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Simultaneous detection of Staphylococcus aureus enterotoxin C-producing strains from clinical and environmental samples by multiplex PCR assay

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Abstract

A multiplex PCR (MPCR) assay was developed for the detection of Staphylococcus aureus using selected primers designated on the genus-specific gap gene, species-specific nuc gene and enterotoxin-producing EntC1 gene. The internal regions amplified had a product size of 933 bp, 273 bp and 531 bp, respectively. This MPCR assay had a sensitivity to detect 10 cells of S. aureus and 100 pg of genomic DNA of S. aureus. The MPCR assay was found to be specific for S. aureus, as it yielded negative results with other tested bacterial pathogens such as Salmonella typhimurium, Vibrio cholerae and Escherichia coli. Therefore, this developed MPCR assay could be used for the simultaneous detection of S. aureus enterotoxin C-producing strains from clinical and environmental samples.

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Acknowledgement

The authors wish to thank the Dean, Fisheries College and Research Institute, Tuticorin, India for providing necessary support to carry out this study.

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Authors and Affiliations

  1. Department of Fish Processing Technology; Fisheries College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University, Tuticorin, 628 008, India

    Geevaretnam Jeyasekaran, Kannan Thirumalai Raj, Robinsondhas Jeya Shakila, Albin Jemila Thangarani & Selvaramesh Karthika

  2. Department of Microbiology, Thoothukudi Government Medical College, Tuticorin, 628 008, India

    Michelraj Luzi

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  1. Geevaretnam Jeyasekaran
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  2. Kannan Thirumalai Raj
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  3. Robinsondhas Jeya Shakila
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  4. Albin Jemila Thangarani
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  5. Selvaramesh Karthika
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  6. Michelraj Luzi
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Correspondence to Geevaretnam Jeyasekaran.

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Jeyasekaran, G., Raj, K.T., Shakila, R.J. et al. Simultaneous detection of Staphylococcus aureus enterotoxin C-producing strains from clinical and environmental samples by multiplex PCR assay. Ann Microbiol 61, 585–590 (2011). https://doi.org/10.1007/s13213-010-0176-3

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  • DOI: https://doi.org/10.1007/s13213-010-0176-3

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