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Adhesion of Lactobacillus reuteri strain Lr1 to equine epithelial cells and competitive exclusion of Clostridium difficile from the gastro-intestinal tract of horses

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Abstract

Lactobacillus reuteri Lr1, isolated from healthy horses, remained viable after 2 h at pH 2.0 and in the presence of 1.5 % (w/v) bile. Strain Lr1 survived passage through the equine gastro-intestinal tract (GIT). However, no viable cells of L. reuteri Lr1 were detected on the third day after administration, suggesting that the strain did not colonise the GIT for longer than two days. Strain Lr1 adhered to non-viable, but not to viable, buccal epithelial cells in vitro. Adherence of strain Lr1 to buccal epithelial cells increased 25 % after treatment of the bacterial cells with pepsin. Treatment with pronase prevented the adhesion to epithelial cells. This suggested that specific proteins on the cell surface of L. reuteri Lr1 are involved in adhesion to epithelial cells. Strain Lr1 aggregated with Clostridium difficile C6, isolated from the GIT of a horse that died from severe colic. Adherence of C. difficile C6 to epithelial cells declined from 60 % to 3 % when challenged with L. reuteri Lr1 and the number of viable clostridia decreased tenfold during dosage. Red blood cell, haemoglobin and haemocrit levels were significantly (P ≤ 0.05) lower after dosage with L. reuteri Lr1. Cholesterol and glucose levels were mildly elevated for one day during dosage, but decreased significantly thereafter to levels similar than before dosage. Genes encoding adhesion to collagen, production of aggregation substances, cytolysin and β hemolysin III, resistance to vancomycin A, B and C, and gelatinase activity were not detected, suggesting that L. reuteri Lr1 is a potential probiotic that may be used to control C. difficile cell numbers in the GIT.

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Acknowledgements

The assistance of Vino Pretorius, part time technical assistant, is much appreciated.

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Authors and Affiliations

  1. Department of Microbiology, University of Stellenbosch, Stellenbosch, 7600, South Africa

    Leon M. T. Dicks & Marlie Botha

  2. Department of Physiological Sciences, University of Stellenbosch, Stellenbosch, 7600, South Africa

    Ben Loos & Carine Smith

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  1. Leon M. T. Dicks
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  2. Marlie Botha
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  3. Ben Loos
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  4. Carine Smith
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Correspondence to Leon M. T. Dicks.

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Dicks, L.M.T., Botha, M., Loos, B. et al. Adhesion of Lactobacillus reuteri strain Lr1 to equine epithelial cells and competitive exclusion of Clostridium difficile from the gastro-intestinal tract of horses. Ann Microbiol 65, 1087–1096 (2015). https://doi.org/10.1007/s13213-014-0954-4

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  • DOI: https://doi.org/10.1007/s13213-014-0954-4

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