Abstract
Pichia anomala, isolated from dried flower buds of Woodfordia fruticosa, produced a high activity of an intracellular phytase, at 68 U per g dry biomass, when grown at 20 °C for 24 h in a medium containing glucose (40 g l−1) and beef extract (10 g l−1) supplemented with Fe2+ (0.15 mM). Partially purified phytase was optimally active at 60 °C and pH 4 with a half life of 7 days at 60 °C. It retained 85% of its activity at 80 °C for 15 min. The enzyme is suitable for supplementing animal feeds to improve the availability of phosphate from phytate.
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Vohra, A., Satyanarayana, T. Phytase production by the yeast, Pichia anomala . Biotechnology Letters 23, 551–554 (2001). https://doi.org/10.1023/A:1010314114053
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DOI: https://doi.org/10.1023/A:1010314114053