Abstract
Haploid plants have been obtained from unpollinated ovules of onion cultured in vitro as here reported for the first time. Callus was not formed at any step of the process and the young developing embryos sprouted by splitting the ovules. From a total of about 26000 cultured ovules, 13 embryos were obtained and 7 of them developed into plants. Cytological observation of root apexes showed that 3 embryos out of 7 regenerated haploid plants (8 chromosomes). The yield of embryo induction was quite low (0.28% the best treatment) so further experiments are in progress to increase it.
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Abbreviations
- NAA:
-
naphthaleneacetic acid
- IAA:
-
indole-3yl-acetic acid
- 2iP:
-
N6-Δ2-isopentenyladenine
- TIBA:
-
2-3-5 trijodobenzoic acid
- adenine SO4 :
-
adenine sulphate · 2H2O
- ABA:
-
abscisic acid
- GA3 :
-
gibberellic acid
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Campion, B., Alloni, C. Induction of haploid plants in onion (Allium cepa L.) by in vitro culture of unpollinated ovules. Plant Cell Tiss Organ Cult 20, 1–6 (1990). https://doi.org/10.1007/BF00034750
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DOI: https://doi.org/10.1007/BF00034750