of Crocus L. Russian J. Plant Physiology. 41: 712–716

    Google Scholar 

  • Davojan EI (1985) Obtaining rice haploids from unpollinated ovaries culture technique in vitro conditions. Doklady VASHNIL. 3: 15–16

    Google Scholar 

  • Dryanovsca OA (1985) Induced callus in vitro from ovaries and anthers of species from the Cucurbitaceae family. Ibid. 9: 1243–1244

    Google Scholar 

  • Dryanovsca OA & Ilieva IN (1983) In vitro anther and ovule cultures in muskmelon (Cucumis melo L.). C. Acad. Bulg. Sci. 36: 1107–1110

    Google Scholar 

  • Erezhepov AE, Mukhambetzhanov SK, Kolumbaeva SZh, Azimova ED & Omirbekova ND (1991) Culture of unfertilized wheat ovaries: effect of genotype and phytohormones. Aktualnye Problemy Sovremennoi Biologii (pp 23–24). Alma-Ata, Kazakhstan

    Google Scholar 

  • Ferrant V & Bouharmont J (1994) Origin of gynogenetic embryos of Beta vulgaris L. Sexual Plant Reproduction. 7: 12–16

    Article  Google Scholar 

  • Ferrie AMR, Palmer CE, Keller WA & Thorpe TA (1995) Haploid embryogenesis. In: In vitro embryogenesis in plants (pp 309–344). Kluwer Acad. Publishers, Netherlands

    Google Scholar 

  • Fodale As, Mule R, Parlati MV & Tucci A (1992) Preliminary studies on the in vitro ovule culture of Olea europeae L. Agricoltura Ricerca. 14: 63–68

    Google Scholar 

  • Gamborg OL & Eveleigh DE (1968) Culture methods and detection of glucanases in cultures of wheat and barley. Can. J. Biochem. 46: 417–421

    Article  PubMed  CAS  Google Scholar 

  • Garcia-Martinez JL & Carbonel J (1985) Induction on fruit set and development in pea ovary explants by gibberellic acid. J. Plant Growth Regul. 4: 19–27

    Article  Google Scholar 

  • Gibson M (1988) Dihaploid plant product from ovule culture sugar beet. Proc. N.D. Acad. Sci. Diol. Sei. 36: 171–175

    Google Scholar 

  • Guha S & Johri BM (1966) In vitro development of ovary and ovule of Allium cepa L. Phytomorfology. 16: 353–364

    Google Scholar 

  • Guha S & Maheshwari SC (1964) In vitro production of embryos from anthers of Datura. Nature. 204: 497

    Article  Google Scholar 

  • Guha S Maheshwari SC (1966) Cell division and differentiation of embryos in the pollen grains of Datura in vitro. Ibid. 212: 97–98

    Google Scholar 

  • Guo F, Jia XJ & Chen LX(1982) Induction of plantlets from isolated ovules of Hevea brasiliensis. Hereditas. 4: 27–28

    Google Scholar 

  • Guo JF, Shao MW, Ma LB & Zhang YQ (1994) A study on plant regeneration by culturing protoplast of sugarbeet. China Sugarbeet. 4: 6–9

    Google Scholar 

  • Heller R (1953) Recherches sur la nutrition minerale des tissus vegetaux cultives in vitro. Bot. Biol. et Veg. 14: 1–223

    Google Scholar 

  • Hill RA, Tuskan GA & Boe AA (1989) In vitro propagation of Hosta sieboldiana using excised ovaries from immature florets. Plant Cell Tiss.Org. Cult. 17: 71–75

    Google Scholar 

  • Holm PB, Knudsen S, Mouritzen P, Negri D, Olsen FL & Roue C (1994) Regeneration of fertile barley plants from mechanically isolated protoplasts of the fertilized egg cell. Plant Cell. 6: 531–543

    Article  PubMed  CAS  Google Scholar 

  • Honkanen J, Aapola A, Seppanen P, Tormala T, Wit JC, Esendam HF, Stravers LJM & De Wit JC (1992) Production of doubled haploid Gerbera clones. Acta Horticultural. 300: 341–346

    Google Scholar 

  • Hoseman D & Bossoutrot D (1983) Induction of haploid plants from in vitro culture of unpollinated beet (Beta vulgaris L.). Z.Pflanzenzucht. 91: 74–77

    Google Scholar 

  • Huang QT, Yang HY & Zhou C (1982) Embryological observations on ovary culture of unpollinated young flowers in Hordeum vulgare L. Acta Botanica Sinica. 24: 295–300

