Skip to main content
SpringerLink
Log in

Isolation of protoplasts from female gametophytes of Torenia fournieri

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Protoplasts from the cells of mature embryo sacs (ES-protoplasts) of Torenia fournieri were obtained during incubation of ovules in an enzyme solution. Four protoplasts which arose from each embryo sac were connected together after isolation, or aggregates of the egg cell protoplast and two synergide protoplasts dissociated from the protoplast of the central cell. The ES-protoplasts stayed viable for 2 weeks in culture, but they did not regenerate cell walls.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

ES:

embryo sac

FAA:

fixative (formalin : acetic acid : alcohol = 1 : 1 : 18)

FDA:

fluorescein diacetate

PAS:

periodic acid Schiff reaction

2,4-D:

2,4-dichlorophenoxyacetic acid

References

  • Guilford VB, Fisk EL (1951) Bull Torrey Bot Club 79: 6–24

    Google Scholar 

  • Hu SY, Li LG, Zhou C (1985) Acta Bot Sin 27: 337–344

    Google Scholar 

  • Potrykus I, Harms CT, and Lörz H (1979) Callus formation from cell culture protoplasts of corn (Zea mays L.) Theor Appl Genet 54: 209–214

    Google Scholar 

  • Tiwari SC (1982) Protoplasma 110: 1–4

    Google Scholar 

  • Tyrnov VS, Enaleeva NKh (1979) In: Theses of Reports, 3rd All-Union Conf on Plant Cell Culture (in Russ), Abovjan, pp 164–165

  • Tyrnov VS, Enaleeva NKh, Khokhlov SS (1975) In: Theses of Reports, XII Int Bot Congr (in Russ), Nauka, Leningrad, p 266

  • Van der Pluijm JE (1964) In: Linskens HF (ed) Pollen physiology and fertilization, North-Holland Publ Comp, Amsterdam, pp 8–16

    Google Scholar 

  • Yang HY, Zhou C (1982) Theor Appl Genet 63: 97–104

    Google Scholar 

  • Zhou C (1985) Acta Bot Sin 27: 258–262

    Google Scholar 

  • Zhou C, Yang HY (1984) Acta Biol Exp Sin 17: 141–147

    Google Scholar 

  • Zhou C, Yang HY (1985) Planta 165: 225–231

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Max-Planck-Institut für Züchtungsforschung, D-5000, Köln 30, Germany

    R. Mól

Authors
  1. R. Mól
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by I. Potrykus

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mól, R. Isolation of protoplasts from female gametophytes of Torenia fournieri . Plant Cell Reports 5, 202–206 (1986). https://doi.org/10.1007/BF00269119

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00269119

Keywords

Search

Navigation