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Effect of Angiogenesis Induced by Consecutive Intramuscular Injections of Vascular Endothelial Growth Factor in a Hindlimb Ischemic Mouse Model

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Abstract

Purpose

Angiogenesis plays a major role in various physiological and pathological situations. Thus, an angiogenic therapy with vascular endothelial growth factor (VEGF) has been commonly recommended as a representative therapeutic solution to recover the insufficient blood supply of collateral vessels in an ischemic lesion. In this study, the injection method and injection time point of VEGF proteins were focused to discover how to enhance the angiogenic effect with VEGF.

Methods

Mouse models (n = 15) were divided into control, VEGF treatment by intra-venous injection (VEGF-IV) and VEGF treatment by intra-muscular injection (VEGF-IM). Right proximal femoral arteries of mice were firmly sutured to obstruct arterial blood-flow. In the VEGF-IV treatment group, VEGF proteins were injected into the tail vein and, in the VEGF-IM treatment group, VEGF proteins were directly injected into the ischemic site of the right thigh after postoperative day 5, 10, 15, 20 and 25 follow-ups. Blood-flow images were acquired by 99mTc Gamma Image Acquisition System to compare the ischemic-to-non-ischemic bloodstream ratio at postoperative days 5, 15, and 30.

Results

VEGF-IM treatment significantly induced higher an angiogenic effect rather than both the control group (P = 0.008) and VEGF-IV treatment group (P = 0.039) at the 30th day.

Conclusion

During all experiments, angiogenesis of VEGF-IM treatment represented the most evident effect compared with control and VEGF-IV group in a mouse model of hindlimb ischemia.

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Acknowledgments

This study was supported by the Radiation Technology R&D program through the National Research Foundation of Korea Grant funded by the Ministry of Education, Science and Technology (2012-M2A2A7035779, 2011-0028581, 2009-0078422) and Research Funds of Chonbuk National University in 2012.

Conflicts of Interest

Tai Kyoung Lee, Chang-Moon Lee, Hyosook Hwang, Kyung Sook Na, JeongIl Kwon, Hwan-Seok Jeong, Philsun Oh, Hee Kwon Kim, Seok Tae Lim, Myung-Hee Sohn, and Hwan-Jeong Jeong declare that they have no conflict of interest.

Ethical Statement

This study was approved by the animal ethics committee in our hospital and was performed in accordance with the ethical standards laid down in the 1964 Declaration of Helsinki and its later amendments.

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Authors and Affiliations

  1. Department of Nuclear Medicine, Research Institute of Clinical Medicine, Cyclotron Research Center, Institute for Medical Sciences, Molecular Imaging & Therapeutic Medicine Research Center, Chonbuk National University Medical School and Hospital, Jeonju, South Korea

    Tai Kyoung Lee, Hyosook Hwang, Kyung Sook Na, JeongIl Kwon, Hwan-Seok Jeong, Philsun Oh, Hee Kwon Kim, Seok Tae Lim, Myung-Hee Sohn & Hwan-Jeong Jeong

  2. Department of Biomedical Engineering, Chonnam National University, Yeosu, South Korea

    Chang-Moon Lee

  3. Department of Nuclear Medicine, Chonbuk National University Medical School and Hospital, 634-18, Geumam-dong, Dukjin-gu, Jeonju, Republic of Korea

    Hwan-Jeong Jeong

Authors
  1. Tai Kyoung Lee
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  2. Hyosook Hwang
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  3. Kyung Sook Na
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  4. JeongIl Kwon
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  6. Philsun Oh
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  7. Hee Kwon Kim
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  8. Seok Tae Lim
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  9. Myung-Hee Sohn
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  11. Chang-Moon Lee
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Corresponding author

Correspondence to Hwan-Jeong Jeong.

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Lee, T.K., Hwang, H., Na, K.S. et al. Effect of Angiogenesis Induced by Consecutive Intramuscular Injections of Vascular Endothelial Growth Factor in a Hindlimb Ischemic Mouse Model. Nucl Med Mol Imaging 48, 225–229 (2014). https://doi.org/10.1007/s13139-014-0273-5

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  • DOI: https://doi.org/10.1007/s13139-014-0273-5

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