    Google Scholar 

  • Hvilkovskaya B (1982) Callus formation and plant regeneration from explants of mono (2n=x=12) and dihaploid (2n=x=24) plants of Solanum tuberosum. Cytologiya i Genetica. 16: 49–55

    Google Scholar 

  • Hvilkovskaya B & Zenkteler M (1983) Influence of some phytohormons to tissue cultured in vitro ovules of Solanum tuberosum (2n=x=24). Cytologiya i Genetica. 17: 12–19

    Google Scholar 

  • Kalinin FS, Davojan NI & Mosolygina MK (1991) Isolation vitality embryo sacs of plant. Materialy Vsesoyuznoi Konferencii po Selsko Hozyaistvennoi Biotehnologii (pp 69–70). Tselinograd.

  • Kojima A & Kawaguchi T (1988) Dihaploids derived from unpollinated ovule culture of Chines chives. Proc. 4th Allium Symp. (pp 212–217). Wellesbourne

  • Kuo CS (1982) The preliminary studies on culture of unfertilized ovary of rice in vitro. Acta Botanica Sinica. 24: 33–38

    Google Scholar 

  • Laurain D, Chenieux JC & Tremouillaux-Guiller J (1993) Direct embryogenesis from female haploid protoplasts of Ginkgo biloba L., a medicinal woody species. Plant Cell Repts. 12: 656–660

    Google Scholar 

  • Liang XQ, Zheng GR & Luo BX (1990) A preliminary study on in vitro culture of groundnut (Arachis hypogaea L.) ovules. Oil Crops of China. 4: 76–79

    Google Scholar 

  • Maheshwari P & Rangaswamy NS (1965) Embryology in relation to physiology and genetics. In: Preston RD (ed) Advances in Botanical Research, Vol 2 (pp 219–321). London

  • Meynet J & Sibi M (1984) Haploid plants from in vitro culture of unfertilized ovules in Gerbera gamesonii. Z. Pflanzenzuchtg. 93: 78–85

    Google Scholar 

  • Mezei S & Kovacev L (1994) In vitro gynogenesis of inbred sugarbeet lines. Selekcija i Semenarstvo. 1: 99–102

    Google Scholar 

  • Miller CO (1963) Kinetin and kinetin-like compounds. Mod. Meth. Pflanzenanal. 6: 194–202

    Google Scholar 

  • Mol R (1986) Isolation of protoplast from female gametophytes of Torenia fourneri. Plant Cell Repts. 5: 202–206

    Article  Google Scholar 

  • Mol R, Betka A & Wojciechowicz M (1995) Induction of autonomous endosperm in Lupinus Luteus, Helleborous niger and Melandrium album by in vitro culture of unpollinated ovaries. Sexual Plant Reproduction. 8: 273–277

    Google Scholar 

  • Morrison RA, Evans DA, Fan ZG, Biswas BB & Harris JR (1991) Haploid plants from tissue culture: application in crop improvement. In: Biswas BB (ed) Subcellular Biochemistry. Vol 17. Plant Genetic Engineering (pp 53–72). Plenum Press, USA

    Google Scholar 

  • Mukhambetzhanov SK (1992) Growth and morphogenesis of nonfertilized ovaries of wheat in vitro. PhD Thesis. Main Botanical Garden Acad. of Sci. of Kazakstan, Alma-Ata

  • Mukhambetzhanov SK, Erezhepov AE & Rakhimbaev I (1991) Nucellar and integumental embryony in vitro culture of unpollinated ovaries of wheat.Izvestiya Academii Nauk Kazahstana. 2: 84–86

    Google Scholar 

  • Mukhambetzhanov SK & Rakhimbaev I (1991) Cytoembryological observation in vitro development of embryo sac ofwheat. Izvetiya Academii Nauk Kazahstana. 6: 70–73

    Google Scholar 

  • Mullin NG& Srinivasan C (1979) Plantlets of “Caberner-Sauvuguo” grapes by nucellar embryony in vitro. Genet. et Amelior. Vigne 2-e Symp. Int. Bordeaux. (pp 11–15). Paris

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473

    Article  CAS  Google Scholar 

  • Nikitina NI, Fedorenko TS & Borovkov AYu (1989) Culture of nonfertilized ovules of sunflower. Nauchno-technicheski Bullyuten VNII Maslichnyh Cultur, 3: 14–16

