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Transformed Root Extract of Leonurus sibiricus Induces Apoptosis through Intrinsic and Extrinsic Pathways in Various Grades of Human Glioma Cells

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Pathology & Oncology Research

Abstract

This study determines the influence of transformed root (TR) extract of Leonurus sibiricus L. on various grades (I-III) of human glioma cells derived from patients. This plant occurs in southern Asia and Siberia and is widely used as a medicinal plant with various biological activities. Chromatographic profile of TR extract have revealed the presence of various polyphenolic compounds (4-hydroxybenzoic acid, gentisic acid, vanilic acid, 1,3-dicaffeoylquinic acid, α-resorcylic acid). We found TR root extract to have antiproliferative activity on glioma cells after 24 h of treatment. TR root extract induces apoptosis on various grades (I-III) of human glioma cells by the generation of reactive oxygen species (ROS) along with concurrent loss of mitochondrial membrane potential, enhanced S and G2/M phases of the cell cycle, and altered mRNA levels of Bax, Bcl-2, p53, Cas-3, Cas-8 and Cas-9 factors involved in apoptosis. This work for the first time demonstrate that TR extract from L. sibiricus root has the potential to activate apoptosis in grade I-III human glioma cells through the intrinsic and extrinsic pathways.

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Authors and Affiliations

  1. Department of Biology and Pharmaceutical Botany, Medical University of Lodz, Muszyńskiego Street 1, 90-151, Łódź, Poland

    Przemysław Sitarek, Ewa Skała & Halina Wysokińska

  2. Department of Molecular Genetics, University of Lodz, Łódź, Poland

    Monika Toma & Tomasz Śliwiński

  3. Department of Plant Physiology and Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Łódź, Poland

    Marzena Wielanek

  4. Department of Medical Biochemistry, Medical University of Lodz, Łódź, Poland

    Janusz Szemraj

  5. Department of Microbiology and Immunology, and Fels Institute for Cancer Research, School of Medicine, Temple University, Philadelphia, PA, USA

    Tomasz Skorski

  6. Department of Pneumology and Allergy, 1st Chair of Internal Medicine, Medical University of Lodz, Łódź, Poland

    Adam J. Białas

  7. Department of Genetics and Plant Molecular Biology and Biotechnology, The University of Lodz, Łódź, Poland

    Tomasz Sakowicz & Tomasz Kowalczyk

  8. Department of Neurosurgery, Surgery of Spine and Peripheral Nerves, Medical University of Łódź, University Hospital WAM-CSW, Łódź, Poland

    Maciej Radek

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  1. Przemysław Sitarek
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  2. Ewa Skała
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Correspondence to Przemysław Sitarek.

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Sitarek, P., Skała, E., Toma, M. et al. Transformed Root Extract of Leonurus sibiricus Induces Apoptosis through Intrinsic and Extrinsic Pathways in Various Grades of Human Glioma Cells. Pathol. Oncol. Res. 23, 679–687 (2017). https://doi.org/10.1007/s12253-016-0170-6

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  • DOI: https://doi.org/10.1007/s12253-016-0170-6

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