Abstract
Transformation of DNA into microbial cells via heat-shock approach has been well established in the field of molecular biology for decades. Herein we described an unexpected finding that heat-shock may not play an essential role in the transformation process. This observation was verified via UV-Vis and fluorescence spectroscopies, and confocal microscopy images for various DNAs and bacterial cells. The non-heat-shock approach proposed in this study can be a convenient and beneficial modification for DNA transformation, especially for those laboratories lacking ice machine and heat-shock equipment.
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Supported by the National Natural Science Foundation of China(No.21372097) and the Grant from the Center of International Mobility(CIMO) of Finland.
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Wang, S., Li, Z. & Chen, Z. Chemotransformation of bacterial cells without heat-shock. Chem. Res. Chin. Univ. 33, 160–165 (2017). https://doi.org/10.1007/s40242-017-6403-8
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DOI: https://doi.org/10.1007/s40242-017-6403-8