    Google Scholar 

  • Nitsch C (1974) Pollen culture-a new technique formass production of haploid and homozygous plants. In: Guha KJ (ed) Haploid in Higer Plants: Advance and Potential (pp 123–135). Guelf. Univ. Press

  • Pavlova MK (1983) Culture in vitro of nonfertilized ovules of Crepis tectorum L. Materialy IV Vsesoyuznoi (USSR) Conf. (pp 101). Kishinev

  • Pavlova MK (1987) Culture of unfertilized gunaecia and seedbuds: Potential and prospects. Selskohozyaistvennaya Bilogiya. 1: 27–33

    Google Scholar 

  • Pavlova MK & Hvedynich OA(1984) Change of elements of embryo sac during cultivation nonfertilized ovaries of piper and tobacco. Materialy Vsesoyuznogo(USSR) Symp. (pp 45–46). Telavi

  • Picard E, Crambes E, Liu CS & Mihamou-Ziyyat A(1994) Evolution of haplodiploidisation methods and prospects for plant breeding. Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales. 188: 109–141

    Google Scholar 

  • Rahimbaev I & Tiwari S (1992) Anther and isolated microspore culture of barley. In: Polimbetova FA (ed) Biotechnology of Cereals (7–57). Gylym. Alma-Ata

  • Rangan TC (1982) Ovary, ovule and nucellus culture. In: Johri BM (ed) Exp. Embryol. Vascular Plants (pp 105–129)

  • Sachar RC & Kapoor M (1958) Influence of kinetin and gibberellic acid on the test-tube seeds of Cooperia pedunculata Herb. Naturwissenschaften. 45: 552–553

    Article  CAS  Google Scholar 

  • Sachar RC & Kapoor M (1959) In vitro culture of ovules of Zephyranthes. Phytomorfology. 9: 147–156

    CAS  Google Scholar 

  • San Noeum LH (1976) Haploides d' Hordeum vulgare L. par culture in vitro d' ovaries non fecondes. Ann.Amelior. Plant. 26: 751–754

    Google Scholar 

  • San Noeum LH (1979) In vitro induction of gynogenesis in higher plants. Broadening Genet. Base Crops: Proc. Conf. (pp 327–329). Wageningen

  • San Noeum L & Ahmadi (1980) Variability of doubled haploid from in vitro androgenesis and gynogenesis in Hordeum vulgare L. Colloque NSP, CNRS. Orsay

    Google Scholar 

  • Schum A, Mattiesch l, Timmann EM & Hofmann (1993) Regeneration of diploids via gynogenesis in Allium porrum L. Gartenbauwissenschaft. 58: 227–232

    Google Scholar 

  • Seigner E (1992) Conventional breeding and biotechnology join forces on the way to healthy plants. Monatsschrift fur Brauwissenchaft. 45: 242–251

    Google Scholar 

  • Shamina ZB (1981) Androgenesis and obtaining haploids from anther and microspore culture. In: Butenko RG (ed) Plant Cell Culture (pp 124–135). Nauka. Moscow

  • Sita Gl, Ravindran S, Prakash J & Pierik RLM (1991) Gynogenic plants from ovary culture of mulberry (Morus indica). In: Prakash J (ed) Horticulture-New Technologies and Applications. Proc. Int. Seminar on New Frontiers in Horticulture (pp 225–229). Bangalore, India. Kluwer Academic Publisher, Netherlands

    Google Scholar 

  • Sitbon M (1981) Production of haploid Gerbera jamesonii plants by in vitro culture unfertilized ovules. Agronomie. 1: 807–812

    Google Scholar 

  • Sunderland N & Dunwell JM (1978) Anther and pollen culture. In: Street HE (ed) Plant Tissue and Cell Culture (pp 223–264). Oxford

  • Svirshchevskaya AM, Kozyrevich TP & Bormotov VE (1993) Haploids in the culture of unfertilized ovules in beet. Doklady Akademii Nauk Belarusi. 37: 74–76

    Google Scholar 

  • Svirshchevskaya AM & Bormotov VE (1994) Features of regeneration following gynogenesis in sugarbeet. Doklady Akademii Nauk Belarusi. 38: 57–59

    Google Scholar 

  • Truong-Andre I & Demarly (1984) Obtaining plants by in vitro culture of unfertilized maize ovaries (Zea mays L.) and preliminary studies on the progeny of gynogenetic plant. Z. Pflanzenzucht. 92: 309–320

    Google Scholar 

  • Tulecke W (1964) A haploid tissue culture from the female gametophyte of Ginkgo biloba. Nature. 203: 94–95

