Abstract
Nonfouling thin films were prepared by the plasma deposition of tetraethylene glycol dimethyl ether (pp4G) on fluorinated ethylene propylene polymer (FEP) and glass substrates. Ordered cell patterns were created on these surfaces by microcontact printing of proteins. Pp4G was found to be stable in aqueous environments and resistant to an ethanol sterilization procedure, as verified by surface analysis. Pp4G also reduced nonspecific protein adsorption by more than 65-fold before and after sterilization. Despite the low adsorption of proteins to pp4G in solution, protein microcontact printing was achieved and we were able to print laminin, an adhesive extracellular matrix protein, from an elastomeric stamp onto pp4G. The printed laminin supported the attachment and spreading of cardiomyocytes and the nonprinted pp4G regions remained cell repulsive in culture conditions. Microscale patterns of cardiomyocytes were maintained on printed pp4G for more than 7 days. This cell patterning process should be viable for other cell types. The potential applications include tissue engineering and microdevices for biosensor, diagnostic, and pharmacological applications.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
S. B. Carter, Exp. Cell. Res. 48, 189 (1967).
S. B. Carter, Nature 213, 256 (1967).
S. B. Carter, Nature 225, 858 (1970).
A. Folch and M. Toner, Ann. Rev. Biomed. Eng. 2, 227 (2000).
L. E. Donald and A. H. Jeffrey, Ann. Rev. Mater. Sci. 26, 365 (1996).
S. Zhang, G. Wright, and Y. Yang, Biosens. Bioelectron. 15, 273 (2000).
Y. V. Pan, Y. Hanein, D. Leach-Scampavia, K. F. Bohringer, B. D. Ratner, and D. D. Denton, A precise technology for controlling protein adsorption and cell adhesion in bioMEMS. In IEEE MEMS Technical Digest, 435 (2001).
Y. V. Pan, Y. Hanein, D. Leach-Scampavia, K. F. Bohringer, D. D. Denton, and B. D. Ratner, Micro-scale cell patterning on a nonfouling surface with micro-device applications. Biomaterials (2002)(submitted).
Y. Hanein, Y. V. Pan, B. D. Ratner, D. D. Denton, and K. F. Bohringer, Micromaching of non-fouling coatings for bio-MEMS applications. Sensors and Actuators B 81, 49–54 (2001).
H. Lu, J. Homola, Y. V. Pan, B. D. Ratner, C. T. Campbell, and S. S. Yee, Surface functionalization for self-referencing surface plasmon resonance biosensors by RF-plasma-deposited thin films and self-assembled monolayers. Transactions of the Sixth World Biomaterials Congress, Society for Biomaterials, 151 (2000).
M. N. Mar, B. D. Ratner, and S. S. Yee, Sensors Actuators B, 54, 125 (1999).
C. D. James, R. C. Davis, L. Kam, H. G. Craighead, M. Isaacson, J. N. Turner, and W. Shain, Langmuir 14, 741 (1998).
A. Bernard, E. Delamarche, H. Schmid, B. Michel, H. R. Bosshard, and H. Biebuyck, Langmuir 14, 2225 (1998).
D. W. Branch, J. M. Corey, J. A. Weyhenmeyer, G. J. Brewer, and B. C. Wheeler, Med. Biol. Eng. Comput. 36, 135 (1998).
C. D. James, R. Davis, M. Meyer, A. Turner, S. Turner, G. Withers, L. Kam, G. Banker, H. Craighead, M. Issacson, J. Turner, and W. Shain, IEEE Trans. Biomed. Eng. 47, 17 (2000).
A. S. Blawas and W. M. Reichert. Biomaterials 19, 595 (1998).
G. P. Lopez, B. D. Ratner, C. D. Tidwell, C. L. Haycox, R. J. Rapoza and T. A. Horbett, J. Biomed. Mater. Res. 26, 415 (1992).
J. M. Harris, ed., Poly(ethylene glycol) Chemistry, Plenum, New York (1992).
F. E. Baily, Jr., and R. W. Callard, J. Appl. Polym. Sci. 1, 56 (1959).
N. B. Graham, N. E. Nwachuku, and D. J. Walsh, Polymer 23, 1345 (1982).
J. D. Andrade, V. Hlady, and S.-I. Jeon, Hydrophilic Polymers, Performance with Environmental Acceptance, J. E. Glass, ed., American Chemical Society Advances in Chemistry Series 248, (1996).
E. Johnston, Surface and biological properties of biofouling-resistant, poly(ethylene oxide)-like Plasma Deposited Films, Ph.D. thesis, University of Washington, Seattle, Washington (1997).
K. L. Prime and G. M. Whitesides, Science 252, 1164 (1991).
K. L. Prime and G. M. Whitesides, J. Am. Chem. Soc. 115, 10714 (1993).
E. E. Johnston, B. D. Ratner, and J. D. Breyer, Plasma Processing of Polymers, R. d'Agostino, P. Favia, and F. Fracassi, ed., NATO Science Series E: Applied Sciences 346 (1997).
D. Beyer, W. Knoll, H. Ringsdorf, J.-H. Wang, R. B. Timmons, and P. Sluka, J. Biomed. Mater. Res. 36, 181 (1997).
Y. J. Wu, R. B. Timmons, J. S. Jen, and F. Z. Molock, Coll. Surf. B 18, 235 (2000).
K. R. Kamath, M. J. Danilich, R. E. Marchant, and K. Park, J. Biomater. Sci. Polymer Edn. 7, 977 (1996).
T. A. Horbett, Techniques of Biocompatibility Testing, D. F. Williams, ed., CRC Press, Boca Raton, Florida (1986).
T. C. McDevitt, J. C. Angello, M. L. Whitney, H. Reinecke, S. D. Hauschka, C. E. Murry, and P. S. Stayton, In vitro generation of differentiated cardiac myofibers on micropatterned laminin surfaces, J. Biomed. Mater. Res. 60, 472 (2002).
R. S. Kane, S. Takayama, E. Ostuni, D. E. Ingber, and G. M. Whitesides, Biomaterials 20, 2363 (1999).
K. Iwaki, V. P. Sukhatme, H. E. Shubeita, and K. R. Chien, J. Biol. Chem. 265, 13809 (1990).
H. Reinecke, M. Zhang, T. Bartosek, and C. E. Murry, Circulation 100, 193 (1999).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Pan, Y.V., McDevitt, T.C., Kim, T.K. et al. Micro-Scale Cell Patterning on Nonfouling Plasma Polymerized Tetraglyme Coatings by Protein Microcontact Printing. Plasmas and Polymers 7, 171–183 (2002). https://doi.org/10.1023/A:1016295419941
Issue Date:
DOI: https://doi.org/10.1023/A:1016295419941