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Cellulolytic activity in the hepatopancreas of Chionoecetes opilio and Chionoecetes japonicus: enzymatic adaptations to deep sea environment

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  • Chemistry and Biochemistry
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Abstract

Chionoecetes opilio and Chionoecetes japonicus are closely related crab species that inhabit bathymetrically distinct habitats. C. opilio is caught mainly in waters shallower than 500 m, whereas C. japonicus largely resides at depths below 500 m. In this study, the activity of partially purified cellulase (β-1,4-glucanase) from the hepatopancreas of C. opilio (CoCel) and C. japonicus (CjCel) was investigated under high static pressure. SDS-PAGE-zymogram analysis revealed a major band in both species, with a calculated molecular mass of 41 kDa. In tests under various static pressures from 0.1 to 200 MPa, CoCel maintained 80 % of its activity up to 20 MPa, but activity decreased sharply to 20 % at 200 MPa in a pressure-dependent manner. In contrast, CjCel was less sensitive to high pressure, and maintained 65 % activity at 200 MPa. The activity of both CoCel and CjCel followed Michaelis–Menten kinetics at normal pressure levels; however, enzymatic activity of both CoCel and CjCel was suppressed in a non-competitive manner. These results suggest that CjCel, which can maintain normal activity under extremely high static pressure, have become highly adapted to the deep sea environment compared with CoCel.

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Acknowledgments

The authors would like to thank Professor Fumiyoshi Abe (Department of Chemistry and Biological Science, College of Science and Engineering, Aoyama Gakuin University) for his useful advice. The authors are grateful to Hideaki Yamada from the Tottori Fisheries Experimental Station for kindly providing the experimental samples for preliminary study. This work was supported in part by the Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research (JSPS KAKENHI; Grant Number 26520308).

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Authors and Affiliations

  1. Laboratory of Aquatic Product Utilization, Graduate School of Agriculture, Kochi University, Monobeotsu 200, Nankoku, Kochi, 783-8502, Japan

    Kohsuke Adachi, Kento Tanimura, Kou Ikejima & Katsuji Morioka

  2. Aoyama-Gakuin University, Sagamihara Campus L617, 5-10-1 Fuchinobe, Chuo, Sagamihara, Kanagawa, 252-5258, Japan

    Toshiyuki Mitsui

  3. National Research Institute of Fisheries Science, Fukuura 2-12-4, Kanazawa-ku, Yokohama, Kanagawa, 236-8648, Japan

    Takami Morita

  4. Japan Sea National Fisheries Research Institute, Fisheries Research Agency Japan, 1-5939-22 Suido-cho, Chuo-ku, Niigata, Niigata, 951-8121, Japan

    Ikuko Yosho

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  1. Kohsuke Adachi
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  2. Kento Tanimura
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  3. Toshiyuki Mitsui
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  4. Takami Morita
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  5. Ikuko Yosho
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  6. Kou Ikejima
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  7. Katsuji Morioka
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Correspondence to Kohsuke Adachi.

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Adachi, K., Tanimura, K., Mitsui, T. et al. Cellulolytic activity in the hepatopancreas of Chionoecetes opilio and Chionoecetes japonicus: enzymatic adaptations to deep sea environment. Fish Sci 82, 835–841 (2016). https://doi.org/10.1007/s12562-016-1014-8

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  • DOI: https://doi.org/10.1007/s12562-016-1014-8

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