    Article  Google Scholar 

  • Tyrnov VS & Alatorceva TA (1990) Corn ovaries culture in vitro as a model system for study of parthenogenesis. MaterialyII

  • Vsesoyuznogo (USSR) Soveshania. Genetica Razvitiya (pp 164–166). Tashkent

  • Uchimiya H, Kameya T & Takahashi N (1971) In vitro culture of unfertilized ovules in Solanum melongena and ovaries in Zea mays. Jap. J. Breed. 21: 247–250

    Google Scholar 

  • Wagner VT, Sohg, Matthys RE & Dumans C (1989) Observation on the isolated embryo sac of Zea mays L. Plant Sci. 59: 127–132

    Article  Google Scholar 

  • Wang CC & Kuang BG (1981) Induction of haploid plants from the female gametophyte of Hordeum vulgare L. Acta Botanica Sinica. 23: 329–330

    Google Scholar 

  • Wenzel G, Graner A, Fadel F, Zitzlsperger J, Foroughi-Wehr J & Moss JP (1992) Production and use of haploids in crop improvement. In: Biotechnology & Crop Improvement in Asia (pp 169–179). ICRISAT, India

  • Wernsman EA, Matzinger DF & Ruffu RC (1989) Androgenetic vs. gynogenetic doubled haploides of tobacco. Crop.Sci. 29: 1151–1155

    Article  Google Scholar 

  • White PhR (1943) Handbook of plant tissue culture. Jaques Cattell press, Vol 4 (pp 791–794)

    Google Scholar 

  • Wu BJ & Chen KC (1982) Cytological and embryological studies on haploid plant production from cultured unpollinated ovaries of Nicotiana tabacum L. Acta Botanica Sinica. 24: 125–129

    Google Scholar 

  • Yang HY & Zhou C (1982) In vitro induction of haploid plants from unpollinated ovaries and ovules. Theor. & Applied Genetica. 63: 97–104

    Article  Google Scholar 

  • Zenkteller M & Nitzsche W (1985) In vitro culture of ovules of Triticum aestivum at early stages of embryogenesis. Plant Cell Repts. 4: 168–171

    Article  Google Scholar 

  • Zhang SE, Zhang ZD, Liu ZY, Shen YZ & Si ZH (1993) Induction of haploid plants from unpollinated ovaries of male sterile lines of wheat in vitro. Hereditas-Beijing. 15: 20–23

    CAS  Google Scholar 

  • Zhou C & Yang HY (1980) In vitro induction of haploid plantlets from unpollinated young ovaries of Oryza sativa L. Acta Genetica Sinica. 7: 287–288

    Google Scholar 

  • Zhou C & Yang HY (1981) Induced of haploid plantlets by ovary culture. Plant Sci. Let. 20: 231–237

    Article  Google Scholar 

  • Zhou C& Yang HY (1981) Studies on the in vitro induction of callus from embryo sacs of rice. Hereditas. 3: 10–12

    Google Scholar 

  • Zhou JY, Ma H, Guo FX & Luo XT (1994) Effect of thidiazuron on somatic embryogenesis of Cayratia japonica. Plant Cell, Tiss. Org. Cult. 36: 73–79

    Article  CAS  Google Scholar 

  • Zhu ZC & Wu HS (1979) In vitro induction of haploid plantlets from unpollinated ovaries of Triticum aestivum and Nicotiana tabacum. Acta Genetica Sinica. 6: 181–184

    Google Scholar 

  • Zhu ZC, Liu ZY & Wu HS (1981) Development of embryoids from unpollinated Nicotiana ovaries cultured in vitro. Acta Botanica Sinica. 23: 499–501

    Google Scholar 

  • Zhu ZC, Wu HS, An QK & Liu ZY (1981) Induction of haploid plantlets from unpollinated ovaries of Triticum aestivum cultured in vitro. Acta Genetica Sinica. 8: 386–396

    Google Scholar 

  • Ziv M & Kahana O (1988) The role of peanut (Araschis hypogaea) ovular tissue in the photomorphogenetic responce of the embryo. Plant Sci. 25: 159–164

    Article  Google Scholar 

  • Znamenskaya VV, Podvigina OA & Zhuzhzhalova TP (1994) Production of new breeding material. Sakharnaya Svekla. 6: 8

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mukhambetzhanov, S.K. Culture of nonfertilized female gametophytesin vitro. Plant Cell, Tissue and Organ Culture 48, 111–119 (1997). https://doi.org/10.1023/A:1005838113788

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1005838113788

Navigation